Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb928,0520AG51.5% 0.2 / 44.0 33R522R (CGT→CGC) cydCfused cysteine transporter subunits and membrane component and ATP‑binding component of ABC superfamily
Reads supporting (aligned to +/- strand):  ref base A (14/2);  new base G (0/17);  total (14/19)
Fisher's exact test for biased strand distribution p-value = 1.47e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TTTGTTGGAAACGAGAGAGTCCGCGAAGTCGATGGGTGACCATTAACACCGTTTTCTCACGCATCATTTCTGCAAGCAATTCAAGGATCTGGCTTTCGGTTGTGGCATCTAAG  >  W3110S.gb/927994‑928106
                                                          |                                                      
tttGTTGGAAACGAGAGAGTCCGCGAAGTCGATGGGTGACCATTAACACCGTTTTCTCACGc                                                     >  1:3776935/1‑62 (MQ=255)
tttGTTGGAAACGAGAGAGTCAGCGAAGTCGATGGGTGACCATTAACACCGTTTTCTCACGc                                                     >  1:3113868/1‑62 (MQ=255)
      ggAAACGAGAGAGTCCGCGAAGTCGATGGGTGACCATTAACACCGTTTTCTCACGcat                                                   >  1:3375431/1‑58 (MQ=255)
             agagagTCCGCGAAGTCGATGGGTGACCATTAACACCGTTTTCTCACGCATCATTTCTgca                                         >  1:2465749/1‑61 (MQ=255)
                 agTCCGCGAAGTCGATGGGTGACCATTAACACCGTTTTCTCACGCATCATTTCTgcaagca                                     >  1:347497/1‑61 (MQ=255)
                 agTCCGCGAAGTCGATGGGTGACCATTAACACCGTTTTCTCACGCATCATTTCTgcaagca                                     >  1:2089600/1‑61 (MQ=255)
                                                ccGTTTTCTCGCACATCATTTCTGCAAGCAATTCAAGGATCTGACTTTCGGTTGTGGCATCt     <  1:2561568/62‑1 (MQ=255)
                                                ccGTTTTCTCGCACATCATTTCTGCAAGCAATTCAAGGATCTGACTTTCGGTTGTGGCATCt     <  1:3771748/62‑1 (MQ=255)
                                                ccGTTTTCTCGCACATCATTTCTGCAAGCAATTCAAGGATCTGACTTTCGGTTGTGGCATCt     <  1:4023361/62‑1 (MQ=255)
                                                ccGTTTTCTCGCACATCATTTCTGCAAGCAATTCAAGGATCTGACTTTCGGTTGTGGCATCt     <  1:4028690/62‑1 (MQ=255)
                                                ccGTTTTCTCGCACATCATTTCTGCAAGCAATTCAAGGATCTGACTTTCGGTTGTGGCATCt     <  1:4067172/62‑1 (MQ=255)
                                                ccGTTTTCTCGCACATCATTTCTGCAAGCAATTCAAGGATCTGACTTTCGGTTGTGGCATCt     <  1:274963/62‑1 (MQ=255)
                                                ccGTTTTCTCGCACATCATTTCTGCAAGCAATTCAAGGATCTGACTTTCGGTTGTGGCATCt     <  1:4315499/62‑1 (MQ=255)
                                                ccGTTTTCTCGCACATCATTTCTGCAAGCAATTCAAGGATCTGACTTTCGGTTGTGGCATCt     <  1:2574020/62‑1 (MQ=255)
                                                ccGTTTTCTCGCACATCATTTCTGCAAGCAATTCAAGGATCTGACTTTCGGTTGTGGCATCt     <  1:2017852/62‑1 (MQ=255)
                                                ccGTTTTCTCGCACATCATTTCTGCAAGCAATTCAAGGATCTGACTTTCGGTTGTGGCATCt     <  1:1900995/62‑1 (MQ=255)
                                                ccGTTTTCTCGCACATCATTTCTGCAAGCAATTCAAGGATCTGACTTTCGGTTGTGGCATCt     <  1:1895615/62‑1 (MQ=255)
                                                ccGTTTTCTCGCACATCATTTCTGCAAGCAATTCAAGGATCTGACTTTCGGTTGTGGCATCt     <  1:1784455/62‑1 (MQ=255)
                                                ccGTTTTCTCGCACATCATTTCTGCAAGCAATTCAAGGATCTGACTTTCGGTTGTGGCATCt     <  1:1740278/62‑1 (MQ=255)
                                                ccGTTTTCTCGCACATCATTTCTGCAAGCAATTCAAGGATCTGACTTTCGGTTGTGGCATCt     <  1:1707403/62‑1 (MQ=255)
                                                ccGTTTTCTCGCACATCATTTCTGCAAGCAATTCAAGGATCTGACTTTCGGTTGTGGCATCt     <  1:495470/62‑1 (MQ=255)
                                                ccGTTTTCTCGCACATCATTTCTGCAAGCAATTCAAGGATCTGACTTTCGGTTGTGGCATCt     <  1:1413110/62‑1 (MQ=255)
                                                ccGTTTTCTCGCACATCATTTCTGCAAGAAATTCAATGATCTGACTTTAGGTTGTGGCATCt     <  1:4287651/62‑1 (MQ=255)
                                                ccGTTTTCTCACGCATCATTTCTGCAAGCAATTCAAGGATCTGGCTTTCGGTTGTGGCAt       >  1:516387/1‑60 (MQ=255)
                                                ccGTTTTCTCACGCATCATTTCTGCAAGCAATTCAAGGATCTGGCTTTCGGTTGTGGCAt       >  1:4385359/1‑60 (MQ=255)
                                                ccGTTTTCTCACGCATCATTTCTGCAAGCAATTCAAGGATCTGGCTTTCGGTTGTGGCAt       >  1:3102960/1‑60 (MQ=255)
                                                ccGTTTTCTCACGCATCATTTCTGCAAGCAATTCAAGGATCTGGCTTTCGGCTGTGGCAt       >  1:356839/1‑60 (MQ=255)
                                                 cGTTTTCTCACGCATCATTTCTGCAAGCAATTCAAGGATCTGGCTTTCGGTtg             <  1:3359392/53‑1 (MQ=255)
                                                 cGTTTTCTCACGCATCATTTCTGCAAGCAATTCAAGGATCTGGCTTTCGGTtg             <  1:2425947/53‑1 (MQ=255)
                                                   ttttCTCACGCATCATTTCTGCAAGCAATTCAAGGATCTGGCTTTCGGTTGTGGCATCTAAg  >  1:3230044/1‑62 (MQ=255)
                                                   ttttCTCACGCATCATTTCTGCAAGCAATTCAAGGATCTGGCTTTCGGTTGTGGCATCTAAg  >  1:2703939/1‑62 (MQ=255)
                                                   ttttCTCACGCATCATTTCTGCAAGCAATTCAAGGATCTGGCTTTCGGTTGTGGCATCTAAg  >  1:155928/1‑62 (MQ=255)
                                                   ttttCTCACGCATCATTTCTGCAAGCAATTCAAGGATCTGGCTTTCGGTTGTGGCATCTAAg  >  1:103573/1‑62 (MQ=255)
                                                          |                                                      
TTTGTTGGAAACGAGAGAGTCCGCGAAGTCGATGGGTGACCATTAACACCGTTTTCTCACGCATCATTTCTGCAAGCAATTCAAGGATCTGGCTTTCGGTTGTGGCATCTAAG  >  W3110S.gb/927994‑928106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑