Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,686,419	2 bp→CG	intergenic (‑58/‑264)	ilvL ← / → yifB	ilv operon leader peptide/predicted bifunctional protein, enzyme and transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,686,419	0	T	C	100.0%	112.5 / NA	61	intergenic (‑58/‑265)	ilvL/yifB	ilv operon leader peptide/predicted bifunctional protein, enzyme and transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/61);  total (0/61)
*	W3110S.gb	3,686,420	0	A	G	100.0%	116.9 / NA	61	intergenic (‑59/‑264)	ilvL/yifB	ilv operon leader peptide/predicted bifunctional protein, enzyme and transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/61);  total (0/61)

ATGCCTAAAGAGTTACCATAGGTTTTGTAAATAGTACAAGATATTT  >  W3110S.gb/3686392‑3686437                            ||                  aTGCCTAAATAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:86047/46‑1 (MQ=25) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACTAGATAttt  <  1:52357/46‑1 (MQ=255) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:73284/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:58832/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:5944/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:59752/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:63090/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:63543/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:64686/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:65454/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:6628/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:67243/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:67769/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:6788/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:70572/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:71714/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:71959/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:57500/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:75265/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:76079/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:76692/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:79562/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:79805/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:82260/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:83351/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:85020/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:86947/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:87227/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:8725/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:88032/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:9382/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:33893/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:15999/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:17001/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:19936/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:20934/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:21701/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:22135/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:22586/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:22696/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:23717/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:23803/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:24530/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:27234/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:31290/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:11006/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:39591/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:39713/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:39925/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:42950/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:44029/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:44963/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:51965/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:5217/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:52863/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:52886/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:53167/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:54472/46‑1 (MQ=37) aTGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:54692/46‑1 (MQ=37)  tGCCTAAAGAGTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:80933/45‑1 (MQ=25)         agagTTACCATAGATTTTGCGAATAGCACAAGATAttt  <  1:41408/38‑1 (MQ=21)                            ||                  ATGCCTAAAGAGTTACCATAGGTTTTGTAAATAGTACAAGATATTT  >  W3110S.gb/3686392‑3686437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑