Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	47,858	G→A	S30S (TCG→TCA)	kefC →	potassium:proton antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	47,858	0	G	A	100.0%	144.9 / NA	43	S30S (TCG→TCA)	kefC	potassium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/39);  total (4/39)

TTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGG  >  W3110S.gb/47801‑47903                                                          |                                              ttATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCAGt                                             <  1:2266138/60‑1 (MQ=255)      tcGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCAGTACTTgg                                       <  1:866510/61‑1 (MQ=255)      tcGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCAGTACTTgg                                       <  1:760830/61‑1 (MQ=255)      tcGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCAGTACTTgg                                       <  1:3472461/61‑1 (MQ=255)      tcGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCAGTACTTgg                                       <  1:438507/61‑1 (MQ=255)      tcGGTTCGGCAGCGCTGATTGTAACCATTGCGGTACGTCTTGGTCTGGGATCAGTACTTgg                                       <  1:2054525/61‑1 (MQ=255)        ggTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCAGTACTTGGCTa                                    >  1:874168/1‑62 (MQ=255)        ggTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCAGTACTTGGCTa                                    >  1:281420/1‑62 (MQ=255)                aGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGTGATCAGTACTTGGCTACCTGATCg                            <  1:3261351/62‑1 (MQ=255)                aGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCAGTACTTGGCTACCTGATCg                            <  1:2441461/62‑1 (MQ=255)                aGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCAGTACTTGGCTACCTGATCg                            <  1:382044/62‑1 (MQ=255)                aGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCAGTACTTGGCTACCTGATCg                            <  1:3678131/62‑1 (MQ=255)                aGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCAGTACTTGGCTACCTGATCg                            <  1:476788/62‑1 (MQ=255)                 gcgcTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCAGTACTTGGCTACCTGATCg                            <  1:4667248/61‑1 (MQ=255)                  cgcTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCAGTACTTGGCTACCTGATCGcc                          <  1:2634725/62‑1 (MQ=255)                   gcTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCAGTACTTGGCTACCTGATCGCCg                         <  1:2004389/62‑1 (MQ=255)                   gcTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCAGTACTTGGCTACCTGATCGCCg                         <  1:4193037/62‑1 (MQ=255)                   gcTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCAGTACTTGGCTACCTGATCGCCg                         <  1:4192433/62‑1 (MQ=255)                   gcTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCAGTACTTGGCTACCTGATCGCCg                         <  1:4557087/62‑1 (MQ=255)                   gcTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCAGTACTTGGCTACCTGATCGCCg                         <  1:4055416/62‑1 (MQ=255)                   gcTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCAGTACTTGGCTACCTGATCGCCg                         <  1:2639544/62‑1 (MQ=255)                   gcTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCAGTACTTGGCTACCTGATCGCCg                         <  1:2645140/62‑1 (MQ=255)                    cTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCAGTACTTGGCTACCTGATCGCCgg                        <  1:4293932/62‑1 (MQ=255)                    cTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCAGTACTTGGCTACCTGATCGCCgg                        <  1:1028438/62‑1 (MQ=255)                    cTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCAGTACTTGGCTACCTGATCGCCgg                        <  1:2127946/62‑1 (MQ=255)                          gTACCCATTGCGGTACGTCTTGGTCTGGGATCAGTTCTTGGCTACCTGATCGc                           <  1:1646192/53‑1 (MQ=38)                          gTACCCATTGCGGTACGTCTTGGTCTGGGATCAGTACTTGGCTACCTGATCGc                           <  1:1026721/53‑1 (MQ=255)                          gTACCCATTGCGGTACGTCTTGGTCTGGGATCAGTACTTGGCTACCTGATCGc                           <  1:3081762/53‑1 (MQ=255)                          gTACCCATTGCGGTACGTCTTGGTCTGGGATCAGTACTTGGCTACCTGATCGc                           <  1:1475013/53‑1 (MQ=255)                          gTACCCATTGCGGTACGTCTTGGTCTGGGATCAGTACTTGGCTACCTGATCGc                           <  1:771767/53‑1 (MQ=255)                          gTACCCATTGCGGTACGTCTTGGTCTGGGATCAGTACTTGGCTACCTGATCGc                           <  1:1635937/53‑1 (MQ=255)                          gTACCCATTGCGGTACGTCTTGGTCTGGGATCAGTACTTGGCTACCTGATCGc                           <  1:1844160/53‑1 (MQ=255)                          gTACCCATTGCGGTACGTCTTGGTCTGGGATCAGTACTTGGCTACCTGATCGc                           <  1:2062604/53‑1 (MQ=255)                          gTACCCATTGCGGTACGTCTTGGTCTGGGATCAGTACTTGGCTACCTGATCGc                           <  1:2637473/53‑1 (MQ=255)                          gTACCCATTGCGGTACGTCTTGGTCTGGGATCAGTACTTGGCTACCTGATCGc                           <  1:3760766/53‑1 (MQ=255)                          gTACCCATTGCGGTACGTCTTGGTCTGGGATCAGTACTTGGCTACCTGATCGc                           <  1:3076373/53‑1 (MQ=255)                            aCCCATTGCGGTACGTCTTGGTCTGGGATCAGTACTTGGCTACCTGATCGCCGGCTGCatt                 <  1:2204993/61‑1 (MQ=255)                            aCCCATTGCGGTACGTCTTGGTCTGGGATCAGTACTTGGCTACCTGATCGCCGGCTGCatt                 <  1:1486315/61‑1 (MQ=255)                            aCCCATTGCGGTACGTCTTGGTCTGGGATCAGTACTTGGCTACCTGATCGCCGGCTGCatt                 <  1:3532305/61‑1 (MQ=255)                                   gCGGTACGTCTTGGTCTGGGATCAGTACTTGGCTACCTGATCGCCgg                        <  1:2547085/47‑1 (MQ=255)                                        aCGTCTTGGTCTGGGATCAGTACTTGGCTACCTGATCg                            <  1:3846743/38‑1 (MQ=255)                                          gTCTTGGTCTGGGATCAGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTggggg  >  1:2176056/1‑62 (MQ=255)                                          gTCTTGGTCTGGGATCAGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTggggg  >  1:4587184/1‑62 (MQ=255)                                                          |                                              TTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGG  >  W3110S.gb/47801‑47903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑