Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,454,872	+C	coding (485/2046 nt)	maoC ←	fused aldehyde dehydrogenase and enoyl‑CoA hydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,454,868	1	.	C	100.0%	83.4 / NA	24	G163G (GGA→GGG)	maoC	fused aldehyde dehydrogenase and enoyl‑CoA hydratase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (5/19);  total (5/19)

TGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATT‑CCCCAGCAGGGGAAGTTAAAGGCGTTAATATGCACTGC  >  W3110S.gb/1454819‑1454906                                                   |                                       tgatgGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATTCCCCcagcag                               >  1:2772388/1‑60 (MQ=255) tgatgGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATTCCCCCAGCAgg                              >  1:4264148/1‑61 (MQ=255) tgatgGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATTCCCCCAGCAgg                              >  1:3258022/1‑61 (MQ=255) tgatgGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATTCCCCCAGCAgg                              >  1:1992459/1‑61 (MQ=255) tgatgGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATTCCCCCAGCAgg                              >  1:2562504/1‑61 (MQ=255)                     aGCCACGTTGGTGCCAGCTTTTCCAGCATTCCCCCAGCAGGGGAAGTTAAAGGCGTTAatat         <  1:3260128/62‑1 (MQ=255)                        cACGTTGGTGCCAGCTTTTCCAGCATTCCCCCAGCAggg                             <  1:579006/39‑1 (MQ=37)                        cACGTTGGTGCCAGCTTTTCCAGCATTCCCCCAGCAggg                             <  1:574646/39‑1 (MQ=37)                        cACGTTGGTGCCAGCTTTTCCAGCATTCCCCCAGCAggg                             <  1:3731301/39‑1 (MQ=37)                        cACGTTGGTGCCAGCTTTTCCAGCATTCCCCCAGCAggg                             <  1:312396/39‑1 (MQ=37)                        cACGTTGGTGCCAGCTTTTCCAGCATTCCCCCAGCAggg                             <  1:2475288/39‑1 (MQ=37)                        cACGTTGGTGCCAGCTTTTCCAGCATTCCCCCAGCAggg                             <  1:2355299/39‑1 (MQ=37)                        cACGTTGGTGCCAGCTTTTCCAGCATTCCCCCAGCAggg                             <  1:1957168/39‑1 (MQ=37)                        cACGTTGGTGCCAGCTTTTCCAGCATTCCCCCAGCAggg                             <  1:1822689/39‑1 (MQ=37)                        cACGTTGGTGCCAGCTTTTCCAGCATTCCCCCAGCAggg                             <  1:1622349/39‑1 (MQ=37)                        cACGTTGGTGCCAGCTTTTCCAGCATTCCCCCAGCAggg                             <  1:1399378/39‑1 (MQ=37)                        cACGTTGGTGCCAGCTTTTCCAGCATTCCCCCAGCAggg                             <  1:1370445/39‑1 (MQ=37)                        cACGTTGGTGCCAGCTTTTCCAGCATTCCCCCAGCAggg                             <  1:1178859/39‑1 (MQ=37)                            ttGGTGCCAGCTTTTCCAGCATTCCCCCAGCAGGGGAAGTTAAAGGCGTTAATATGCACTGc  <  1:2258312/62‑1 (MQ=255)                            ttGGTGCCAGCTTTTCCAGCATTCCCCCAGCAGGGGAAGTTAAAGGCGTTAATATGCACTGc  <  1:1919939/62‑1 (MQ=255)                            ttGGTGCCAGCTTTTCCAGCATTCCCCCAGCAGGGGAAGTTAAAGGCGTTAATATGCACTGc  <  1:3321329/62‑1 (MQ=255)                            ttGGTGCCAGCTTTTCCAGCATTCCCCCAGCAGGGGAAGTTAAAGGCGTTAATATGCACTGc  <  1:3772801/62‑1 (MQ=255)                            ttGGTGCCAGCTTTTCCAGCATTCCCCCAGCAGGGGAAGTTAAAGGCGTTAATATGCACTGc  <  1:4185781/62‑1 (MQ=255)                            ttGGTGCCAGCTTTTCCAGCATTCCCCCAGCAGGGGAAGTTAAAGGCGTTAATATGCACTGc  <  1:1164887/62‑1 (MQ=255)                                                   |                                       TGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATT‑CCCCAGCAGGGGAAGTTAAAGGCGTTAATATGCACTGC  >  W3110S.gb/1454819‑1454906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑