| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 547,835 | +G | pseudogene (255/261 nt) pseudogene (255/1259 nt) |
ylbE → ylbE → |
ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment ECK0512:JW0508+JW0507:b4572; hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 547,831 | 1 | . | G | 97.2% | 128.2 / ‑3.1 | 36 | pseudogene (251/261 nt) pseudogene (251/1259 nt) | ylbE ylbE | ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment ECK0512:JW0508+JW0507:b4572; hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (24/11); total (25/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
GCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑GGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGG > W3110S.gb/547773‑547887 | gcATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑ggg < 1:3658952/62‑1 (MQ=255)gcATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑ggg < 1:2460653/62‑1 (MQ=255)gcATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑ggg < 1:4321430/62‑1 (MQ=255)gcATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑ggg < 1:2172386/62‑1 (MQ=255)gcATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑ggg < 1:3158120/62‑1 (MQ=255)gcATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑ggg < 1:1079776/62‑1 (MQ=255)gcATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑ggg < 1:1636962/62‑1 (MQ=255)gcATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑ggg < 1:1380509/62‑1 (MQ=255)gcATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑ggg < 1:841724/62‑1 (MQ=255)gcATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑ggg < 1:3789608/62‑1 (MQ=255) cATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑gggg < 1:4576502/62‑1 (MQ=255) cATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑gggg < 1:4535208/62‑1 (MQ=255) cATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑gggg < 1:226640/62‑1 (MQ=255) cATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑gggg < 1:2376275/62‑1 (MQ=255) cATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑gggg < 1:449364/62‑1 (MQ=255) tGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGG‑AAGTg > 1:2543428/1‑62 (MQ=255) gAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGG‑AAGTGAACtt > 1:3931158/1‑62 (MQ=255) gAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGG‑AAGTGAACtt > 1:727754/1‑62 (MQ=255) gAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGG‑AAGTGAACtt > 1:4031169/1‑62 (MQ=255) gAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGG‑AAGTGAACtt > 1:1205238/1‑62 (MQ=255) gAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGG‑AAGTGAACt > 1:3446511/1‑61 (MQ=255) gAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGG‑AAGTGAACt > 1:844200/1‑61 (MQ=255) aaaGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGG‑AAGTGAACTTCATTCCTTGTCa < 1:2045817/62‑1 (MQ=255) aaGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGG‑AAGTGaa > 1:2046019/1‑46 (MQ=38) aaGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGG‑AAGTGAACTTCATTCCTTGTCa > 1:1261892/1‑61 (MQ=255) aaGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGG‑AAGTGAACTTCATTCCTTGTCAc > 1:4151559/1‑62 (MQ=255) aaGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGG‑AAGTGAACTTCATTCCTTGTCAc > 1:2240112/1‑62 (MQ=255) aaGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGG‑AAGTGAACTTCATTCCTTGTCAc > 1:1560790/1‑62 (MQ=255) aaGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGG‑AAGTGAACTTCATTCCTTGTCAc > 1:3895682/1‑62 (MQ=255) aaGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGG‑AAGTGAACTTCATTCCTTGTCAc > 1:1320215/1‑62 (MQ=255) gAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGG‑AAGTGAACTTCATTCCTTGTCACCAtg > 1:3328202/1‑61 (MQ=255) gAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGG‑AAGTGAACTTCATTCCTTGTCACCAtg > 1:3437347/1‑61 (MQ=255) gAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGG‑AAGTGAACTTCATTCCTTGTCACCAtg > 1:1835032/1‑61 (MQ=255) gAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGG‑AAGTGAACTTCATTCCTTGTCACCAtg > 1:2153426/1‑61 (MQ=255) gAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGG‑AAGTGAACTTCATTCCTTGTCACCAtg > 1:2504137/1‑61 (MQ=255) gAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGG‑AAGTGAACTTCATTCCTTGTCACCAtg > 1:2409042/1‑61 (MQ=255) ggCGCTGGCAATACTGGAGCAGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCg < 1:443585/62‑1 (MQ=255) cTGGCAATACTGGAGCAGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCgg > 1:421106/1‑62 (MQ=255) cTGGCAATACTGGAGCAGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCgg > 1:2412805/1‑62 (MQ=255) aaTACTGGAGCAGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCaa < 1:3574527/62‑1 (MQ=255) aaTACTGGAGCAGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCaa < 1:1342631/62‑1 (MQ=255) aCTGGAGCAGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCg < 1:3364215/50‑1 (MQ=255) aCTGGAGCAGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATgg < 1:1080931/62‑1 (MQ=255) aCTGGAGCAGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATgg < 1:4023656/62‑1 (MQ=255) aCTGGAGCAGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATgg < 1:396140/62‑1 (MQ=255) cTGGAGCAGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATggg > 1:670669/1‑62 (MQ=255) cTGGAGCAGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATggg > 1:171950/1‑62 (MQ=255) ggAGCAGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCg < 1:3036903/62‑1 (MQ=255) ggAGCAGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCg < 1:739427/62‑1 (MQ=255) ggAGCAGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCg < 1:3792904/62‑1 (MQ=255) aGCA‑GGGGTAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCgg > 1:752618/1‑61 (MQ=255) | GCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑GGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGG > W3110S.gb/547773‑547887 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |