Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,137,2360GA63.3% 21.1 / 28.5 30Q32Q (CAG→CAAflgHflagellar protein of basal‑body outer‑membrane L ring
Reads supporting (aligned to +/- strand):  ref base G (11/0);  new base A (0/19);  total (11/19)
Fisher's exact test for biased strand distribution p-value = 1.83e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01
Rejected as consensus: Frequency below/above cutoff threshold.

AGCTTGTTGGTGCTTTCACTAACCGGCTGCGCCTGGATACCCTCCACGCCGCTGGTGCAGGGGGCGACCAGTGCACAACCGGTTCCCGGTCCGACGCCCGTCGCCAACGGTTC  >  W3110S.gb/1137177‑1137289
                                                           |                                                     
aGCTTGTTGGTGCTTTCACTAACCGGCTGCGCCTGGATACCCTCCACGCCGCTGGTGCAggg                                                     >  1:1491820/1‑62 (MQ=255)
aGCTTGTTGGTGCTTTCACTAACCGGCTGCGCCTGGATACCCTCCACGCCGCTGGTGCAggg                                                     >  1:3567201/1‑62 (MQ=255)
    tgttgGTGCTTTCACTAACCGGCTGCGCCTGGATACCCTCCACGCCGCTGGTGCAGGGGGCg                                                 >  1:2073123/1‑62 (MQ=255)
    tgttgGTGCTTTCACTAACCGGCTGCGCCTGGATACCCTCCACGCCGCTGGTGCAGGGGGCg                                                 >  1:4401290/1‑62 (MQ=255)
    tgttgGTGCTTTCACTAACCGGCTGCGCCTGGATACCCTCCACGCCGCTGGTGCAGGGGGCg                                                 >  1:1032082/1‑62 (MQ=255)
                               ccTGTATACCCTCCACGCCGCTGGTACAAGGGGCGACCAGTGCACAACCGGTTCCCGGTCCg                      <  1:1334822/62‑1 (MQ=255)
                               ccTGGATACCCTCCACGCCGCTGGTGCAGGGGGCGACCAg                                            >  1:1347344/1‑40 (MQ=255)
                               ccTGGATACCCTCCACGCCGCTGGTGCAGGGGGCGACCAGTGCACAACCGGTTCCCGGTCCg                      >  1:2171459/1‑62 (MQ=255)
                               ccTGGATACCCTCCACGCCGCTGGTACAAGGGGCGACCAGTGCACAACCGGTTCCCGGTCCg                      <  1:1116174/62‑1 (MQ=255)
                               ccTGGATACCCTCCACGCCGCTGGTACAAGGGGCGACCAGTGCACAACCGGTTCCCGGTCCg                      <  1:735837/62‑1 (MQ=255)
                               ccTGGATACCCTCCACGCCGCTGGTACAAGGGGCGACCAGTGCACAACCGGTTCCCGGTCCg                      <  1:655969/62‑1 (MQ=255)
                               ccTGGATACCCTCCACGCCGCTGGTACAAGGGGCGACCAGTGCACAACCGGTTCCCGGTCCg                      <  1:851932/62‑1 (MQ=255)
                               ccTGGATACCCTCCACGCCGCTGGTACAAGGGGCGACCAGTGCACAACCGGTTCCCGGTCCg                      <  1:4593295/62‑1 (MQ=255)
                               ccTGGATACCCTCCACGCCGCTGGTACAAGGGGCGACCAGTGCACAACCGGTTCCCGGTCCg                      <  1:4262100/62‑1 (MQ=255)
                               ccTGGATACCCTCCACGCCGCTGGTACAAGGGGCGACCAGTGCACAACCGGTTCCCGGTCCg                      <  1:3634147/62‑1 (MQ=255)
                               ccTGGATACCCTCCACGCCGCTGGTACAAGGGGCGACCAGTGCACAACCGGTTCCCGGTCCg                      <  1:905107/62‑1 (MQ=255)
                               ccTGGATACCCTCCACGCCGCTGGTACAAGGGGCGACCAGTGCACAACCGGTTCCCGGTCCg                      <  1:2887964/62‑1 (MQ=255)
                               ccTGGATACCCTCCACGCCGCTGGTACAAGGGGCGACCAGTGCACAACCGGTTCCCGGTCCg                      <  1:2833776/62‑1 (MQ=255)
                               ccTGGATACCCTCCACGCCGCTGGTACAAGGGGCGACCAGTGCACAACCGGTTCCCGGTCCg                      <  1:2499649/62‑1 (MQ=255)
                               ccTGGATACCCTCCACGCCGCTGGTACAAGGGGCGACCAGTGCACAACCGGTTCCCGGTCCg                      <  1:1487107/62‑1 (MQ=255)
                                cTGGATACCCTCCACGCCGCTGGTACAAGGGGCGACCAGTGCACAACCGGTTCCCGGTCCg                      <  1:2841728/61‑1 (MQ=255)
                                    aTACCCTCCACGCCGCTGGTACAAGGGGCGACCAGTGCACAACCGGTTCCCGGTCCg                      <  1:2125279/57‑1 (MQ=255)
                                       cccTCCACGCCGCTGGTACAAGGGGCGACCAGTGCACAACCGGTTCCCGGTCCg                      <  1:3864881/54‑1 (MQ=255)
                                       cccTCCACGCCGCTGGTACAAGGGGCGACCAGTGCACAACCGGTTCCCGGTCCg                      <  1:676832/54‑1 (MQ=255)
                                        ccTCCACGCCGCTGGTACAAGGGGCGACCAGTGCACAACCGGTTCCCGGTCCg                      <  1:846159/53‑1 (MQ=255)
                                         cTCCACGCCGCTGGTGCAGGGGGCGACCAGTGCACAACCGGTTCCCGGTCCGACGCCCGTc             >  1:2943360/1‑61 (MQ=255)
                                         cTCCACGCCGCTGGTGCAGGGGGCGACCAGTGCACAACCGGTTCCCGGTCCGACGCCCGTc             >  1:4229599/1‑61 (MQ=255)
                                         cTCCACGCCGCTGGTGCAGGGGGCGACCAGTGCACAACCGGTTCCCGGTCCGACGCCCGTc             >  1:2766203/1‑61 (MQ=255)
                                         cTCCACGCCGCTGGTACAAGGGGCGACCAGTGCACAACCGGTTCCCGGTCCg                      <  1:962390/52‑1 (MQ=255)
                                                   cTGGTGCAGGGGGCGACCAGTGCACAACCGGTTCCCGGTCCGACGCCCGTCGCCAACGGTTc  >  1:626185/1‑62 (MQ=255)
                                                   cTGGTGCAGGGGGCGACCAGTGCACAACCGGTTCCCGGTCCGACGCCCGTCGCCAACGGTTc  >  1:3060057/1‑62 (MQ=255)
                                                   cTGGTGCAGGGGGCGACCAGTGCACAACCGGTTCCCGGTCCGACGCCCGTCGCCAACGGTTc  >  1:2778571/1‑62 (MQ=255)
                                                           |                                                     
AGCTTGTTGGTGCTTTCACTAACCGGCTGCGCCTGGATACCCTCCACGCCGCTGGTGCAGGGGGCGACCAGTGCACAACCGGTTCCCGGTCCGACGCCCGTCGCCAACGGTTC  >  W3110S.gb/1137177‑1137289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: