Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,528,314	2 bp→AT	coding (354‑355/618 nt)	yncG →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,528,314	0	T	A	100.0%	65.0 / NA	38	A118A (GCT→GCA)	yncG	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (38/0);  total (38/0)
*	W3110S.gb	1,528,315	0	C	T	100.0%	67.5 / NA	38	P119S (CCT→TCT)	yncG	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (38/0);  total (38/0)

TCTATCCAACATTCACTTTCGCCGATTACCCTGAGCGTTGGGCTCCTGACGCACCTGAACA  >  W3110S.gb/1528271‑1528331                                            ||                 tCTATCCAACGTTCACTTTCGCCGATTACCCTAAGCGTTGGGCATCTGACGCGCCTGAACa  >  1:9888/1‑61 (MQ=255) tCTATCCAACGTTCACTTTCGCCGATTACCCTAAGCGTTGGGCATCTGACGCACCTGAACa  >  1:52471/1‑61 (MQ=255) tCTATCCAACGTTCACTTTCGCCGATTACCCTAAGCGTTGGGCATCTGACGCACCTGAACa  >  1:52310/1‑61 (MQ=255) tCTATCCAACGTTCACTTTCGCCGATTACCCTAAGCGTTGGGCATCTGACGCACCTGAACa  >  1:53016/1‑61 (MQ=255) tCTATCCAACGTTCACTTTCGCCGATTACCCTAAGCGTTGGGCATCTGACGCACCTGAACa  >  1:53677/1‑61 (MQ=255) tCTATCCAACGTTCACTTTCGCCGATTACCCTAAGCGTTGGGCATCTGACGCACCTGAACa  >  1:53693/1‑61 (MQ=255) tCTATCCAACGTTCACTTTCGCCGATTACCCTAAGCGTTGGGCATCTGACGCACCTGAACa  >  1:56297/1‑61 (MQ=255) tCTATCCAACGTTCACTTTCGCCGATTACCCTAAGCGTTGGGCATCTGACGCACCTGAACa  >  1:58805/1‑61 (MQ=255) tCTATCCAACGTTCACTTTCGCCGATTACCCTAAGCGTTGGGCATCTGACGCACCTGAACa  >  1:62556/1‑61 (MQ=255) tCTATCCAACGTTCACTTTCGCCGATTACCCTAAGCGTTGGGCATCTGACGCACCTGAACa  >  1:63683/1‑61 (MQ=255) tCTATCCAACGTTCACTTTCGCCGATTACCCTAAGCGTTGGGCATCTGACGCACCTGAACa  >  1:65334/1‑61 (MQ=255) tCTATCCAACGTTCACTTTCGCCGATTACCCTAAGCGTTGGGCATCTGACGCACCTGAACa  >  1:65435/1‑61 (MQ=255) tCTATCCAACGTTCACTTTCGCCGATTACCCTAAGCGTTGGGCATCTGACGCACCTGAACa  >  1:70881/1‑61 (MQ=255) tCTATCCAACGTTCACTTTCGCCGATTACCCTAAGCGTTGGGCATCTGACGCACCTGAACa  >  1:74498/1‑61 (MQ=255) tCTATCCAACGTTCACTTTCGCCGATTACCCTAAGCGTTGGGCATCTGACGCACCTGAACa  >  1:78770/1‑61 (MQ=255) tCTATCCAACGTTCACTTTCGCCGATTACCCTAAGCGTTGGGCATCTGACGCACCTGAACa  >  1:8073/1‑61 (MQ=255) tCTATCCAACGTTCACTTTCGCCGATTACCCTAAGCGTTGGGCATCTGACGCACCTGAACa  >  1:86070/1‑61 (MQ=255) tCTATCCAACGTTCACTTTCGCCGATTACCCTAAGCGTTGGGCATCTGACGCACCTGAACa  >  1:86082/1‑61 (MQ=255) tCTATCCAACGTTCACTTTCGCCGATTACCCTAAGCGTTGGGCATCTGACGCACCTGAACa  >  1:87742/1‑61 (MQ=255) tCTATCCAACGTTCACTTTCGCCGATTACCCTAAGCGTTGGGCATCTGACGCACCTGAACa  >  1:34029/1‑61 (MQ=255) tCTATCCAACGTTCACTTTCGCCGATTACCCTAAGCGTTGGGCATCTGACGCACCTGAACa  >  1:15725/1‑61 (MQ=255) tCTATCCAACGTTCACTTTCGCCGATTACCCTAAGCGTTGGGCATCTGACGCACCTGAACa  >  1:16972/1‑61 (MQ=255) tCTATCCAACGTTCACTTTCGCCGATTACCCTAAGCGTTGGGCATCTGACGCACCTGAACa  >  1:17891/1‑61 (MQ=255) tCTATCCAACGTTCACTTTCGCCGATTACCCTAAGCGTTGGGCATCTGACGCACCTGAACa  >  1:21590/1‑61 (MQ=255) tCTATCCAACGTTCACTTTCGCCGATTACCCTAAGCGTTGGGCATCTGACGCACCTGAACa  >  1:22815/1‑61 (MQ=255) tCTATCCAACGTTCACTTTCGCCGATTACCCTAAGCGTTGGGCATCTGACGCACCTGAACa  >  1:23028/1‑61 (MQ=255) tCTATCCAACGTTCACTTTCGCCGATTACCCTAAGCGTTGGGCATCTGACGCACCTGAACa  >  1:24037/1‑61 (MQ=255) tCTATCCAACGTTCACTTTCGCCGATTACCCTAAGCGTTGGGCATCTGACGCACCTGAACa  >  1:24321/1‑61 (MQ=255) tCTATCCAACGTTCACTTTCGCCGATTACCCTAAGCGTTGGGCATCTGACGCACCTGAACa  >  1:296/1‑61 (MQ=255) tCTATCCAACGTTCACTTTCGCCGATTACCCTAAGCGTTGGGCATCTGACGCACCTGAACa  >  1:14939/1‑61 (MQ=255) tCTATCCAACGTTCACTTTCGCCGATTACCCTAAGCGTTGGGCATCTGACGCACCTGAACa  >  1:34438/1‑61 (MQ=255) tCTATCCAACGTTCACTTTCGCCGATTACCCTAAGCGTTGGGCATCTGACGCACCTGAACa  >  1:36718/1‑61 (MQ=255) tCTATCCAACGTTCACTTTCGCCGATTACCCTAAGCGTTGGGCATCTGACGCACCTGAACa  >  1:39259/1‑61 (MQ=255) tCTATCCAACGTTCACTTTCGCCGATTACCCTAAGCGTTGGGCATCTGACGCACCTGAACa  >  1:40721/1‑61 (MQ=255) tCTATCCAACGTTCACTTTCGCCGATTACCCTAAGCGTTGGGCATCTGACGCACCTGAACa  >  1:44815/1‑61 (MQ=255) tCTATCCAACGTTCACTTTCGCCGATTACCCTAAGCGTTGGGCATCTGACGCACCTGAACa  >  1:49917/1‑61 (MQ=255) tCTATCCAACGTTCACTTTCGCCGATTACCCTAAGCGTTGGGCATCTGACGCACCTGAACa  >  1:50193/1‑61 (MQ=255) tCTATCCAACGTTCACTTTCGCCGATTACCCTAAGCGTTGGGCATCTGACGCACCTGAACa  >  1:51467/1‑61 (MQ=255)                                            ||                 TCTATCCAACATTCACTTTCGCCGATTACCCTGAGCGTTGGGCTCCTGACGCACCTGAACA  >  W3110S.gb/1528271‑1528331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑