Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 4,371,276 (A)5→3 intergenic (‑9/+296) dcuA ← / ← aspA C4‑dicarboxylate antiporter/aspartate ammonia‑lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,371,2730A.100.0% 159.8 / NA 37intergenic (‑6/+300)dcuA/aspAC4‑dicarboxylate antiporter/aspartate ammonia‑lyase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (25/12);  total (25/12)
*W3110S.gb4,371,2740A.100.0% 159.8 / NA 37intergenic (‑7/+299)dcuA/aspAC4‑dicarboxylate antiporter/aspartate ammonia‑lyase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (25/12);  total (25/12)

CTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAA  >  W3110S.gb/4371225‑4371317
                                                ||                                           
ctgtctgATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGa                                 <  1:651422/60‑1 (MQ=255)
    ctgATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAAcc                            >  1:3236289/1‑61 (MQ=255)
    ctgATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAAcc                            >  1:1289775/1‑61 (MQ=255)
    ctgATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAAcc                            >  1:809352/1‑61 (MQ=255)
    ctgATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAAcc                            >  1:1487466/1‑61 (MQ=255)
    ctgATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAAcc                            >  1:666056/1‑61 (MQ=255)
    ctgATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAAcc                            >  1:2399831/1‑61 (MQ=255)
    ctgATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAAcc                            >  1:1704804/1‑61 (MQ=255)
    ctgATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAAcc                            >  1:2391716/1‑61 (MQ=255)
    ctgATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAAcc                            >  1:701913/1‑61 (MQ=255)
          aaCCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATg                      >  1:2267332/1‑61 (MQ=255)
          aaCCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGa                     <  1:3644609/62‑1 (MQ=255)
          aaCCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGa                     <  1:1246507/62‑1 (MQ=255)
                aTAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATaa                  >  1:1148221/1‑59 (MQ=255)
                aTAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATaa                  >  1:1614898/1‑59 (MQ=255)
                aTAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATaa                  >  1:702441/1‑59 (MQ=255)
                aTAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATaa                  >  1:1153679/1‑59 (MQ=255)
                     ccAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATg          <  1:2309640/62‑1 (MQ=255)
                     ccAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATg          <  1:3851744/62‑1 (MQ=255)
                       aGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATg          <  1:338059/60‑1 (MQ=255)
                        gACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGAc        >  1:1792971/1‑61 (MQ=255)
                        gACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGAc        >  1:1070297/1‑61 (MQ=255)
                        gACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGAc        >  1:3915846/1‑61 (MQ=255)
                        gACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACa       >  1:2291915/1‑62 (MQ=255)
                        gACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACa       >  1:4270385/1‑62 (MQ=255)
                         aCCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACAt      <  1:434302/62‑1 (MQ=255)
                         aCCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACAt      <  1:833982/62‑1 (MQ=255)
                         aCCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACAt      <  1:3417825/62‑1 (MQ=255)
                         aCCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACAt      <  1:2005903/62‑1 (MQ=255)
                         aCCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACAt      <  1:1599963/62‑1 (MQ=255)
                         aCCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACAt      <  1:1482183/62‑1 (MQ=255)
                          ccGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAAcc                            >  1:2714166/1‑39 (MQ=37)
                             ccAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGaa  >  1:3791299/1‑62 (MQ=255)
                             ccAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGaa  >  1:3729362/1‑62 (MQ=255)
                             ccAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGaa  >  1:4675882/1‑62 (MQ=255)
                             ccAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGaa  >  1:2864085/1‑62 (MQ=255)
                             ccAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGaa  >  1:1244499/1‑62 (MQ=255)
                                                ||                                           
CTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAA  >  W3110S.gb/4371225‑4371317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑