| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,263,637 | 0 | G | A | 55.6% | 3.9 / 31.9 | 27 | A273V (GCT→GTT) | ispE | 4‑diphosphocytidyl‑2‑C‑methylerythritol kinase |
| Reads supporting (aligned to +/- strand): ref base G (1/11); new base A (0/15); total (1/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GAAACTCAGCTTGCCCGGCTTAAAGCATGGCTCTGTGCAATGGGGAAAGATTAGCGCCTTTCGCCACAAAGCCATTGAGCCATTCCGGGGCTTGCTCT > W3110S.gb/1263584‑1263681 | gAAACTCAGCTTGCCCGGCTTAAAGCATGGCTCTGTGCAATGGGGAAAGATTAACGCCTTTc < 1:1241733/62‑1 (MQ=255)gAAACTCAGCTTGCCCGGCTTAAAGCATGGCTCTGTGCAATGGGGAAAGATTAACGCCTTTc < 1:897990/62‑1 (MQ=255)gAAACTCAGCTTGCCCGGCTTAAAGCATGGCTCTGTGCAATGGGGAAAGATTAACGCCTTTc < 1:529234/62‑1 (MQ=255)gAAACTCAGCTTGCCCGGCTTAAAGCATGGCTCTGTGCAATGGGGAAAGATTAACGCCTTTc < 1:4640286/62‑1 (MQ=255)gAAACTCAGCTTGCCCGGCTTAAAGCATGGCTCTGTGCAATGGGGAAAGATTAACGCCTTTc < 1:3672142/62‑1 (MQ=255)gAAACTCAGCTTGCCCGGCTTAAAGCATGGCTCTGTGCAATGGGGAAAGATTAACGCCTTTc < 1:3619632/62‑1 (MQ=255)gAAACTCAGCTTGCCCGGCTTAAAGCATGGCTCTGTGCAATGGGGAAAGATTAACGCCTTTc < 1:3279335/62‑1 (MQ=255)gAAACTCAGCTTGCCCGGCTTAAAGCATGGCTCTGTGCAATGGGGAAAGATTAACGCCTTTc < 1:2484940/62‑1 (MQ=255)gAAACTCAGCTTGCCCGGCTTAAAGCATGGCTCTGTGCAATGGGGAAAGATTAACGCCTTTc < 1:217610/62‑1 (MQ=255)gAAACTCAGCTTGCCCGGCTTAAAGCATGGCTCTGTGCAATGGGGAAAGATTAACGCCTTTc < 1:131284/62‑1 (MQ=255)gAAACTCAGCTTGCCCGGCTTAAAGCATGGCTCTGTGCAATGGGGAAAGATTAACGCCTTTc < 1:1507792/62‑1 (MQ=255)gAAACTCAGCTTGCCCGGCTTAAAGCATGGCTCTGTGCAATGGGGAAAGATTAACGCCTTTc < 1:1916286/62‑1 (MQ=255)gAAACTCAGCTTGCCCGGCTTAAAGCATGGCTCTGTGCAATGGGGAAAGATTAACGCCTTTc < 1:152797/62‑1 (MQ=255)gAAACTCAGCTTGCCCGGCTTAAAGCATGGCTCTGTGCAATGGGGAAAGATTAACGCCTTTc < 1:1520437/62‑1 (MQ=255) cTCAGCTTGCCCGGCTTAAAGCATGGCTCTGTGCAATGGGGAAAGATTAGCGCCTTTCGCca < 1:2436763/62‑1 (MQ=255) cTCAGCTTGCCCGGCTTAAAGCATGGCTCTGTGCAATGGGGAAAGATTAGCGCCTTTCGCca < 1:3112131/62‑1 (MQ=255) cTCAGCTTGCCCGGCTTAAAGCATGGCTCTGTGCAATGGGGAAAGATTAGCGCCTTTCGCca < 1:4610079/62‑1 (MQ=255) cTCAGCTTGCCCGGCTTAAAGCATGGCTCTGTGCAATGGGGAAAGATTAGCGCCTTTCGCca < 1:2308467/62‑1 (MQ=255) cTCAGCTTGCCCGGCTTAAAGCATGGCTCTGTGCAATGGGGAAAGATTAGCGCCTTTCGCca < 1:369100/62‑1 (MQ=255) tCAGCTTGCCCGGCTTAAAGCATGGCTCTGTGCAATGGGGAAAGATTAACGCCTTTc < 1:3648070/57‑1 (MQ=255) cTTGCCCGGCTTATAGCATGGCTCTGTGCAATGGGGAAAGATTAGCGCCTTTCGCCACAAAg < 1:3437850/62‑1 (MQ=255) cTTGCCCGGCTTAAAGCATGGCTCTGTGCAATGGGGAAAGATTAGCGCCTTTCGCCACAAAg < 1:594044/62‑1 (MQ=255) tGCCCGGCTTAAAGCATGGCTCTGTGCAATGGGGAAAGATTAGCGCCTTTCGCCACAAAGcc < 1:4190597/62‑1 (MQ=255) tGCCCGGCTTAAAGCATGGCTCTGTGCAATGGGGAAAGATTAGCGCCTTTCGCCACAAAGcc < 1:1101386/62‑1 (MQ=255) gCTCTGTGCAATGGGGAAAGATTAGCGCCTTTCGCCACAAAGCCATTGAGCCATTCCGGGGc < 1:302478/62‑1 (MQ=255) gCTCTGTGCAATGGGGAAAGATTAGCGCCTTTCGCCACAAAGCCATTGAGCCATTCCGGGGc < 1:726576/62‑1 (MQ=255) gCAATGGGGAAAGATTAGCGCCTTTCGCCACAAAGCCATTGAGCCATTCCGGGGCTTGttct > 1:3264518/1‑62 (MQ=255) | GAAACTCAGCTTGCCCGGCTTAAAGCATGGCTCTGTGCAATGGGGAAAGATTAGCGCCTTTCGCCACAAAGCCATTGAGCCATTCCGGGGCTTGCTCT > W3110S.gb/1263584‑1263681 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |