Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	349,140	C→T	intergenic (+344/‑96)	prpB → / → prpC	2‑methylisocitrate lyase/2‑methylcitrate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	349,140	0	C	T	100.0%	22.6 / NA	10	intergenic (+344/‑96)	prpB/prpC	2‑methylisocitrate lyase/2‑methylcitrate synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/5);  total (5/5)

CGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATC  >  W3110S.gb/349091‑349189                                                  |                                                  cGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCTTACGGGGtgaac                                       <  1:1331741/62‑1 (MQ=25) cGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCTTACGGGGtgaac                                       <  1:1355010/62‑1 (MQ=25) cGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCTTACGGGGtgaac                                       <  1:2982744/62‑1 (MQ=25) cGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCTTACGGGGtgaac                                       <  1:566872/62‑1 (MQ=25)    aTCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCTTACGGGGtgaactga                                    >  1:3889011/1‑62 (MQ=25)    aTCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCTTACGGGGtgaactga                                    >  1:3981280/1‑62 (MQ=25)    aTCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCTTACGGGGtgaactga                                    >  1:58540/1‑62 (MQ=25)                                 gACGCGACTTATCAGGCTTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGt        >  1:1943514/1‑61 (MQ=25)                                 gACGCGACTTATCAGGCTTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGt        >  1:4629673/1‑61 (MQ=25)                                      gACTTATCAGGCTTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATc  <  1:180982/62‑1 (MQ=25)                                                  |                                                  CGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATC  >  W3110S.gb/349091‑349189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑