Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,108,409	2 bp→AT	coding (201‑202/708 nt)	yqgA →	predicted inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,108,409	0	T	A	100.0%	55.4 / NA	32	A67A (GCT→GCA)	yqgA	predicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (0/32);  total (0/32)
*	W3110S.gb	3,108,410	0	C	T	100.0%	54.2 / NA	32	L68L (CTA→TTA)	yqgA	predicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/32);  total (0/32)

TTTAGCTACGCTACTTGGCGCTCTAATTGGCGAAATTTGTTTGCTGGAGAAAGGTGTCAATA  >  W3110S.gb/3108388‑3108449                      ||                                        tttAGCAACCTTACTCGGAGCATTAATCGGCGAAATTTGTCTGCTGGAAAAAGGCGTCAATa  <  1:52669/62‑1 (MQ=255) tttAGCAACCTTACTCGGAGCATTAATCGGCGAAATTTGTCTGCTGGAAAAAGGCGTCAATa  <  1:87381/62‑1 (MQ=255) tttAGCAACCTTACTCGGAGCATTAATCGGCGAAATTTGTCTGCTGGAAAAAGGCGTCAATa  <  1:85101/62‑1 (MQ=255) tttAGCAACCTTACTCGGAGCATTAATCGGCGAAATTTGTCTGCTGGAAAAAGGCGTCAATa  <  1:81314/62‑1 (MQ=255) tttAGCAACCTTACTCGGAGCATTAATCGGCGAAATTTGTCTGCTGGAAAAAGGCGTCAATa  <  1:78499/62‑1 (MQ=255) tttAGCAACCTTACTCGGAGCATTAATCGGCGAAATTTGTCTGCTGGAAAAAGGCGTCAATa  <  1:78396/62‑1 (MQ=255) tttAGCAACCTTACTCGGAGCATTAATCGGCGAAATTTGTCTGCTGGAAAAAGGCGTCAATa  <  1:74023/62‑1 (MQ=255) tttAGCAACCTTACTCGGAGCATTAATCGGCGAAATTTGTCTGCTGGAAAAAGGCGTCAATa  <  1:72451/62‑1 (MQ=255) tttAGCAACCTTACTCGGAGCATTAATCGGCGAAATTTGTCTGCTGGAAAAAGGCGTCAATa  <  1:70830/62‑1 (MQ=255) tttAGCAACCTTACTCGGAGCATTAATCGGCGAAATTTGTCTGCTGGAAAAAGGCGTCAATa  <  1:70719/62‑1 (MQ=255) tttAGCAACCTTACTCGGAGCATTAATCGGCGAAATTTGTCTGCTGGAAAAAGGCGTCAATa  <  1:69650/62‑1 (MQ=255) tttAGCAACCTTACTCGGAGCATTAATCGGCGAAATTTGTCTGCTGGAAAAAGGCGTCAATa  <  1:58495/62‑1 (MQ=255) tttAGCAACCTTACTCGGAGCATTAATCGGCGAAATTTGTCTGCTGGAAAAAGGCGTCAATa  <  1:57210/62‑1 (MQ=255) tttAGCAACCTTACTCGGAGCATTAATCGGCGAAATTTGTCTGCTGGAAAAAGGCGTCAATa  <  1:56087/62‑1 (MQ=255) tttAGCAACCTTACTCGGAGCATTAATCGGCGAAATTTGTCTGCTGGAAAAAGGCGTCAATa  <  1:53546/62‑1 (MQ=255) tttAGCAACCTTACTCGGAGCATTAATCGGCGAAATTTGTCTGCTGGAAAAAGGCGTCAATa  <  1:53253/62‑1 (MQ=255) tttAGCAACCTTACTCGGAGCATTAATCGGCGAAATTTGTCTGCTGGAAAAAGGCGTCAATa  <  1:12352/62‑1 (MQ=255) tttAGCAACCTTACTCGGAGCATTAATCGGCGAAATTTGTCTGCTGGAAAAAGGCGTCAATa  <  1:50496/62‑1 (MQ=255) tttAGCAACCTTACTCGGAGCATTAATCGGCGAAATTTGTCTGCTGGAAAAAGGCGTCAATa  <  1:4955/62‑1 (MQ=255) tttAGCAACCTTACTCGGAGCATTAATCGGCGAAATTTGTCTGCTGGAAAAAGGCGTCAATa  <  1:47388/62‑1 (MQ=255) tttAGCAACCTTACTCGGAGCATTAATCGGCGAAATTTGTCTGCTGGAAAAAGGCGTCAATa  <  1:46125/62‑1 (MQ=255) tttAGCAACCTTACTCGGAGCATTAATCGGCGAAATTTGTCTGCTGGAAAAAGGCGTCAATa  <  1:43796/62‑1 (MQ=255) tttAGCAACCTTACTCGGAGCATTAATCGGCGAAATTTGTCTGCTGGAAAAAGGCGTCAATa  <  1:42892/62‑1 (MQ=255) tttAGCAACCTTACTCGGAGCATTAATCGGCGAAATTTGTCTGCTGGAAAAAGGCGTCAATa  <  1:40295/62‑1 (MQ=255) tttAGCAACCTTACTCGGAGCATTAATCGGCGAAATTTGTCTGCTGGAAAAAGGCGTCAATa  <  1:34887/62‑1 (MQ=255) tttAGCAACCTTACTCGGAGCATTAATCGGCGAAATTTGTCTGCTGGAAAAAGGCGTCAATa  <  1:3182/62‑1 (MQ=255) tttAGCAACCTTACTCGGAGCATTAATCGGCGAAATTTGTCTGCTGGAAAAAGGCGTCAATa  <  1:30664/62‑1 (MQ=255) tttAGCAACCTTACTCGGAGCATTAATCGGCGAAATTTGTCTGCTGGAAAAAGGCGTCAATa  <  1:29626/62‑1 (MQ=255) tttAGCAACCTTACTCGGAGCATTAATCGGCGAAATTTGTCTGCTGGAAAAAGGCGTCAATa  <  1:28554/62‑1 (MQ=255) tttAGCAACCTTACTCGGAGCATTAATCGGCGAAATTTGTCTGCTGGAAAAAGGCGTCAATa  <  1:20953/62‑1 (MQ=255) tttAGCAACCTTACTCGGAGCATTAATCGGCGAAATTTGTCTGCTGGAAAAAGGCGTCAATa  <  1:19309/62‑1 (MQ=255) tttAGCAACCTTACTCGGAGCATTAATCGGCGAAATTTGTCTGCTGGAAAAAGGCGTCAATa  <  1:13711/62‑1 (MQ=255)                      ||                                        TTTAGCTACGCTACTTGGCGCTCTAATTGGCGAAATTTGTTTGCTGGAGAAAGGTGTCAATA  >  W3110S.gb/3108388‑3108449

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑