Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,891,326	(C)5→6	coding (135/1686 nt)	fadD ←	acyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,891,321	1	.	C	75.0%	14.9 / 1.8	8	coding (140/1686 nt)	fadD	acyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base . (0/2);  new base C (6/0);  total (6/2)
Fisher's exact test for biased strand distribution p-value = 3.57e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.72e-01

TTGCAAATAAGCGGCAAACGCGCGACTGCGTTCTTCCAGCTTGCGGAAGGTCATTACCT‑CCCCCATATTCACAAACGCAGGTTGATCGGCGTA  >  W3110S.gb/1891263‑1891355                                                            |                                   ttgCAAATAAGCGGCAAACGCGCGACTGCGTTCTTCCAGCTTGCGGAAGGTCATTACCt                                     >  1:1178509/1‑59 (MQ=255) ttgCAAATAAGCGGCAAACGCGCGACTGCGTTCTTCCAGCTTGCGGAAGGTCATTACCt                                     >  1:1455017/1‑59 (MQ=255) ttgCAAATAAGCGGCAAACGCGCGACTGCGTTCTTCCAGCTTGCGGAAGGTCATTACCt                                     >  1:723802/1‑59 (MQ=255) ttgCAAATAAGCGGCAAACGCGCGACTGCGTTCTTCCAGCTTGCGGAAGGTCATTACCt                                     >  1:4339782/1‑59 (MQ=255) ttgCAAATAAGCGGCAAACGCGCGACTGCGTTCTTCCAGCTTGCGGAAGGTCATTACCt                                     >  1:3874256/1‑59 (MQ=255) ttgCAAATAAGCGGCAAACGCGCGACTGCGTTCTTCCAGCTTGCGGAAGGTCATTACCt                                     >  1:2837575/1‑59 (MQ=255) ttgCAAATAAGCGGCAAACGCGCGACTGCGTTCTTCCAGCTTGCGGAAGGTCATTACCT‑cc                                  >  1:956856/1‑61 (MQ=255) ttgCAAATAAGCGGCAAACGCGCGACTGCGTTCTTCCAGCTTGCGGAAGGTCATTACCT‑cc                                  >  1:4466356/1‑61 (MQ=255) ttgCAAATAAGCGGCAAACGCGCGACTGCGTTCTTCCAGCTTGCGGAAGGTCATTACCT‑cc                                  >  1:3814126/1‑61 (MQ=255) ttgCAAATAAGCGGCAAACGCGCGACTGCGTTCTTCCAGCTTGCGGAAGGTCATTACCT‑cc                                  >  1:3741329/1‑61 (MQ=255) ttgCAAATAAGCGGCAAACGCGCGACTGCGTTCTTCCAGCTTGCGGAAGGTCATTACCT‑cc                                  >  1:2505824/1‑61 (MQ=255)                 aaCGCGCGACTGCGTTCTTCCAGCTTGCGGAAGGTCATTACCTCCCCCCATATTCACaaa                    >  1:3027263/1‑60 (MQ=255)                 aaCGCGCGACTGCGTTCTTCCAGCTTGCGGAAGGTCATTACCTCCCCCCATATTCACaaa                    >  1:3314666/1‑60 (MQ=255)                 aaCGCGCGACTGCGTTCTTCCAGCTTGCGGAAGGTCATTACCTCCCCCCATATTCACaaa                    >  1:299351/1‑60 (MQ=255)                 aaCGCGCGACTGCGTTCTTCCAGCTTGCGGAAGGTCATTACCTCCCCCCATATTCACaaa                    >  1:2875597/1‑60 (MQ=255)                 aaCGCGCGACTGCGTTCTTCCAGCTTGCGGAAGGTCATTACCTCCCCCCATATTCACaaa                    >  1:2228616/1‑60 (MQ=255)                 aaCGCGCGACTGCGTTCTTCCAGCTTGCGGAAGGTCATTACCTCCCCCCATATTCACaaa                    >  1:1860826/1‑60 (MQ=255)                                tcttcCAGCTTGCGGAAGGTCATTACCT‑CCCCCATATTCACAAACGCAGGTTGATCGGCGTa  <  1:1122621/62‑1 (MQ=255)                                 cttcCAGCTTGCGGAAGGTCATTACCT‑CCCCCATATTCACAAACGCAGGTTGATCGGCGTa  <  1:1877027/61‑1 (MQ=255)                                                            |                                   TTGCAAATAAGCGGCAAACGCGCGACTGCGTTCTTCCAGCTTGCGGAAGGTCATTACCT‑CCCCCATATTCACAAACGCAGGTTGATCGGCGTA  >  W3110S.gb/1891263‑1891355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑