Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,104,888	2 bp→TC	coding (201‑202/456 nt)	csgA →	cryptic curlin major subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,104,888	0	C	T	100.0%	94.4 / NA	54	D67D (GAC→GAT)	csgA	cryptic curlin major subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/54);  total (0/54)
*	W3110S.gb	1,104,889	0	T	C	100.0%	99.5 / NA	54	L68L (TTG→CTG)	csgA	cryptic curlin major subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/54);  total (0/54)

ACTTGCTCTGCAAACTGATGCCCGTAACTCTGACTTGACTATTACCCAGCATGGCGGCGG  >  W3110S.gb/1104855‑1104914                                  ||                          tcTTGCTCTGCAAGCTGACGCCCGTAACTCTGATCTGACCATTACCCAGCAcggcggcgg  <  1:32857/59‑1 (MQ=255)       tctGCAAGCTTACGCCCGTAACTCTGATCTGACCATTACCCAGCAcggcggcgg  <  1:87233/54‑1 (MQ=255)       tctGCAAGCTGACGCCCGTAACTCTTATCTGACCATTACCCAGCAcggcggcgg  <  1:18201/54‑1 (MQ=255)       tctGCAAGCTGACGCCCGTAACTCTGATCTGACCATTACCCAGCAcggcggcgg  <  1:50384/54‑1 (MQ=255)       tctGCAAGCTGACGCCCGTAACTCTGATCTGACCATTACCCAGCAcggcggcgg  <  1:44495/54‑1 (MQ=255)       tctGCAAGCTGACGCCCGTAACTCTGATCTGACCATTACCCAGCAcggcggcgg  <  1:50455/54‑1 (MQ=255)       tctGCAAGCTGACGCCCGTAACTCTGATCTGACCATTACCCAGCAcggcggcgg  <  1:50721/54‑1 (MQ=255)       tctGCAAGCTGACGCCCGTAACTCTGATCTGACCATTACCCAGCAcggcggcgg  <  1:50780/54‑1 (MQ=255)       tctGCAAGCTGACGCCCGTAACTCTGATCTGACCATTACCCAGCAcggcggcgg  <  1:54373/54‑1 (MQ=255)       tctGCAAGCTGACGCCCGTAACTCTGATCTGACCATTACCCAGCAcggcggcgg  <  1:55313/54‑1 (MQ=255)       tctGCAAGCTGACGCCCGTAACTCTGATCTGACCATTACCCAGCAcggcggcgg  <  1:57129/54‑1 (MQ=255)       tctGCAAGCTGACGCCCGTAACTCTGATCTGACCATTACCCAGCAcggcggcgg  <  1:5766/54‑1 (MQ=255)       tctGCAAGCTGACGCCCGTAACTCTGATCTGACCATTACCCAGCAcggcggcgg  <  1:60881/54‑1 (MQ=255)       tctGCAAGCTGACGCCCGTAACTCTGATCTGACCATTACCCAGCAcggcggcgg  <  1:61097/54‑1 (MQ=255)       tctGCAAGCTGACGCCCGTAACTCTGATCTGACCATTACCCAGCAcggcggcgg  <  1:61774/54‑1 (MQ=255)       tctGCAAGCTGACGCCCGTAACTCTGATCTGACCATTACCCAGCAcggcggcgg  <  1:66905/54‑1 (MQ=255)       tctGCAAGCTGACGCCCGTAACTCTGATCTGACCATTACCCAGCAcggcggcgg  <  1:69506/54‑1 (MQ=255)       tctGCAAGCTGACGCCCGTAACTCTGATCTGACCATTACCCAGCAcggcggcgg  <  1:70260/54‑1 (MQ=255)       tctGCAAGCTGACGCCCGTAACTCTGATCTGACCATTACCCAGCAcggcggcgg  <  1:71090/54‑1 (MQ=255)       tctGCAAGCTGACGCCCGTAACTCTGATCTGACCATTACCCAGCAcggcggcgg  <  1:72151/54‑1 (MQ=255)       tctGCAAGCTGACGCCCGTAACTCTGATCTGACCATTACCCAGCAcggcggcgg  <  1:73088/54‑1 (MQ=255)       tctGCAAGCTGACGCCCGTAACTCTGATCTGACCATTACCCAGCAcggcggcgg  <  1:74036/54‑1 (MQ=255)       tctGCAAGCTGACGCCCGTAACTCTGATCTGACCATTACCCAGCAcggcggcgg  <  1:77507/54‑1 (MQ=255)       tctGCAAGCTGACGCCCGTAACTCTGATCTGACCATTACCCAGCAcggcggcgg  <  1:77537/54‑1 (MQ=255)       tctGCAAGCTGACGCCCGTAACTCTGATCTGACCATTACCCAGCAcggcggcgg  <  1:7852/54‑1 (MQ=255)       tctGCAAGCTGACGCCCGTAACTCTGATCTGACCATTACCCAGCAcggcggcgg  <  1:79270/54‑1 (MQ=255)       tctGCAAGCTGACGCCCGTAACTCTGATCTGACCATTACCCAGCAcggcggcgg  <  1:83157/54‑1 (MQ=255)       tctGCAAGCTGACGCCCGTAACTCTGATCTGACCATTACCCAGCAcggcggcgg  <  1:84255/54‑1 (MQ=255)       tctGCAAGCTGACGCCCGTAACTCTGATCTGACCATTACCCAGCAcggcggcgg  <  1:31393/54‑1 (MQ=255)       tctGCAAGCTGACGCCCGTAACTCTGATCTGACCATTACCCAGCAcggcggcgg  <  1:13141/54‑1 (MQ=255)       tctGCAAGCTGACGCCCGTAACTCTGATCTGACCATTACCCAGCAcggcggcgg  <  1:1367/54‑1 (MQ=255)       tctGCAAGCTGACGCCCGTAACTCTGATCTGACCATTACCCAGCAcggcggcgg  <  1:14084/54‑1 (MQ=255)       tctGCAAGCTGACGCCCGTAACTCTGATCTGACCATTACCCAGCAcggcggcgg  <  1:14185/54‑1 (MQ=255)       tctGCAAGCTGACGCCCGTAACTCTGATCTGACCATTACCCAGCAcggcggcgg  <  1:17130/54‑1 (MQ=255)       tctGCAAGCTGACGCCCGTAACTCTGATCTGACCATTACCCAGCAcggcggcgg  <  1:21963/54‑1 (MQ=255)       tctGCAAGCTGACGCCCGTAACTCTGATCTGACCATTACCCAGCAcggcggcgg  <  1:22355/54‑1 (MQ=255)       tctGCAAGCTGACGCCCGTAACTCTGATCTGACCATTACCCAGCAcggcggcgg  <  1:23769/54‑1 (MQ=255)       tctGCAAGCTGACGCCCGTAACTCTGATCTGACCATTACCCAGCAcggcggcgg  <  1:24544/54‑1 (MQ=255)       tctGCAAGCTGACGCCCGTAACTCTGATCTGACCATTACCCAGCAcggcggcgg  <  1:26724/54‑1 (MQ=255)       tctGCAAGCTGACGCCCGTAACTCTGATCTGACCATTACCCAGCAcggcggcgg  <  1:30568/54‑1 (MQ=255)       tctGCAAGCTGACGCCCGTAACTCTGATCTGACCATTACCCAGCAcggcggcgg  <  1:1029/54‑1 (MQ=255)       tctGCAAGCTGACGCCCGTAACTCTGATCTGACCATTACCCAGCAcggcggcgg  <  1:33251/54‑1 (MQ=255)       tctGCAAGCTGACGCCCGTAACTCTGATCTGACCATTACCCAGCAcggcggcgg  <  1:33831/54‑1 (MQ=255)       tctGCAAGCTGACGCCCGTAACTCTGATCTGACCATTACCCAGCAcggcggcgg  <  1:36039/54‑1 (MQ=255)       tctGCAAGCTGACGCCCGTAACTCTGATCTGACCATTACCCAGCAcggcggcgg  <  1:3628/54‑1 (MQ=255)       tctGCAAGCTGACGCCCGTAACTCTGATCTGACCATTACCCAGCAcggcggcgg  <  1:37144/54‑1 (MQ=255)       tctGCAAGCTGACGCCCGTAACTCTGATCTGACCATTACCCAGCAcggcggcgg  <  1:38974/54‑1 (MQ=255)       tctGCAAGCTGACGCCCGTAACTCTGATCTGACCATTACCCAGCAcggcggcgg  <  1:40065/54‑1 (MQ=255)       tctGCAAGCTGACGCCCGTAACTCTGATCTGACCATTACCCAGCAcggcggcgg  <  1:41488/54‑1 (MQ=255)       tctGCAAGCTGACGCCCGTAACTCTGATCTGACCATTACCCAGCAcggcggcgg  <  1:41489/54‑1 (MQ=255)       tctGCAAGCTGACGCCCGTAACTCTGATCTGACCATTACCCAGCAcggcggcgg  <  1:42428/54‑1 (MQ=255)       tctGCAAGCTGACGCCCGTAACTCTGATCTGACCATTACCCAGCAcggcggcgg  <  1:42816/54‑1 (MQ=255)       tctGCAAGCTGACG‑CCGTAACTCTGATCTGACCATTACCCAGCAcggcggcgg  <  1:15312/53‑1 (MQ=255)        ctGCAAGCTGACGCCCGTAACTCTGATCTGACCATTACCCAGCAcggcggcgg  <  1:54593/53‑1 (MQ=255)                                  ||                          ACTTGCTCTGCAAACTGATGCCCGTAACTCTGACTTGACTATTACCCAGCATGGCGGCGG  >  W3110S.gb/1104855‑1104914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑