Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	519,362	C→T	L136L (CTG→TTG)	ybbA →	predicted transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	519,362	0	C	T	100.0%	64.3 / NA	39	L136L (CTG→TTG)	ybbA	predicted transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/39);  total (0/39)

GGAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATC  >  W3110S.gb/519319‑519380                                            |                   agAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGTTGGGTAAACGTCTTGatc  <  1:65120/61‑1 (MQ=255) agAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGTTGGGTAAACGTCTTGatc  <  1:53528/61‑1 (MQ=255) agAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGTTGGGTAAACGTCTTGatc  <  1:55409/61‑1 (MQ=255) agAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGTTGGGTAAACGTCTTGatc  <  1:56603/61‑1 (MQ=255) agAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGTTGGGTAAACGTCTTGatc  <  1:61163/61‑1 (MQ=255) agAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGTTGGGTAAACGTCTTGatc  <  1:61628/61‑1 (MQ=255) agAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGTTGGGTAAACGTCTTGatc  <  1:63421/61‑1 (MQ=255) agAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGTTGGGTAAACGTCTTGatc  <  1:63648/61‑1 (MQ=255) agAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGTTGGGTAAACGTCTTGatc  <  1:64603/61‑1 (MQ=255) agAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGTTGGGTAAACGTCTTGatc  <  1:54807/61‑1 (MQ=255) agAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGTTGGGTAAACGTCTTGatc  <  1:73613/61‑1 (MQ=255) agAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGTTGGGTAAACGTCTTGatc  <  1:77299/61‑1 (MQ=255) agAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGTTGGGTAAACGTCTTGatc  <  1:79348/61‑1 (MQ=255) agAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGTTGGGTAAACGTCTTGatc  <  1:81963/61‑1 (MQ=255) agAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGTTGGGTAAACGTCTTGatc  <  1:82452/61‑1 (MQ=255) agAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGTTGGGTAAACGTCTTGatc  <  1:84717/61‑1 (MQ=255) agAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGTTGGGTAAACGTCTTGatc  <  1:9157/61‑1 (MQ=255) agAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGTTGGGTAAACGTCTTGatc  <  1:35078/61‑1 (MQ=255) agAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGTTGGGTAAACGTCTTGatc  <  1:13117/61‑1 (MQ=255) agAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGTTGGGTAAACGTCTTGatc  <  1:1714/61‑1 (MQ=255) agAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGTTGGGTAAACGTCTTGatc  <  1:18923/61‑1 (MQ=255) agAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGTTGGGTAAACGTCTTGatc  <  1:29058/61‑1 (MQ=255) agAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGTTGGGTAAACGTCTTGatc  <  1:32947/61‑1 (MQ=255) agAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGTTGGGTAAACGTCTTGatc  <  1:34626/61‑1 (MQ=255) agAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGTTGGGTAAACGTCTTGatc  <  1:53223/61‑1 (MQ=255) agAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGTTGGGTAAACGTCTTGatc  <  1:35119/61‑1 (MQ=255) agAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGTTGGGTAAACGTCTTGatc  <  1:35151/61‑1 (MQ=255) agAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGTTGGGTAAACGTCTTGatc  <  1:37114/61‑1 (MQ=255) agAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGTTGGGTAAACGTCTTGatc  <  1:39268/61‑1 (MQ=255) agAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGTTGGGTAAACGTCTTGatc  <  1:4307/61‑1 (MQ=255) agAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGTTGGGTAAACGTCTTGatc  <  1:44073/61‑1 (MQ=255) agAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGTTGGGTAAACGTCTTGatc  <  1:49889/61‑1 (MQ=255) agAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGTTGGGTAAACGTCTCGatc  <  1:82110/61‑1 (MQ=255)  gAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGTTTGGTAAACGTCTTGatc  <  1:80411/61‑1 (MQ=255)  gAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGTTGGGTAAACGTCTTGatc  <  1:75704/61‑1 (MQ=255)  gAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGTTGGGTAAACGTCTTGatc  <  1:4920/61‑1 (MQ=255)  gAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGTTGGGTAAACGTCTTGatc  <  1:12448/61‑1 (MQ=255)  gAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGTGGGGTAAACGTCTTGatc  <  1:52913/61‑1 (MQ=255)             cGGGGCGAAAGCGTTGCTCGAACAGTTAGGGTTGGGTAAACGTCTTGatc  <  1:87166/50‑1 (MQ=255)                                            |                   GGAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATC  >  W3110S.gb/519319‑519380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑