Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,462,994	C→T	A118A (GCC→GCT)	pmbA →	predicted peptidase required for the maturation and secretion of the antibiotic peptide MccB17

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,462,994	0	C	T	100.0%	101.9 / NA	58	A118A (GCC→GCT)	pmbA	predicted peptidase required for the maturation and secretion of the antibiotic peptide MccB17
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (58/0);  total (58/0)

CCAGATCCCTGTGCCGGCGTGGCAGACAAAGAGCTGCTGGCCTTTGACGCACCAGATCTCGA  >  W3110S.gb/4462953‑4463014                                          |                     ccAGATGCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:6764/1‑62 (MQ=16) ccAGATCCGTATGCAGGCGTTGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:65501/1‑62 (MQ=16) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGAc                >  1:59476/1‑48 (MQ=25) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGAtcttgg  >  1:43368/1‑59 (MQ=19) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:5727/1‑62 (MQ=19) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:56390/1‑62 (MQ=19) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:57346/1‑62 (MQ=19) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:58604/1‑62 (MQ=19) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:59646/1‑62 (MQ=19) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:60139/1‑62 (MQ=19) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:60247/1‑62 (MQ=19) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:60312/1‑62 (MQ=19) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:60931/1‑62 (MQ=19) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:61378/1‑62 (MQ=19) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:61586/1‑62 (MQ=19) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:61793/1‑62 (MQ=19) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:64537/1‑62 (MQ=19) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:66107/1‑62 (MQ=19) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:68292/1‑62 (MQ=19) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:71232/1‑62 (MQ=19) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:71587/1‑62 (MQ=19) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:74554/1‑62 (MQ=19) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:76912/1‑62 (MQ=19) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:78157/1‑62 (MQ=19) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:79072/1‑62 (MQ=19) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:81406/1‑62 (MQ=19) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:82231/1‑62 (MQ=19) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:82572/1‑62 (MQ=19) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:85420/1‑62 (MQ=19) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:88516/1‑62 (MQ=19) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:30333/1‑62 (MQ=19) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:12746/1‑62 (MQ=19) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:15492/1‑62 (MQ=19) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:15618/1‑62 (MQ=19) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:18589/1‑62 (MQ=19) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:19214/1‑62 (MQ=19) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:20109/1‑62 (MQ=19) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:21812/1‑62 (MQ=19) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:2300/1‑62 (MQ=19) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:24063/1‑62 (MQ=19) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:2411/1‑62 (MQ=19) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:25932/1‑62 (MQ=19) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:29879/1‑62 (MQ=19) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:29886/1‑62 (MQ=19) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:32250/1‑62 (MQ=19) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:32795/1‑62 (MQ=19) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:34221/1‑62 (MQ=19) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:36193/1‑62 (MQ=19) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:37974/1‑62 (MQ=19) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:38881/1‑62 (MQ=19) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:38970/1‑62 (MQ=19) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:42846/1‑62 (MQ=19) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:49815/1‑62 (MQ=19) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:52356/1‑62 (MQ=19) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:53600/1‑62 (MQ=19) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:54218/1‑62 (MQ=19) ccAGATCCGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGATCTTGa  >  1:54220/1‑62 (MQ=19) ccAGATCAGTATGCAGGCGTGGCAGACAAAGAACTGCTGGCTTTTGACGCGCCGGAtcttg   >  1:11883/1‑59 (MQ=16)                                          |                     CCAGATCCCTGTGCCGGCGTGGCAGACAAAGAGCTGCTGGCCTTTGACGCACCAGATCTCGA  >  W3110S.gb/4462953‑4463014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑