Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	453,398	+G	intergenic (‑7/‑298)	yajG ← / → bolA	predicted lipoprotein/regulator of penicillin binding proteins and beta lactamase transcription (morphogene)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	453,395	1	.	G	100.0%	27.4 / NA	9	intergenic (‑4/‑301)	yajG/bolA	predicted lipoprotein/regulator of penicillin binding proteins and beta lactamase transcription (morphogene)
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (3/6);  total (3/6)

CATCCTGCAAGCATAAACAGAGCAACTAACGGGAAGAGGATTTTTTTGAACATGTTC‑GGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATTCCCGCCATCATAA  >  W3110S.gb/453339‑453446                                                          |                                                    cATCCTGCAAGCATAAACAGAGCAACTAACGGGAAGAGGATTTTTTTGAACATGTTC‑ggggc                                                <  1:172126/62‑3 (MQ=255) cATCCTGCAAGCATAAACAGAGCAACTAACGGGAAGAGGATTTTTTTGAACATGTTC‑ggggc                                                <  1:3396603/62‑3 (MQ=255)               aaaCAGAGCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGGCTCTCAGAGACTCtt                                   <  1:312947/62‑1 (MQ=255)               aaaCAGAGCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGGCTCTCAGAGACTCtt                                   <  1:3426515/62‑1 (MQ=255)                                      ggATTTTTTTGAACATGTTCGGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTaaaa                  >  1:2239670/1‑56 (MQ=255)                                      ggATTTTTTTGAACATGTTCGGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATTccc            >  1:2461804/1‑62 (MQ=255)                                      ggATTTTTTTGAACATGTTCGGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATTccc            >  1:2875183/1‑62 (MQ=255)                                         tttttttGAACATGTTCGGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATTCCCGcc         <  1:805194/62‑1 (MQ=255)                                         tttttttGAACATGTTCGGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATTCCCGcc         <  1:942873/62‑1 (MQ=255)                                           tttttGAACATGTTCGGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAAt                <  1:2918818/53‑1 (MQ=255)                                                 aaCATGTTCGGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATTCCCGCCATCATaa  <  1:4035113/61‑1 (MQ=255)                                                          |                                                    CATCCTGCAAGCATAAACAGAGCAACTAACGGGAAGAGGATTTTTTTGAACATGTTC‑GGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATTCCCGCCATCATAA  >  W3110S.gb/453339‑453446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑