Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 4,248,181 3 bp→CGC coding (485‑487/1545 nt) malF ← maltose transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,248,1810TC100.0% 68.6 / NA 39T163A (ACC→GCC) malFmaltose transporter subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/39);  total (0/39)
*W3110S.gb4,248,1820CG100.0% 72.5 / NA 39T162S (ACG→AGCmalFmaltose transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (0/39);  total (0/39)
*W3110S.gb4,248,1830GC100.0% 69.7 / NA 39T162S (ACG→AGC) malFmaltose transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (0/39);  total (0/39)

CGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAA  >  W3110S.gb/4248155‑4248217
                          |||                                  
cgcgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTCAGTTTTTGCTCGCCGCCaaa  <  1:42089/62‑1 (MQ=255)
  cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:63965/61‑1 (MQ=255)
  cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:49304/61‑1 (MQ=255)
  cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:56452/61‑1 (MQ=255)
  cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:56849/61‑1 (MQ=255)
  cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:60624/61‑1 (MQ=255)
  cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:6119/61‑1 (MQ=255)
  cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:6132/61‑1 (MQ=255)
  cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:61332/61‑1 (MQ=255)
  cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:62071/61‑1 (MQ=255)
  cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:44876/61‑1 (MQ=255)
  cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:66167/61‑1 (MQ=255)
  cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:67795/61‑1 (MQ=255)
  cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:69728/61‑1 (MQ=255)
  cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:71941/61‑1 (MQ=255)
  cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:74073/61‑1 (MQ=255)
  cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:74749/61‑1 (MQ=255)
  cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:75731/61‑1 (MQ=255)
  cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:81697/61‑1 (MQ=255)
  cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:82675/61‑1 (MQ=255)
  cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:28501/61‑1 (MQ=255)
  cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:12601/61‑1 (MQ=255)
  cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:14687/61‑1 (MQ=255)
  cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:17421/61‑1 (MQ=255)
  cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:17632/61‑1 (MQ=255)
  cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:21401/61‑1 (MQ=255)
  cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:22060/61‑1 (MQ=255)
  cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:26316/61‑1 (MQ=255)
  cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:27323/61‑1 (MQ=255)
  cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:27969/61‑1 (MQ=255)
  cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:48026/61‑1 (MQ=255)
  cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:32096/61‑1 (MQ=255)
  cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:33229/61‑1 (MQ=255)
  cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:35515/61‑1 (MQ=255)
  cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:38745/61‑1 (MQ=255)
  cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:39120/61‑1 (MQ=255)
  cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:42155/61‑1 (MQ=255)
  cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:42337/61‑1 (MQ=255)
  cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:10/61‑1 (MQ=255)
                          |||                                  
CGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAA  >  W3110S.gb/4248155‑4248217

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: