Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,220,824	C→A	P235T (CCG→ACG)	ebgR →	DNA‑binding transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,220,824	0	C	A	100.0%	68.7 / NA	22	P235T (CCG→ACG)	ebgR	DNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (6/16);  total (6/16)

TTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATT  >  W3110S.gb/3220768‑3220851                                                         |                            tttCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATACGAAg                        <  1:3209315/62‑1 (MQ=255)       gTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATACGAAGGCACtg                  >  1:3779547/1‑62 (MQ=255)       gTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATACGAAGGCACtg                  >  1:302003/1‑62 (MQ=255)             cgGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATACGAAGGCACtgtttgt             <  1:4178200/61‑1 (MQ=255)             cgGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATACGAAGGCACtgtttgt             <  1:2776707/61‑1 (MQ=255)             cgGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATACGAAGGCACtgtttgt             <  1:3173775/61‑1 (MQ=255)                  tATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATACGAAGGCACTGTTTGTTGCTTc       <  1:2071283/62‑1 (MQ=255)                  tATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATACGAAGGCACTGTTTGTTGCTTc       <  1:2730769/62‑1 (MQ=255)                  tATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATACGAAGGAACTGTTTGTTGCTTc       <  1:2575364/62‑1 (MQ=255)                        cTGGCAAAACAAATGCTGGCGCGGGAAGACTATACGAAGGCACTGTTTGTTGCTTCCGAtt  <  1:1819833/61‑1 (MQ=255)                        cTGGCAAAACAAATGCTGGCGCGGGAAGACTATACGAAGGCACTGTTTGTTGCTTCCGAtt  <  1:4421236/61‑1 (MQ=255)                        cTGGCAAAACAAATGCTGGCGCGGGAAGACTATACGAAGGCACTGTTTGTTGCTTCCGAtt  <  1:3416140/61‑1 (MQ=255)                           gCAAAACAAATGCTGGCGCGGGAAGACTATACGaa                         <  1:2944393/35‑1 (MQ=255)                           gCAAAACAAATGCTGGCGCGGGAAGACTATACGaa                         <  1:2104396/35‑1 (MQ=255)                           gCAAAACAAATGCTGGCGCGGGAAGACTATACGaa                         <  1:2061313/35‑1 (MQ=255)                           gCAAAACAAATGCTGGCGCGGGAAGACTATACGaa                         <  1:1343022/35‑1 (MQ=255)                           gCAAAACAAATGCTGGCGCGGGAAGACTATACGaa                         <  1:69813/35‑1 (MQ=255)                                aCAAATGCTGGCGCGGGAAGACTATACGAAGGCACtgtttgt             <  1:686106/42‑1 (MQ=255)                                       cTGGCGCGGGAAGACTATACGAAGGCACTGTTTGTTGCTTcc      >  1:3948300/1‑42 (MQ=255)                                       cTGGCGCGGGAAGACTATACGAAGGCACTGTTTGTTGCTTcc      >  1:4178308/1‑42 (MQ=255)                                       cTGGCGCGGGAAGACTATACGAAGGCACTGTTTGTTGCTTcc      >  1:908260/1‑42 (MQ=255)                                       cTGGCGCGGGAAGACTATACGAAGGCACTGTTTGTTGCTTcc      >  1:963434/1‑42 (MQ=255)                                                         |                            TTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATT  >  W3110S.gb/3220768‑3220851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑