Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,259,237	G→A	S613F (TCT→TTT)	tdcE ←	pyruvate formate‑lyase 4/2‑ketobutyrate formate‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,259,237	0	G	A	100.0%	90.4 / NA	28	S613F (TCT→TTT)	tdcE	pyruvate formate‑lyase 4/2‑ketobutyrate formate‑lyase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/16);  total (12/16)

GACGACCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGTTGGCAGCGCTTT  >  W3110S.gb/3259190‑3259295                                                |                                                           gacgacCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGa                                              >  1:1313497/1‑62 (MQ=255)    gacTGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATc                                           <  1:3327063/62‑1 (MQ=255)    gacCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTc                                                 <  1:2704736/56‑1 (MQ=255)    gacCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTc                                                 <  1:3503958/56‑1 (MQ=255)    gacCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATc                                           <  1:464111/62‑1 (MQ=255)    gacCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATc                                           <  1:1316859/62‑1 (MQ=255)    gacCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATc                                           <  1:4398707/62‑1 (MQ=255)    gacCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATc                                           <  1:1821338/62‑1 (MQ=255)    gacCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATc                                           <  1:1952005/62‑1 (MQ=255)    gacCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATc                                           <  1:3728948/62‑1 (MQ=255)    gacCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATc                                           <  1:2469608/62‑1 (MQ=255)    gacCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATc                                           <  1:2568726/62‑1 (MQ=255)    gacCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATc                                           <  1:2918369/62‑1 (MQ=255)     acCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATc                                           <  1:2881487/61‑1 (MQ=255)     acCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATc                                           <  1:3960259/61‑1 (MQ=255)        gTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATCGACt                                       <  1:3078820/62‑1 (MQ=255)        gTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATCGACt                                       <  1:3626361/62‑1 (MQ=255)           cGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATCg                                          >  1:3882362/1‑56 (MQ=255)           cGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATCg                                          >  1:818034/1‑56 (MQ=255)           cGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATCg                                          >  1:4303528/1‑56 (MQ=255)           cGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATCg                                          >  1:1740700/1‑56 (MQ=255)           cGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATCg                                          >  1:1162317/1‑56 (MQ=255)           cGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATCGACtaga                                    >  1:2172347/1‑59 (MQ=255)                  ttACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATCGACTGGGTAGGGAc                             >  1:1167373/1‑62 (MQ=255)                         ttttCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGtt                       >  1:4598436/1‑61 (MQ=255)                                             ttAAAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGTTGGCAGCGCttt  >  1:499377/1‑62 (MQ=255)                                             ttAAAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGTTGGCAGCGCttt  >  1:1846565/1‑62 (MQ=255)                                             ttAAAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGTTGGCAGCGCttt  >  1:994701/1‑62 (MQ=255)                                                |                                                           GACGACCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGTTGGCAGCGCTTT  >  W3110S.gb/3259190‑3259295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑