Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,485,919	C→T	T867T (ACG→ACA)	valS ←	valyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,485,919	0	C	T	100.0%	131.4 / NA	73	T867T (ACG→ACA)	valS	valyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/73);  total (0/73)

TTGTTGATGAGGCCAGCCATCGGGATCAGCAGCTCTGCACCGTCGATGATCTTCGTAA  >  W3110S.gb/4485866‑4485923                                                      |     ttgttgATGATGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:85792/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:55793/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:63768/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:63720/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:62078/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:60546/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:5751/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:57029/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:5633/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:56263/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:65691/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:54163/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:51676/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:51017/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:45454/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:45309/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:10297/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:41678/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:43062/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:70778/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:70901/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:71293/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:71776/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:719/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:7700/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:78477/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:79625/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:80991/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:82937/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:83210/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:8364/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:87165/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:8942/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:9248/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:23385/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:41157/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:29989/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:28599/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:28480/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:27055/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:26271/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:25547/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:23415/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:30255/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:23067/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:21887/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:1908/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:17453/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:16498/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:15337/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:13591/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:11328/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:32561/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:40769/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:39866/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:36988/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:3625/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:35689/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:34769/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:33698/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:32940/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:31791/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:3174/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:31199/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:31174/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:31159/58‑1 (MQ=255) ttgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGGCGACGATCTTTGTaa  <  1:15837/58‑1 (MQ=255)  tgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:61810/57‑1 (MQ=255)  tgttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:30203/57‑1 (MQ=255)  tgttgATGAGGCCTGCCATAGGGATCAACAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:60933/57‑1 (MQ=255)   gttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:63221/56‑1 (MQ=255)    ttgATGAGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:58009/55‑1 (MQ=255)     tgatgaGGCCTGCCATAGGGATCAGCAGTTCTGCGCCGTCGACGATCTTTGTaa  <  1:79240/54‑1 (MQ=255)                                                      |     TTGTTGATGAGGCCAGCCATCGGGATCAGCAGCTCTGCACCGTCGATGATCTTCGTAA  >  W3110S.gb/4485866‑4485923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑