Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 4,459,959 G→T P111P (CCC→CCA)  fbp ← fructose‑1,6‑bisphosphatase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,459,9590GT100.0% 108.2 / NA 62P111P (CCC→CCA) fbpfructose‑1,6‑bisphosphatase I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (0/62);  total (0/62)

ACGAGCCATCCAGGGGGTCCATCAGCACCACGTATTTTG  >  W3110S.gb/4459946‑4459984
             |                         
aCGAGCCATCCAGTGTGTCCATCAGCACCACGTATTTTg  <  1:34548/39‑1 (MQ=37)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTAttttt  <  1:20521/39‑2 (MQ=38)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:6244/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:10688/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:37023/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:37762/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:39105/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:3993/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:49775/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:50677/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:51500/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:51531/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:53714/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:57834/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:60456/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:6057/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:62049/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:36263/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:66949/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:67518/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:69168/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:71339/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:72162/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:7598/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:78309/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:79426/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:85248/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:87412/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:87813/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:88412/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:9227/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:23420/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:12202/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:1266/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:14458/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:14628/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:15520/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:16468/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:17658/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:18227/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:19642/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:19997/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:21365/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:22191/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:23011/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:3538/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:24203/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:25272/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:25488/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:28354/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:28568/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:30922/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:31813/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:32834/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:33746/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:34123/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:34510/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:34979/39‑1 (MQ=255)
aCGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:35353/39‑1 (MQ=255)
 cGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:72037/38‑1 (MQ=255)
 cGAGCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:8362/38‑1 (MQ=255)
    gCCATCCAGTGGGTCCATCAGCACCACGTATTTTg  <  1:55260/35‑1 (MQ=255)
             |                         
ACGAGCCATCCAGGGGGTCCATCAGCACCACGTATTTTG  >  W3110S.gb/4459946‑4459984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑