Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,952,845	0	C	T	56.2%	0.4 / 16.5	16	T605T (ACG→ACA)	recB	exonuclease V (RecBCD complex), beta subunit
Reads supporting (aligned to +/- strand):  ref base C (4/3);  new base T (9/0);  total (13/3)
Fisher's exact test for biased strand distribution p-value = 6.25e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TTGCCAGCGCACTACGCAGGGTGTTCTCACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTC  >  W3110S.gb/2952808‑2952895                                      |                                                   ttGCCAGCGCACTACGCAGGGTGTTCTCACGTTCGGGCg                                                   >  1:3435280/1‑39 (MQ=255) ttGCCAGCGCACTACGCAGGGTGTTCTCACGTTCGGGCGTCATCACCGCCTGCAACAACCaa                            >  1:2872141/1‑62 (MQ=255)                   gggTGTTCTCACGTTCGGGTGTCATCACCGCCTGCAACAGCCAAAGCATCTCCTgt                >  1:3889184/1‑55 (MQ=255)                   gggTGTTCTCACGTTCGGGTGTCATCACCGCCTGCAACAGCCAAAGCATCTCCTGTGCt             >  1:1041945/1‑59 (MQ=255)                   gggTGTTCTCACGTTCGGGTGTCATCACCGCCTGCAACAGCCAAAGCATCTCCTGTGCt             >  1:1068359/1‑59 (MQ=255)                   gggTGTTCTCACGTTCGGGTGTCATCACCGCCTGCAACAGCCAAAGCATCTCCTGTGCt             >  1:196660/1‑59 (MQ=255)                   gggTGTTCTCACGTTCGGGTGTCATCACCGCCTGCAACAGCCAAAGCATCTCCTGTGCt             >  1:2464463/1‑59 (MQ=255)                   gggTGTTCTCACGTTCGGGTGTCATCACCGCCTGCAACAGCCAAAGCATCTCCTGTGCt             >  1:3011161/1‑59 (MQ=255)                   gggTGTTCTCACGTTCGGGTGTCATCACCGCCTGCAACAGCCAAAGCATCTCCTGTGCt             >  1:323707/1‑59 (MQ=255)                   gggTGTTCTCACGTTCGGGTGTCATCACCGCCTGCAACAGCCAAAGCATCTCCTGTGCt             >  1:3988788/1‑59 (MQ=255)                   gggTGTTCTCACGTTCGGGTGTCATCACCGCCTGCAACAGCCAAAGCATCTCCTGTGCt             >  1:4042799/1‑59 (MQ=255)                          ctcACGTTCGGGCGTCATCACCGCCTGCAACAACCa                             <  1:2017256/36‑1 (MQ=255)                          ctcACGTTCGGGCGTCATCACCGCCTGCAACAACCa                             <  1:3565524/36‑1 (MQ=255)                          ctcACGTTCGGGCGTCATCACCGCCTGCAACAACCa                             <  1:4354573/36‑1 (MQ=255)                            cACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTc  >  1:2101259/1‑61 (MQ=255)                                 tCGGGCGTCATCACCGCCTGCAACAACCAAAGCAtt                      >  1:4487763/1‑36 (MQ=255)                                      |                                                   TTGCCAGCGCACTACGCAGGGTGTTCTCACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTC  >  W3110S.gb/2952808‑2952895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑