Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 482,553 G→A L359L (CTG→TTG)  acrB ← multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb482,5530GA100.0% 169.7 / NA 93L359L (CTG→TTG) acrBmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (93/0);  total (93/0)

TGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAC  >  W3110S.gb/482549‑482610
    |                                                         
tGCAAGAACAGATACATTACCAGGAACACGAGgat                             >  1:23295/1‑35 (MQ=14)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCg            >  1:86259/1‑52 (MQ=18)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCat  >  1:74566/1‑61 (MQ=18)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCa   >  1:57601/1‑61 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCa   >  1:78516/1‑61 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCa   >  1:41571/1‑61 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCa   >  1:44838/1‑61 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCa   >  1:56920/1‑61 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCa   >  1:12004/1‑61 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:6912/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:72140/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:68334/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:67505/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:65383/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:71931/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:64718/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:7465/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:63972/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:59326/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:59060/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:5906/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:58572/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:57656/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:57529/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:53871/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:53287/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:50234/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:81428/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:9959/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:88624/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:88239/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:87535/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:87275/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:86592/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:86397/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:85075/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:84500/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:83910/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:73718/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:81349/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:80512/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:79777/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:78077/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:77680/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:77675/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:76530/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:76473/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:47046/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:739/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:19136/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:48073/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:28410/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:26251/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:24846/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:24019/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:23358/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:22561/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:19493/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:1949/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:19368/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:28958/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:18936/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:17634/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:17519/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:16332/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:16082/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:15805/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:15418/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:13416/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:13247/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:11273/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:38241/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:47787/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:10982/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:45758/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:45687/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:44671/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:43818/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:4339/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:42875/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:39506/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:29239/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:38170/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:37150/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:35791/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:34903/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:3486/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:34762/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:33088/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:32816/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:31348/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc  >  1:31102/1‑62 (MQ=255)
tGCAAGAACAGATACATTACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTAAACCAc  >  1:12328/1‑62 (MQ=18)
    |                                                         
TGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAC  >  W3110S.gb/482549‑482610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑