Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,171,558	2 bp→AG	coding (994‑995/2694 nt)	chiA →	periplasmic endochitinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,171,558	0	G	A	100.0%	130.1 / NA	70	V332S (GTT→AGT)	chiA	periplasmic endochitinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/70);  total (0/70)
*	W3110S.gb	4,171,559	0	T	G	100.0%	136.0 / NA	70	V332S (GTT→AGT)	chiA	periplasmic endochitinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (0/70);  total (0/70)

AGCGAGCCATCAACACCAGCGGATAGCGTTAACGATTACTCATTGCAAGCGTGGAGCGGCC  >  W3110S.gb/4171531‑4171591                            ||                                 aacGAGCCATCAAGACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:79209/59‑1 (MQ=255) aacGAGCCATCAACACTAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:29845/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCATGCGTGGAGTGGcc  <  1:45325/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:71043/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:72060/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:70439/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:63918/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:63854/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:63517/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:61318/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:60103/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:56439/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:54686/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:54497/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:5074/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:49422/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:48815/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:1101/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:45607/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:72144/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:73208/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:73680/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:75535/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:76257/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:77394/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:77661/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:7894/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:7929/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:79546/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:80825/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:81036/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:82789/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:8424/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:86212/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:14526/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:45487/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:26006/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:25512/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:24165/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:23015/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:18792/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:16668/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:15693/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:26411/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:14034/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:13466/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:13041/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:12922/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:12641/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:11613/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:11296/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:32570/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:43348/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:4236/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:41841/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:40268/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:37766/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:32621/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:2855/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:31796/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:30832/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:30658/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:30256/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:29810/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:29094/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGGGGAGTGGcc  <  1:41005/59‑1 (MQ=255) aacGAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACCGCAGGCGTGGAGTGGcc  <  1:564/59‑1 (MQ=255)    gAGCCATCAACACCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:76575/58‑1 (MQ=255)             acacCAGCGGATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:83809/49‑1 (MQ=255)                      gATAGCAGTAACGATTACTCACTGCAGGCGTGGAGTGGcc  <  1:71331/40‑1 (MQ=255)                            ||                                 AGCGAGCCATCAACACCAGCGGATAGCGTTAACGATTACTCATTGCAAGCGTGGAGCGGCC  >  W3110S.gb/4171531‑4171591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑