| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,134,504 | 0 | T | G | 61.3% | 41.6 / 22.5 | 31 | G118G (GGT→GGG) | flgE | flagellar hook protein |
| Reads supporting (aligned to +/- strand): ref base T (5/7); new base G (19/0); total (24/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.01e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATTTAAGCTGGATGAAAACCGTAACCTGGTGAATATGCAAGGTTTACAGCTGACGGGTTACCCGGCAACCGGTACGCCGCCGACTATTCAGCAAGGGGCGAATCCGACCAATATTTCGA > W3110S.gb/1134447‑1134565 | aTTTAAGCTGGATGAAAACCGTAACCTGGTGAATATGCAAGGTTTACCGCTGACGGGGTAcc > 1:3341492/1‑62 (MQ=255)aTTTAAGCTGGATGAAAACCGTAACCTGGTGAATATGCAAGGTTTACAGCTGACGGGGTAcc > 1:2984455/1‑62 (MQ=255)aTTTAAGCTGGATGAAAACCGTAACCTGGTGAATATGCAAGGTTTACAGCTGACGGGGTAcc > 1:890458/1‑62 (MQ=255)aTTTAAGCTGGATGAAAACCGTAACCTGGTGAATATGCAAGGTTTACAGCTGACGGGGTAcc > 1:592924/1‑62 (MQ=255)aTTTAAGCTGGATGAAAACCGTAACCTGGTGAATATGCAAGGTTTACAGCTGACGGGGTAcc > 1:4681930/1‑62 (MQ=255)aTTTAAGCTGGATGAAAACCGTAACCTGGTGAATATGCAAGGTTTACAGCTGACGGGGTAcc > 1:4639836/1‑62 (MQ=255)aTTTAAGCTGGATGAAAACCGTAACCTGGTGAATATGCAAGGTTTACAGCTGACGGGGTAcc > 1:4167890/1‑62 (MQ=255)aTTTAAGCTGGATGAAAACCGTAACCTGGTGAATATGCAAGGTTTACAGCTGACGGGGTAcc > 1:3859104/1‑62 (MQ=255)aTTTAAGCTGGATGAAAACCGTAACCTGGTGAATATGCAAGGTTTACAGCTGACGGGGTAcc > 1:1378754/1‑62 (MQ=255)aTTTAAGCTGGATGAAAACCGTAACCTGGTGAATATGCAAGGTTTACAGCTGACGGGGTAcc > 1:2968102/1‑62 (MQ=255)aTTTAAGCTGGATGAAAACCGTAACCTGGTGAATATGCAAGGTTTACAGCTGACGGGGTAcc > 1:2900526/1‑62 (MQ=255)aTTTAAGCTGGATGAAAACCGTAACCTGGTGAATATGCAAGGTTTACAGCTGACGGGGTAcc > 1:2771834/1‑62 (MQ=255)aTTTAAGCTGGATGAAAACCGTAACCTGGTGAATATGCAAGGTTTACAGCTGACGGGGTAcc > 1:2388238/1‑62 (MQ=255)aTTTAAGCTGGATGAAAACCGTAACCTGGTGAATATGCAAGGTTTACAGCTGACGGGGTAcc > 1:1413881/1‑62 (MQ=255)aTTTAAGCTGGATGAAAACCGTAACCTGGTGAATATGCAAGGTTTACAGCTGACGGGGTAcc > 1:1882534/1‑62 (MQ=255)aTTTAAGCTGGATGAAAACCGTAACCTGGTGAATATGCAAGGTTTACAGCTGACGGGGTAcc > 1:19734/1‑62 (MQ=255)aTTTAAGCTGGATGAAAACCGTAACCTGGTGAATATGCAAGGTTTACAGCTGACGGGGTAcc > 1:208877/1‑62 (MQ=255)aTTTAAGCTGGATGAAAACCGTAACCTGGTGAATATGCAAGGTTTACAGCTGACGGGGTAcc > 1:2356984/1‑62 (MQ=255)aTTTAAGCTGGATGAAAACCGTAACCTGGTGAATATGCAAGGTTTACAGCTGACGGGGTAc > 1:1442090/1‑61 (MQ=255) tAAGCTGGATGAAAACCGTAACCTGGTGAATATGCAAGGTTTACAGCTGACGGGTTACCCgg < 1:3785153/62‑1 (MQ=255) tAAGCTGGATGAAAACCGTAACCTGGTGAATATGCAAGGTTTACAGCTGACGGGTTACCCgg < 1:1921544/62‑1 (MQ=255) gAATATTCAAGGTTTACAGCTGACGGGTTACCCGGCAACCGGTAcgcc < 1:4148782/48‑1 (MQ=255) gAATATGCAAGGTTTACAGCTGACGGGTTACCCGGCAACCGGTAcgcc < 1:1569093/48‑1 (MQ=255) cAAGGTTTACAGCTGACGGGTTACCCGGCAACCGGTACGCCGCCGACt > 1:4020073/1‑48 (MQ=255) tttACAGCTGACGGGTTACCCGGCAACCGGTACGCCGCCGACTATTCAGCAAGGGGCGAATc < 1:3318257/62‑1 (MQ=255) tttACAGCTGACGGGTTACCCGGCAACCGGTACGCCGCCGACTATTCAGCAAGGGGCGAATc < 1:848964/62‑1 (MQ=255) ttACAGCTGACGGGTTACCCGGCAACCGGTACGCCGCCGACTATTCAGCAAGGGGCGAATcc > 1:4145919/1‑62 (MQ=255) ttACAGCTGACGGGTTACCCGGCAACCGGTACGCCGCCGACTATTCAGCAAGGGGCGAATcc > 1:667608/1‑62 (MQ=255) ttACAGCTGACGGGTTACCCGGCAACCGGTACGCCGCCGACTATTCAGCAAGGGGCGAATcc > 1:1593584/1‑62 (MQ=255) ttACAGCTGACGGGTTACCCGGCAACCGGTACGCCGCCGACTATTCAGCAAGGGGCGAATcc > 1:4106824/1‑62 (MQ=255) ttACAGCTGACGGGTTACCCGGCAACCGGTACGCCGCCGACTATTCAGCAAGGGGCGAATc < 1:2257314/61‑1 (MQ=255) ttACCCGGCAACCGGTACGCCGCCGACTATTCAGCAAGGGGCGAATc < 1:2436726/47‑1 (MQ=255) ttACCCGGCAACCGGTACGCCGCCGACTATTCAGCAAGGGGCGAATCCGACCAATATTTCGa < 1:2840775/62‑1 (MQ=255) ttACCCGGCAACCGGTACGCCGCCGACTATTCAGCAAGGGGCGAATCCGACCAATATTTCGa < 1:406812/62‑1 (MQ=255) | ATTTAAGCTGGATGAAAACCGTAACCTGGTGAATATGCAAGGTTTACAGCTGACGGGTTACCCGGCAACCGGTACGCCGCCGACTATTCAGCAAGGGGCGAATCCGACCAATATTTCGA > W3110S.gb/1134447‑1134565 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |