Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,625,182	2 bp→AT	coding (423‑424/900 nt)	yigM ←	predicted inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,625,182	0	G	A	100.0%	74.2 / NA	42	L142L (CTG→TTG)	yigM	predicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/42);  total (0/42)
*	W3110S.gb	3,625,183	0	C	T	100.0%	71.1 / NA	42	L141L (TTG→TTA)	yigM	predicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/42);  total (0/42)

TGGAGAGTTGCACCAGCAGCAAGCCAGTCCAGAAATG  >  W3110S.gb/3625164‑3625200                   ||                  tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg  <  1:74139/37‑1 (MQ=25) tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg  <  1:1020/37‑1 (MQ=25) tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg  <  1:39935/37‑1 (MQ=25) tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg  <  1:49309/37‑1 (MQ=25) tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg  <  1:49432/37‑1 (MQ=25) tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg  <  1:50642/37‑1 (MQ=25) tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg  <  1:5207/37‑1 (MQ=25) tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg  <  1:69145/37‑1 (MQ=25) tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg  <  1:69510/37‑1 (MQ=25) tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg  <  1:69576/37‑1 (MQ=25) tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg  <  1:72314/37‑1 (MQ=25) tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg  <  1:35992/37‑1 (MQ=25) tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg  <  1:74359/37‑1 (MQ=25) tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg  <  1:75696/37‑1 (MQ=25) tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg  <  1:75839/37‑1 (MQ=25) tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg  <  1:76492/37‑1 (MQ=25) tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg  <  1:78778/37‑1 (MQ=25) tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg  <  1:78862/37‑1 (MQ=25) tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg  <  1:83583/37‑1 (MQ=25) tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg  <  1:86654/37‑1 (MQ=25) tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg  <  1:8782/37‑1 (MQ=25) tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg  <  1:31216/37‑1 (MQ=25) tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg  <  1:13129/37‑1 (MQ=25) tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg  <  1:14424/37‑1 (MQ=25) tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg  <  1:15003/37‑1 (MQ=25) tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg  <  1:15402/37‑1 (MQ=25) tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg  <  1:16748/37‑1 (MQ=25) tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg  <  1:18671/37‑1 (MQ=25) tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg  <  1:1966/37‑1 (MQ=25) tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg  <  1:19667/37‑1 (MQ=25) tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg  <  1:22307/37‑1 (MQ=25) tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg  <  1:23113/37‑1 (MQ=25) tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg  <  1:23434/37‑1 (MQ=25) tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg  <  1:26025/37‑1 (MQ=25) tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg  <  1:26699/37‑1 (MQ=25) tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg  <  1:30421/37‑1 (MQ=25) tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg  <  1:30618/37‑1 (MQ=25) tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg  <  1:3353/37‑1 (MQ=25) tGGAGAGTTGCACCAGCAATAAGCAAGTCCAGAAATg  <  1:1670/37‑1 (MQ=21)  ggAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg  <  1:15534/36‑1 (MQ=25)  ggAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg  <  1:46241/36‑1 (MQ=25)  ggAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg  <  1:10787/36‑1 (MQ=25)                   ||                  TGGAGAGTTGCACCAGCAGCAAGCCAGTCCAGAAATG  >  W3110S.gb/3625164‑3625200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑