Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,244,017	G→A	Q86Q (CAG→CAA)	exuT →	hexuronate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,244,017	0	G	A	100.0%	310.0 / NA	91	Q86Q (CAG→CAA)	exuT	hexuronate transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/66);  total (25/66)

AACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGT  >  W3110S.gb/3243958‑3244070                                                            |                                                      aaCACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAATa                                                     >  1:375306/1‑62 (MQ=255) aaCACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAATa                                                     >  1:3395274/1‑62 (MQ=255) aaCACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAATa                                                     >  1:2181617/1‑62 (MQ=255) aaCACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAATa                                                     >  1:1967758/1‑62 (MQ=255)               aGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAATATTCCTATATCATCg                                       <  1:435567/62‑1 (MQ=255)               aGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAATATTCCTATATCATCg                                       <  1:2311416/62‑1 (MQ=255)               aGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAATATTCCTATATCATCg                                       <  1:4229107/62‑1 (MQ=255)                   gcgcCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAATATTCCTATATCATCGCAGc                                   <  1:4524256/62‑1 (MQ=255)                   gcgcCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAATATTCCTATATCATCGCAGc                                   <  1:3575723/62‑1 (MQ=255)                   gcgcCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAATATTCCTATATCATCGCAGc                                   <  1:30002/62‑1 (MQ=255)                         aCTCTGATGGAAGAGTTAAACATCTCCACCCAACAATATTCCTATATCATCGCAGCCTATTc                             <  1:2701347/62‑1 (MQ=255)                          ctctGATGGAAGAGTTAAACATCTCCACCCAACAATATTCCTATATCATCGCAGCCTATTct                            >  1:40026/1‑62 (MQ=255)                           tctGATGGAAGAGTTAAACATCTCCACCCAACAATATTCCTATATCATCGCAGCCTATTctg                           <  1:877757/62‑1 (MQ=255)                                       gTTAAACATCTCCACCCAACAATATTCCTATATCATCGCAGCCTATTCtgctgc                       <  1:4589381/54‑1 (MQ=255)                                       gTTAAACATCTCCACCCAACAATATTCCTATATCATCGCAGCCTATTCtgctgc                       <  1:963439/54‑1 (MQ=255)                                       gTTAAACATCTCCACCCAACAATATTCCTATATCATCGCAGCCTATTCtgctgc                       <  1:503296/54‑1 (MQ=255)                                       gTTAAACATCTCCACCCAACAATATTCCTATATCATCGCAGCCTATTCtgctgc                       <  1:2396160/54‑1 (MQ=255)                                       gTTAAACATCTCCACCCAACAATATTCCTATATCATCGCAGCCTATTCtgctgc                       <  1:3961189/54‑1 (MQ=255)                                        ttAAACATCTCCACCCAACAATATTCCTATATCATCGCAg                                    <  1:3001963/40‑1 (MQ=255)                                        ttAAACATCTCCACCCAACAATATTCCTATATCATCGCAg                                    <  1:4623900/40‑1 (MQ=255)                                        ttAAACATCTCCACCCAACAATATTCCTATATCATCGCAg                                    <  1:2912676/40‑1 (MQ=255)                                        ttAAACATCTCCACCCAACAATATTCCTATATCATCGCAg                                    <  1:4689218/40‑1 (MQ=255)                                        ttAAACATCTCCACCCAACAATATTCCTATATCATCGCAg                                    <  1:4276677/40‑1 (MQ=255)                                        ttAAACATCTCCACCCAACAATATTCCTATATCATCGCAg                                    <  1:3341698/40‑1 (MQ=255)                                        ttAAACATCTCCACCCAACAATATTCCTATATCATCGCAg                                    <  1:3874711/40‑1 (MQ=255)                                        ttAAACATCTCCACCCAACAATATTCCTATATCATCGCAg                                    <  1:2428146/40‑1 (MQ=255)                                        ttAAACATCTCCACCCAACAATATTCCTATATCATCGCAg                                    <  1:967844/40‑1 (MQ=255)                                        ttAAACATCTCCACCCAACAATATTCCTATATCATCGCAg                                    <  1:3531044/40‑1 (MQ=255)                                        ttAAACATCTCCACCCAACAATATTCCTATATCATCGCAg                                    <  1:2370131/40‑1 (MQ=255)                                        ttAAACATCTCCACCCAACAATATTCCTATATCATCGCAg                                    <  1:3662526/40‑1 (MQ=255)                                        ttAAACATCTCCACCCAACAATATTCCTATATCATCGCAg                                    <  1:2299421/40‑1 (MQ=255)                                        ttAAACATCTCCACCCAACAATATTCCTATATCATCGCAg                                    <  1:223945/40‑1 (MQ=255)                                        ttAAACATCTCCACCCAACAATATTCCTATATCATCGCAg                                    <  1:2053988/40‑1 (MQ=255)                                        ttAAACATCTCCACCCAACAATATTCCTATATCATCGCAg                                    <  1:1981476/40‑1 (MQ=255)                                        ttAAACATCTCCACCCAACAATATTCCTATATCATCGCAg                                    <  1:962387/40‑1 (MQ=255)                                        ttAAACATCTCCACCCAACAATATTCCTATATCATCGCAg                                    <  1:1957690/40‑1 (MQ=255)                                        ttAAACATCTCCACCCAACAATATTCCTATATCATCGCAg                                    <  1:1884954/40‑1 (MQ=255)                                         tAAACATCTCCACCCAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTc             >  1:3529200/1‑62 (MQ=255)                                         tAAACATCTCCACCCAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTc             >  1:4236933/1‑62 (MQ=255)                                         tAAACATCTCCACCCAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTc             >  1:1931743/1‑62 (MQ=255)                                              aTCTCCACCCAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCtt                     <  1:4150765/49‑1 (MQ=255)                                              aTCTCCACCCAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCtt                     <  1:494556/49‑1 (MQ=255)                                              aTCTCCACCCAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCtt                     <  1:643909/49‑1 (MQ=255)                                              aTCTCCACCCAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCtt                     <  1:2672495/49‑1 (MQ=255)                                              aTCTCCACCCAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCtt                     <  1:686719/49‑1 (MQ=255)                                              aTCTCCACCCAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCtt                     <  1:2551798/49‑1 (MQ=255)                                              aTCTCCACCCAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCtt                     <  1:708078/49‑1 (MQ=255)                                              aTCTCCACCCAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCtt                     <  1:180705/49‑1 (MQ=255)                                              aTCTCCACCCAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCtt                     <  1:140534/49‑1 (MQ=255)                                              aTCTCCACCCAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCtt                     <  1:999618/49‑1 (MQ=255)                                              aTCTCCACCCAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACg                >  1:3562309/1‑54 (MQ=255)                                              aTCTCCACCCAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACg                >  1:3936547/1‑54 (MQ=255)                                              aTCTCCACCCAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACg                >  1:4622299/1‑54 (MQ=255)                                              aTCTCCACCCAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACg                >  1:654979/1‑54 (MQ=255)                                              aTCTCCACCCAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACg                >  1:670340/1‑54 (MQ=255)                                              aTCTCCACCCAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACg                >  1:865694/1‑54 (MQ=255)                                              aTCTCCACCCAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACg                >  1:1301399/1‑54 (MQ=255)                                              aTCTCCACCCAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACg                >  1:2746293/1‑54 (MQ=255)                                              aTCTCCACCCAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACg                >  1:2763949/1‑54 (MQ=255)                                              aTCTCCACCCAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACg                >  1:320587/1‑54 (MQ=255)                                                ctcCACCCAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCtt                     <  1:3830429/47‑1 (MQ=255)                                                ctcCACCCAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCtt                     <  1:132538/47‑1 (MQ=255)                                                ctcCACCCAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCtt                     <  1:146021/47‑1 (MQ=255)                                                ctcCACCCAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCtt                     <  1:2009392/47‑1 (MQ=255)                                                ctcCACCCAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCtt                     <  1:4706404/47‑1 (MQ=255)                                                ctcCACCCAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCtt                     <  1:2865994/47‑1 (MQ=255)                                                 tcCACCCAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCtt                     <  1:1322820/46‑1 (MQ=255)                                                 tcCACCCAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCtt                     <  1:3928703/46‑1 (MQ=255)                                                 tcCACCCAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCtt                     <  1:993150/46‑1 (MQ=255)                                                 tcCACCCAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCtt                     <  1:1782518/46‑1 (MQ=255)                                                 tcCACCCAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCtt                     <  1:2157172/46‑1 (MQ=255)                                                 tcCACCCAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCtt                     <  1:4707911/46‑1 (MQ=255)                                                 tcCACCCAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCtt                     <  1:3009457/46‑1 (MQ=255)                                                 tcCACCCAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCtt                     <  1:322212/46‑1 (MQ=255)                                                 tcCACCCAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCtt                     <  1:4451170/46‑1 (MQ=255)                                                 tcCACCCAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCtt                     <  1:4193077/46‑1 (MQ=255)                                                 tcCACCCAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCtt                     <  1:3602508/46‑1 (MQ=255)                                                 tcCACCCAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCtt                     <  1:439746/46‑1 (MQ=255)                                                 tcCACCCAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCtt                     <  1:3270711/46‑1 (MQ=255)                                                     cccAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGt              >  1:3061296/1‑49 (MQ=255)                                                     cccAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGt              >  1:4594777/1‑49 (MQ=255)                                                     cccAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGt              >  1:2759759/1‑49 (MQ=255)                                                     cccAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGt              >  1:1601905/1‑49 (MQ=255)                                                     cccAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGt              >  1:782291/1‑49 (MQ=255)                                                     cccAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGt              >  1:83788/1‑49 (MQ=255)                                                     cccAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGt  <  1:391076/61‑1 (MQ=255)                                                     cccAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGt  <  1:3772530/61‑1 (MQ=255)                                                     cccAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGt  <  1:3281245/61‑1 (MQ=255)                                                     cccAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGt  <  1:2614262/61‑1 (MQ=255)                                                     cccAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGt  <  1:2723682/61‑1 (MQ=255)                                                     cccAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCTTAAACGGt              >  1:707218/1‑49 (MQ=255)                                                       caacaaTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGt              >  1:2809323/1‑47 (MQ=255)                                                       caacaaTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGt              >  1:4566025/1‑47 (MQ=255)                                                            |                                                      AACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGT  >  W3110S.gb/3243958‑3244070

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑