Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,456,713	2 bp→CG	coding (974‑975/1503 nt)	ytfR →	predicted sugar transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,456,713	0	A	C	100.0%	71.0 / NA	38	N325T (AAC→ACG)	ytfR	predicted sugar transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (38/0);  total (38/0)
*	W3110S.gb	4,456,714	0	C	G	100.0%	67.2 / NA	38	N325T (AAC→ACG)	ytfR	predicted sugar transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (38/0);  total (38/0)

GCGTTGATCAAAGGCAAACCGCAAAACCTGCGATCGCCACATCAGGCTTCG  >  W3110S.gb/4456688‑4456738                          ||                         gCGTTGATCAAAGGCAAAGCGCAAACGCTACGCTCGCCACATCAGGCTTCg  >  1:9093/1‑51 (MQ=255) gCGTTGATCAAAGGCAAACCGCAAACGCTACGCTCGCCACATCAGGCTTc   >  1:40343/1‑50 (MQ=255) gCGTTGATCAAAGGCAAACCGCAAACGCTACGCTCGCCACATCAGGCTTCg  >  1:66947/1‑51 (MQ=255) gCGTTGATCAAAGGCAAACCGCAAACGCTACGCTCGCCACATCAGGCTTCg  >  1:5813/1‑51 (MQ=255) gCGTTGATCAAAGGCAAACCGCAAACGCTACGCTCGCCACATCAGGCTTCg  >  1:60075/1‑51 (MQ=255) gCGTTGATCAAAGGCAAACCGCAAACGCTACGCTCGCCACATCAGGCTTCg  >  1:60443/1‑51 (MQ=255) gCGTTGATCAAAGGCAAACCGCAAACGCTACGCTCGCCACATCAGGCTTCg  >  1:61186/1‑51 (MQ=255) gCGTTGATCAAAGGCAAACCGCAAACGCTACGCTCGCCACATCAGGCTTCg  >  1:63050/1‑51 (MQ=255) gCGTTGATCAAAGGCAAACCGCAAACGCTACGCTCGCCACATCAGGCTTCg  >  1:63202/1‑51 (MQ=255) gCGTTGATCAAAGGCAAACCGCAAACGCTACGCTCGCCACATCAGGCTTCg  >  1:63349/1‑51 (MQ=255) gCGTTGATCAAAGGCAAACCGCAAACGCTACGCTCGCCACATCAGGCTTCg  >  1:65015/1‑51 (MQ=255) gCGTTGATCAAAGGCAAACCGCAAACGCTACGCTCGCCACATCAGGCTTCg  >  1:53466/1‑51 (MQ=255) gCGTTGATCAAAGGCAAACCGCAAACGCTACGCTCGCCACATCAGGCTTCg  >  1:69614/1‑51 (MQ=255) gCGTTGATCAAAGGCAAACCGCAAACGCTACGCTCGCCACATCAGGCTTCg  >  1:70565/1‑51 (MQ=255) gCGTTGATCAAAGGCAAACCGCAAACGCTACGCTCGCCACATCAGGCTTCg  >  1:75878/1‑51 (MQ=255) gCGTTGATCAAAGGCAAACCGCAAACGCTACGCTCGCCACATCAGGCTTCg  >  1:83446/1‑51 (MQ=255) gCGTTGATCAAAGGCAAACCGCAAACGCTACGCTCGCCACATCAGGCTTCg  >  1:87000/1‑51 (MQ=255) gCGTTGATCAAAGGCAAACCGCAAACGCTACGCTCGCCACATCAGGCTTCg  >  1:8748/1‑51 (MQ=255) gCGTTGATCAAAGGCAAACCGCAAACGCTACGCTCGCCACATCAGGCTTCg  >  1:8804/1‑51 (MQ=255) gCGTTGATCAAAGGCAAACCGCAAACGCTACGCTCGCCACATCAGGCTTCg  >  1:9179/1‑51 (MQ=255) gCGTTGATCAAAGGCAAACCGCAAACGCTACGCTCGCCACATCAGGCTTCg  >  1:31565/1‑51 (MQ=255) gCGTTGATCAAAGGCAAACCGCAAACGCTACGCTCGCCACATCAGGCTTCg  >  1:14557/1‑51 (MQ=255) gCGTTGATCAAAGGCAAACCGCAAACGCTACGCTCGCCACATCAGGCTTCg  >  1:16252/1‑51 (MQ=255) gCGTTGATCAAAGGCAAACCGCAAACGCTACGCTCGCCACATCAGGCTTCg  >  1:16511/1‑51 (MQ=255) gCGTTGATCAAAGGCAAACCGCAAACGCTACGCTCGCCACATCAGGCTTCg  >  1:21346/1‑51 (MQ=255) gCGTTGATCAAAGGCAAACCGCAAACGCTACGCTCGCCACATCAGGCTTCg  >  1:28371/1‑51 (MQ=255) gCGTTGATCAAAGGCAAACCGCAAACGCTACGCTCGCCACATCAGGCTTCg  >  1:28650/1‑51 (MQ=255) gCGTTGATCAAAGGCAAACCGCAAACGCTACGCTCGCCACATCAGGCTTCg  >  1:28688/1‑51 (MQ=255) gCGTTGATCAAAGGCAAACCGCAAACGCTACGCTCGCCACATCAGGCTTCg  >  1:29931/1‑51 (MQ=255) gCGTTGATCAAAGGCAAACCGCAAACGCTACGCTCGCCACATCAGGCTTCg  >  1:12381/1‑51 (MQ=255) gCGTTGATCAAAGGCAAACCGCAAACGCTACGCTCGCCACATCAGGCTTCg  >  1:34398/1‑51 (MQ=255) gCGTTGATCAAAGGCAAACCGCAAACGCTACGCTCGCCACATCAGGCTTCg  >  1:35251/1‑51 (MQ=255) gCGTTGATCAAAGGCAAACCGCAAACGCTACGCTCGCCACATCAGGCTTCg  >  1:35587/1‑51 (MQ=255) gCGTTGATCAAAGGCAAACCGCAAACGCTACGCTCGCCACATCAGGCTTCg  >  1:39936/1‑51 (MQ=255) gCGTTGATCAAAGGCAAACCGCAAACGCTACGCTCGCCACATCAGGCTTCg  >  1:45985/1‑51 (MQ=255) gCGTTGATCAAAGGCAAACCGCAAACGCTACGCTCGCCACATCAGGCTTCg  >  1:50740/1‑51 (MQ=255) gCGTTGATCAAAGGCAAACCGCAAACGCTACGCTCGCCACATCAGGCTTCg  >  1:50788/1‑51 (MQ=255) gCGTTGATCAAAGGCAAACCGCAAACGCTACGCTCGCCACATCAGGCTTCg  >  1:51078/1‑51 (MQ=255)                          ||                         GCGTTGATCAAAGGCAAACCGCAAAACCTGCGATCGCCACATCAGGCTTCG  >  W3110S.gb/4456688‑4456738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑