| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,622,293 | 0 | T | A | 51.7% | ‑5.7 / 42.0 | 29 | I187F (ATT→TTT) | eamA | cysteine and O‑acetyl‑L‑serine efflux system |
| Reads supporting (aligned to +/- strand): ref base T (7/7); new base A (15/0); total (22/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.20e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.73e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGA > W3110S.gb/1622239‑1622353 | tgtgAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAAAGag > 1:18948/1‑57 (MQ=255)tgtgAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAAAGag > 1:3782042/1‑57 (MQ=255)tgtgAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAAAGag > 1:1103348/1‑57 (MQ=255)tgtgAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAAAGag > 1:4510999/1‑57 (MQ=255)tgtgAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAAAGag > 1:2633532/1‑57 (MQ=255)tgtgAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAAAGag > 1:585457/1‑57 (MQ=255)tgtgAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAAAGag > 1:588851/1‑57 (MQ=255)tgtgAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAAAGag > 1:642584/1‑57 (MQ=255)tgtgAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAAAGag > 1:1894653/1‑57 (MQ=255)tgtgAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAAAGag > 1:649939/1‑57 (MQ=255)tgtgAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAAAGag > 1:1607551/1‑57 (MQ=255)tgtgAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAAAGag > 1:1499838/1‑57 (MQ=255)tgtgAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAAAGag > 1:705374/1‑57 (MQ=255)tgtgAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAAAGag > 1:1222422/1‑57 (MQ=255)tgtgAATCATGGTTGCGGAACCATAGAGAATCAGCGAGGCAACAAAGAAGGGAAAGag > 1:1704998/1‑57 (MQ=255) tGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAgcg > 1:3452074/1‑62 (MQ=255) tGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAgcg > 1:3905533/1‑62 (MQ=255) tGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAgcg > 1:211913/1‑62 (MQ=255) tGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAgcg > 1:1640128/1‑62 (MQ=255) cGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGAt > 1:2692796/1‑49 (MQ=255) cGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAg > 1:4265186/1‑62 (MQ=255) aCCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTa < 1:526886/62‑1 (MQ=255) aCCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTa < 1:245523/62‑1 (MQ=255) aCCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTa < 1:92827/62‑1 (MQ=255) aTCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCa < 1:3569824/62‑1 (MQ=255) aTCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCa < 1:4320890/62‑1 (MQ=255) aTCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCa < 1:1316831/62‑1 (MQ=255) aGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCAc > 1:948874/1‑62 (MQ=255) aTGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGa < 1:2416915/62‑1 (MQ=255) | TGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGA > W3110S.gb/1622239‑1622353 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |