Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,394,475	(A)8→9	coding (771/900 nt)	ycjZ →	predicted DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,394,467	1	.	A	100.0%	62.4 / NA	20	E255K (GAA→AAA)	ycjZ	predicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (7/13);  total (7/13)

ATTGATGGGCATGGATTGGCGTATCTACCTTATGATCAGGTTGAGCGGGCTATTAAAG‑AAAAAAAACTGATACGTGTTTTGGATAAATTCACACCAGATTTACCC  >  W3110S.gb/1394410‑1394514                                                           |                                                aTTGATGGGCATGGATTGGCGTATCTACCTTATGATCAGGTTGAGCGGGCTATTAAAG‑aaaa                                             <  1:617332/62‑1 (MQ=255) aTTGATGGGCATGGATTGGCGTATCTACCTTATGATCAGGTTGAGCGGGCTATTAAAG‑aaaa                                             <  1:4186931/62‑1 (MQ=255) aTTGATGGGCATGGATTGGCGTATCTACCTTATGATCAGGTTGAGCGGGCTATTAAAG‑aaaa                                             <  1:1684966/62‑1 (MQ=255) aTTGATGGGCATGGATTGGCGTATCTACCTTATGATCAGGTTGAGCGGGCTATTAAAG‑aaaa                                             <  1:908442/62‑1 (MQ=255)   tGATGGGCTTGGATTGGCGTATCTACCTTATGATCAGGTTGAGCGGGCTATTAAAG‑aaaaaa                                           <  1:2375092/62‑1 (MQ=255)   tGATGGGCATGGATTGGCGTATCTACCTTATGATCAGGTTGAGCGGGCTATTAAAG‑aaaaaa                                           <  1:2926224/62‑1 (MQ=255)   tGATGGGCATGGATTGGCGTATCTACCTTATGATCAGGTTGAGCGGGCTATTAAAG‑aaaaaa                                           <  1:4060460/62‑1 (MQ=255)   tGATGGGCATGGATTGGCGTATCTACCTTATGATCAGGTTGAGCGGGCTATTAAAG‑aaaaaa                                           <  1:3729558/62‑1 (MQ=255)   tGATGGGCATGGATTGGCGTATCTACCTTATGATCAGGTTGAGCGGGCTATTAAAG‑aaaaaa                                           <  1:1653431/62‑1 (MQ=255)    gATGGGCATGGATTGGCGTATCTACCTTATGATCAGGTTGAGCGGGCTATTAAAG‑a                                                <  1:3007117/56‑1 (MQ=255)                    cGTATCTACCTTATGATCAGGTTGAGCGGGCTATTAAAGAAAAAAAAACTGATACGTGttt                            <  1:997669/61‑1 (MQ=255)                    cGTATCTACCTTATGATCAGGTTGAGCGGGCTATTAAAGAAAAAAAAACTGATACGTGttt                            <  1:1481798/61‑1 (MQ=255)                    cGTATCTACCTTATGATCAGGTTGAGCGGGCTATTAAAGAAAAAAAAACTGATACGTGttt                            <  1:3983037/61‑1 (MQ=255)                    cGTATCTACCTTATGATCAGGTTGAGCGGGCTATTAAAGAAAAAAAAACTGATACGTGttt                            <  1:831088/61‑1 (MQ=255)                               tATGATCAGGTTGAGCGGGCTATTAAAGAAAAAAAAACTGATACGTGTTTTGGATAAATTca                >  1:1270935/1‑62 (MQ=255)                               tATGATCAGGTTGAGCGGGCTATTAAAGAAAAAAAAACTGATACGTGTTTTGGATAAATTca                >  1:2861979/1‑62 (MQ=255)                               tATGATCAGGTTGAGCGGGCTATTAAAGAAAAAAAAACTGATACGTGTTTTGGATAAATTca                >  1:4051197/1‑62 (MQ=255)                               tATGATCAGGTTGAGCGGGCTATTAAAGAAAAAAAAACTGATACGTGTTTTGGATAAATTca                >  1:1580566/1‑62 (MQ=255)                                 tGATCAGGTTGAGCGGGCTATTAAAGAAAAAAAAACTGATACGTGTTTTGGATAAATTcac               >  1:2692312/1‑61 (MQ=255)                                 tGATCAGGTTGAGCGGGCTATTAAAGAAAAAAAAACTGATACGTGTTTTGGATAAATTcac               >  1:2379823/1‑61 (MQ=255)                                  gATCAGGTTGAGCGGGCTATTAAAGAAAAAAAAACTGATACGTGTTTTGGATAAATTcac               <  1:1843321/60‑1 (MQ=255)                                  gATCAGGTTGAGCGGGCTATTAAAGAAAAAAAAACTGATACGTGTTTTGGATAAATTcac               <  1:4060499/60‑1 (MQ=255)                                  gATCAGGTTGAGCGGGCTATTAAAGAAAAAAAAACTGATACGTGTTTTGGATAAATTcac               <  1:2047422/60‑1 (MQ=255)                                  gATCAGGTTGAGCGGGCTATTAAAGAAAAAAAAACTGATACGTGTTTTGGATAAATTcac               <  1:4391848/60‑1 (MQ=255)                                  gATCAGGTTGAGCGGGCTATTAAAGAAAAAAAAACTGATACGTGTTTTGGATAAATTcac               <  1:2130440/60‑1 (MQ=255)                                       ggTTGAGCGGGCTATTAAAGAAAAAAAAACTGATACGTGTTTTGGATAAATTCACACCAGa         >  1:3404761/1‑61 (MQ=255)                                           gAGCGGGCTATTAAAGAAAAAAAAACTGATACGTCttt                            <  1:3763111/38‑1 (MQ=25)                                             gCGGGCTATTAAAGAAAAAAAAACTGATACGTGTTTTGGATAAATTCACACCAGATTTAcc   <  1:4399271/61‑1 (MQ=255)                                              cGGGCTATTAAAGAAAAAAAAACTGATACGTGTTTTGGATAAATTCACACCAGATTTAccc  <  1:3619547/61‑1 (MQ=255)                                               gggCTATTAAAGAAAAAAAAACTGATACGTGTTTTGGATAAATTCACACCAGATTTAccc  <  1:1293320/60‑1 (MQ=255)                                                           |                                                ATTGATGGGCATGGATTGGCGTATCTACCTTATGATCAGGTTGAGCGGGCTATTAAAG‑AAAAAAAACTGATACGTGTTTTGGATAAATTCACACCAGATTTACCC  >  W3110S.gb/1394410‑1394514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑