Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 477,713 G→C T43T (ACC→ACG)  ylaB ← conserved inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb477,7130GC100.0% 194.9 / NA 103T43T (ACC→ACG) ylaBconserved inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (0/103);  total (0/103)

Only 100 of 103 total aligned reads displayed.

TCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAAC  >  W3110S.gb/477673‑477734
                                        |                     
tCGCCTCCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:69475/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:64456/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:71683/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:71578/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:7027/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:70247/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:70086/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:68045/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:67797/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:67044/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:6689/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:65854/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:72826/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:64203/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:63688/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:62560/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:62533/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:62377/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:6222/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:6030/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:59343/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:59130/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:56311/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:5433/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:79851/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:8709/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:86165/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:85910/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:85909/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:85788/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:84241/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:84206/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:82844/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:82601/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:82035/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:80144/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:10876/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:77408/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:76073/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:75932/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:7577/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:75683/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:75624/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:7474/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:7439/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:74260/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:73865/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:21063/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:31273/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:30686/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:28699/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:28397/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:26903/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:25260/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:24938/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:24320/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:23061/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:22156/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:21579/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:5337/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:20698/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:19729/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:19656/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:17783/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:16479/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:14110/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:14049/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:13383/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:1238/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:11560/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:10769/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:32408/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:5373/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:5225/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:51313/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:50109/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:49450/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:48922/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:48757/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:48653/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:46182/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:45433/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:4322/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:31912/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:34653/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:35050/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:35485/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:36961/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:38549/62‑2 (MQ=255)
tCGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:3987/62‑2 (MQ=255)
 cGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaatt  <  1:82180/61‑3 (MQ=255)
 cGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:61982/61‑2 (MQ=255)
 cGCCACCTTATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:70197/61‑2 (MQ=255)
         tATTGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:81877/53‑2 (MQ=255)
           ttGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:1780/51‑2 (MQ=255)
           ttGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:45245/51‑2 (MQ=255)
            tGGCCCGAATAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:80325/50‑2 (MQ=255)
                    aTAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:66045/42‑2 (MQ=38)
                     tAGCGACGCGGGAGGAATACGTATTCAGCTCTTCATTaaat  <  1:11287/41‑2 (MQ=37)
                         gACGCGGGAGGAATACGTATTCAGCTCTTCAATaaat  <  1:87684/37‑2 (MQ=37)
                                        |                     
TCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAAC  >  W3110S.gb/477673‑477734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑