Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	474,209	C→A	S59S (TCG→TCT)	tesB ←	acyl‑CoA thioesterase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	474,209	0	C	A	100.0%	67.0 / NA	39	S59S (TCG→TCT)	tesB	acyl‑CoA thioesterase II
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (39/0);  total (39/0)

AATAATCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTAGCTGTGAAACGAATGTACCAGCC  >  W3110S.gb/474161‑474222                                                 |              aataatCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTAGCTGTGAAAAGAATGTACCAGcc  >  1:70274/1‑62 (MQ=255) aataatCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTAGCTGTGAAAAGAATGTACCAGcc  >  1:37421/1‑62 (MQ=255) aataatCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTAGCTGTGAAAAGAATGTACCAGcc  >  1:41146/1‑62 (MQ=255) aataatCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTAGCTGTGAAAAGAATGTACCAGcc  >  1:48702/1‑62 (MQ=255) aataatCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTAGCTGTGAAAAGAATGTACCAGcc  >  1:51142/1‑62 (MQ=255) aataatCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTAGCTGTGAAAAGAATGTACCAGcc  >  1:5811/1‑62 (MQ=255) aataatCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTAGCTGTGAAAAGAATGTACCAGcc  >  1:58428/1‑62 (MQ=255) aataatCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTAGCTGTGAAAAGAATGTACCAGcc  >  1:62577/1‑62 (MQ=255) aataatCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTAGCTGTGAAAAGAATGTACCAGcc  >  1:68948/1‑62 (MQ=255) aataatCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTAGCTGTGAAAAGAATGTACCAGcc  >  1:12066/1‑62 (MQ=255) aataatCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTAGCTGTGAAAAGAATGTACCAGcc  >  1:74884/1‑62 (MQ=255) aataatCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTAGCTGTGAAAAGAATGTACCAGcc  >  1:74937/1‑62 (MQ=255) aataatCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTAGCTGTGAAAAGAATGTACCAGcc  >  1:75407/1‑62 (MQ=255) aataatCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTAGCTGTGAAAAGAATGTACCAGcc  >  1:78613/1‑62 (MQ=255) aataatCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTAGCTGTGAAAAGAATGTACCAGcc  >  1:81034/1‑62 (MQ=255) aataatCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTAGCTGTGAAAAGAATGTACCAGcc  >  1:87065/1‑62 (MQ=255) aataatCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTAGCTGTGAAAAGAATGTACCAGcc  >  1:88487/1‑62 (MQ=255) aataatCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTAGCTGTGAAAAGAATGTACCAGcc  >  1:973/1‑62 (MQ=255) aataatCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTAGCTGTGAAAAGAATGTACCAGcc  >  1:3387/1‑62 (MQ=255) aataatCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTAGCTGTGAAAAGAATGTACCAGcc  >  1:11847/1‑62 (MQ=255) aataatCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTAGCTGTGAAAAGAATGTACCAGcc  >  1:12505/1‑62 (MQ=255) aataatCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTAGCTGTGAAAAGAATGTACCAGcc  >  1:14786/1‑62 (MQ=255) aataatCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTAGCTGTGAAAAGAATGTACCAGcc  >  1:14793/1‑62 (MQ=255) aataatCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTAGCTGTGAAAAGAATGTACCAGcc  >  1:19650/1‑62 (MQ=255) aataatCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTAGCTGTGAAAAGAATGTACCAGcc  >  1:19884/1‑62 (MQ=255) aataatCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTAGCTGTGAAAAGAATGTACCAGcc  >  1:21507/1‑62 (MQ=255) aataatCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTAGCTGTGAAAAGAATGTACCAGcc  >  1:2317/1‑62 (MQ=255) aataatCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTAGCTGTGAAAAGAATGTACCAGcc  >  1:2590/1‑62 (MQ=255) aataatCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTAGCTGTGAAAAGAATGTACCAGcc  >  1:27945/1‑62 (MQ=255) aataatCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTAGCTGTGAAAAGAATGTACCAGcc  >  1:28028/1‑62 (MQ=255) aataatCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTAGCTGTGAAAAGAATGTACCAGcc  >  1:29408/1‑62 (MQ=255) aataatCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTAGCTGTGAAAAGAATGTACCAGcc  >  1:32188/1‑62 (MQ=255) aataatCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTAGCTGTGAAAAGAATGTACCAGcc  >  1:32191/1‑62 (MQ=255) aataatCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTAGCTGTGAAAAGAATGTACCAGcc  >  1:33094/1‑62 (MQ=255) aataatCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTAGCTGTGAAAAGAATGTACCAGcc  >  1:3496/1‑62 (MQ=255) aataatCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTAGCTGTGAAAAGAATGTACCAGc   >  1:77406/1‑61 (MQ=255) aataatCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTAGCTGTGAAAAGAATGTACCAGc   >  1:46541/1‑61 (MQ=255) aataatCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTAGCTGTGAAAAGAATGTACCAGc   >  1:45800/1‑61 (MQ=255) aataatCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTAGCTGTGAAAAGAATGTACCAGc   >  1:9979/1‑61 (MQ=255)                                                 |              AATAATCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTAGCTGTGAAACGAATGTACCAGCC  >  W3110S.gb/474161‑474222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑