| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,058,259 | 0 | C | T | 52.6% | ‑1.8 / 53.3 | 38 | A354A (GCC→GCT) | amn | AMP nucleosidase |
| Reads supporting (aligned to +/- strand): ref base C (13/5); new base T (20/0); total (33/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.71e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGCCGCCCGATATTCCTATTCCGAGCATTGCTGAAGTGCAACGTGCGCTTTATGACGCCACCAAGCTGGTGAGCGGCAGGCCCGGTGAGGAAGTCAAACAGCGGCTACGTACTGGTAC > W3110S.gb/2058201‑2058318 | tGCCGCCCGATATTCCTATTCCGAGCATTGCTGAAGTGCAACGTGCGCTTTATGACGcca > 1:1275122/1‑60 (MQ=255)tGCCGCCCGATATTCCTATTCCGAGCATTGCTGAAGTGCAACGTGCGCTTTATGACGcca > 1:1296939/1‑60 (MQ=255)tGCCGCCCGATATTCCTATTCCGAGCATTGCTGAAGTGCAACGTGCGCTTTATGACGcca > 1:3411156/1‑60 (MQ=255)tGCCGCCCGATATTCCTATTCCGAGCATTGCTGAAGTGCAACGTGCGCTTTATGACGcca > 1:1854241/1‑60 (MQ=255)tGCCGCCCGATATTCCTATTCCGAGCATTGCTGAAGTGCAACGTGCGCTTTATGACGaca > 1:1891308/1‑60 (MQ=255)tGCCGCCCGATATTCCTATTCCGAGAATTGCTGAAGTGCAACGTGCGCTTTATGACGcca > 1:1114331/1‑60 (MQ=255) cGATATTCCTATTCCGAGCATTGCTGAAGTGCAACGTGCGCTTTATGACGCCACCAAGCTgg < 1:2808473/62‑1 (MQ=255) cGATATTCCTATTCCGAGCATTGCTGAAGTGCAACGTGCGCTTTATGACGCCACCAAGCTgg < 1:3538143/62‑1 (MQ=255) cctattccGAGCATTGCTTAAGTGCAACGTGCGCTTTATGACGCTACCAAGTTAGTCAGc > 1:2836241/1‑60 (MQ=255) cctattccGAGCATTGCTGAAGTGCAACGTGCGCTTTATGACGCTACCAAGTTGGTCAGc > 1:1028719/1‑60 (MQ=255) cctattccGAGCATTGCTGAAGTGCAACGTGCGCTTTATGACGCTACCAAGTTAGTCAGc > 1:2500207/1‑60 (MQ=255) cctattccGAGCATTGCTGAAGTGCAACGTGCGCTTTATGACGCTACCAAGTTAGTCAGc > 1:92479/1‑60 (MQ=255) cctattccGAGCATTGCTGAAGTGCAACGTGCGCTTTATGACGCTACCAAGTTAGTCAGc > 1:4705588/1‑60 (MQ=255) cctattccGAGCATTGCTGAAGTGCAACGTGCGCTTTATGACGCTACCAAGTTAGTCAGc > 1:3149456/1‑60 (MQ=255) cctattccGAGCATTGCTGAAGTGCAACGTGCGCTTTATGACGCTACCAAGTTAGTCAGc > 1:3665349/1‑60 (MQ=255) cctattccGAGCATTGCTGAAGTGCAACGTGCGCTTTATGACGCTACCAAGTTAGTCAGc > 1:3849245/1‑60 (MQ=255) cctattccGAGCATTGCTGAAGTGCAACGTGCGCTTTATGACGCTACCAAGTTAGTCAGc > 1:4112169/1‑60 (MQ=255) cctattccGAGCATTGCTGAAGTGCAACGTGCGCTTTATGACGCTACCAAGTTAGTCAGc > 1:4241685/1‑60 (MQ=255) cctattccGAGCATTGCTGAAGTGCAACGTGCGCTTTATGACGCTACCAAGTTAGTCAGc > 1:4268956/1‑60 (MQ=255) cctattccGAGCATTGCTGAAGTGCAACGTGCGCTTTATGACGCTACCAAGTTAGTCAGc > 1:4597108/1‑60 (MQ=255) cctattccGAGCATTGCTGAAGTGCAACGTGCGCTTTATGACGCTACCAAGTTAGTCAGc > 1:482445/1‑60 (MQ=255) cctattccGAGCATTGCTGAAGTGCAACGTGCGCTTTATGACGCTACCAAGTTAGTCAGc > 1:600513/1‑60 (MQ=255) cctattccGAGCATTGCTGAAGTGCAACGTGCGCTTTATGACGCTACCAAGTTAGTCAGc > 1:1620757/1‑60 (MQ=255) cctattccGAGCATTGCTGAAGTGCAACGTGCGCTTTATGACGCTACCAAGTTAGTCAGc > 1:1786523/1‑60 (MQ=255) cctattccGAGCATTGCTGAAGTGCAACGTGCGCTTTATGACGCTACCAAGTTAGTCAGc > 1:184385/1‑60 (MQ=255) cctattccGAGCATTGCTGAAGTGCAACGTGCGCTTTATGACGCTACCAAGTTAGTCAGc > 1:2128896/1‑60 (MQ=255) cctattccGAGCATTGCTGAAGTGCAACGTGCGCTTTATGACGCTACCAAGTTAGTCAGc > 1:2166440/1‑60 (MQ=255) cctattccGAGCATTGCTGAAGTGCAACGTGCGCTTTATGACGCTACCAAGTTAGTCAGc > 1:2315917/1‑60 (MQ=255) ttGCTGAAGTGCAACGTGCGCTTTATGACGCCACCAAGCTgg < 1:223021/42‑1 (MQ=255) aaGTGCAACGTGCGCTTTATGACGCCACCAAGCTgg < 1:1656684/36‑1 (MQ=255) tGCAACGTGCGCTTTATGACGCCACCAAGCTGGTGAGCGGCAGGCCCGGTGAGGAAGTCa > 1:3339997/1‑60 (MQ=255) tGCAACGTGCGCTTTATGACGCCACCAAGCTGGTGAGCGGCAGGCCCGGTGAGGAAGTCa > 1:4181713/1‑60 (MQ=255) gTGCGCTTTATGACGCCACCAAGCTGGTGAGCGGCAGGCCCGGTGAGGAAGTCAAACAGCg > 1:2965724/1‑61 (MQ=255) gTGCGCTTTATGACGCCACCAAGCTGGTGAGCGGCAGGCCCGGTGAGGAAGTCAAACAGCg > 1:1322403/1‑61 (MQ=255) gTGCGCTTTATGACGCCACCAAGCTGGTGAGCGGCAGGCCCGGTGAGGAAGTCAAACAGCg > 1:1316993/1‑61 (MQ=255) cgcTTTATGACGCCACCAAGCTGGTGAGCGGCAGGCCCGGTGAGGAAGTCAAACAGCGGCt > 1:2288354/1‑61 (MQ=255) cgcTTTATGACGCCACCAAGCTGGTGAGCGGCAGGCCCGGTGAGGAAGTCAAACAGCGGCTa > 1:4445106/1‑62 (MQ=255) tATGACGCCACCGAGCTGGTGAGCGGCAGGCCCGGTGAGGAAGTCAAACAGCGGCTACgtac > 1:2428217/1‑62 (MQ=255) tATGACGCCACCAAGCTGGTGAGCGGCAGGCCCGGTGAGGAAGTCAAACAGCGGCTACgtac > 1:2652133/1‑62 (MQ=255) tATGACGCCACCAAGCTGGTGAGCGGCAGGCCCGGTGAGGAAGTCAAACAGCGGCTACgtac > 1:1501229/1‑62 (MQ=255) tGACGCCACCAAGCTGGTGAGCGGCAGGCCCGGTGAGGAAGTCAAACAGCGGCTACgtact > 1:4569038/1‑61 (MQ=255) tGACGCCACCAAGCTGGTGAGCGGCAGGCCCGGTGAGGAAGTCAAACAGCGGCTACgtact > 1:2798871/1‑61 (MQ=255) tGACGCCACCAAGCTGGTGAGCGGCAGGCCCGGTGAGGAAGTCAAACAGCGGCTACgtact > 1:1063671/1‑61 (MQ=255) gCCACCAAGCTGGTGAGCGGCAGGCCCGGTGAGGAAGTCAAACAGCGGCTACgtactggtac < 1:2468978/62‑1 (MQ=255) ccaccaAGCTGGTGAGCGGCAGGCCCGGTGAGGAAGTCAAACAGCGGCTACgtactggtac < 1:923900/61‑1 (MQ=255) | TGCCGCCCGATATTCCTATTCCGAGCATTGCTGAAGTGCAACGTGCGCTTTATGACGCCACCAAGCTGGTGAGCGGCAGGCCCGGTGAGGAAGTCAAACAGCGGCTACGTACTGGTAC > W3110S.gb/2058201‑2058318 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |