Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,294,438	T→C	L395S (TTG→TCG)	yraM →	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,294,438	0	T	C	100.0%	258.9 / NA	69	L395S (TTG→TCG)	yraM	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (42/27);  total (42/27)

CAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAA  >  W3110S.gb/3294383‑3294489                                                        |                                                    cagGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGa                                                <  1:3231144/61‑1 (MQ=255) cagGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGa                                                <  1:1817242/61‑1 (MQ=255) cagGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGa                                                <  1:2469384/61‑1 (MQ=255) cagGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGa                                                <  1:2407264/61‑1 (MQ=255) cagGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGa                                                <  1:3813022/61‑1 (MQ=255) cagGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGa                                                <  1:2097083/61‑1 (MQ=255)    gATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGTAGAGTCGCTGaa                                               >  1:2704779/1‑59 (MQ=255)    gATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAaga                                             >  1:2224341/1‑61 (MQ=255)    gATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAaga                                             >  1:2925433/1‑61 (MQ=255)    gATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAaga                                             >  1:2758054/1‑61 (MQ=255)    gATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAaga                                             >  1:3277038/1‑61 (MQ=255)    gATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAaga                                             >  1:2306996/1‑61 (MQ=255)    gATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAaga                                             >  1:3515011/1‑61 (MQ=255)    gATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAaga                                             >  1:3875462/1‑61 (MQ=255)    gATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAaga                                             >  1:1908172/1‑61 (MQ=255)    gATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAaga                                             >  1:4402612/1‑61 (MQ=255)    gATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAaga                                             >  1:17298/1‑61 (MQ=255)    gATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAaga                                             >  1:4565549/1‑61 (MQ=255)    gATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAaga                                             >  1:798627/1‑61 (MQ=255)     aTGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGc                                           <  1:2259767/62‑1 (MQ=255)     aTGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGc                                           <  1:3459830/62‑1 (MQ=255)     aTGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGc                                           <  1:4469659/62‑1 (MQ=255)     aTGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGc                                           <  1:3093224/62‑1 (MQ=255)             aGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTcc                                   >  1:940987/1‑62 (MQ=255)             aGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTcc                                   >  1:3373071/1‑62 (MQ=255)             aGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTcc                                   >  1:3241906/1‑62 (MQ=255)             aGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTcc                                   >  1:109499/1‑62 (MQ=255)             aGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTcc                                   >  1:2947528/1‑62 (MQ=255)             aGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTcc                                   >  1:2595860/1‑62 (MQ=255)                       tcggtcCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTa                         >  1:3701116/1‑62 (MQ=255)                          gtcCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACt                       >  1:3960333/1‑61 (MQ=255)                          gtcCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACt                       >  1:1840665/1‑61 (MQ=255)                           tcCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTgt                     <  1:1492270/62‑2 (MQ=255)                           tcCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTgg                     <  1:3313426/62‑1 (MQ=255)                           tcCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTgg                     <  1:2141259/62‑1 (MQ=255)                           tcCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTgg                     <  1:4039318/62‑1 (MQ=255)                           tcCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTgg                     <  1:4041923/62‑1 (MQ=255)                           tcCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTgg                     <  1:4366884/62‑1 (MQ=255)                           tcCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTgg                     <  1:4392502/62‑1 (MQ=255)                           tcCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTgg                     <  1:2676753/62‑1 (MQ=255)                           tcCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTgg                     <  1:1541284/62‑1 (MQ=255)                           tcCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTgg                     <  1:3076167/62‑1 (MQ=255)                           tcCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTgg                     <  1:521311/62‑1 (MQ=255)                            ccGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTgg                     <  1:3474867/61‑1 (MQ=255)                             cGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTgg                     <  1:3427540/60‑1 (MQ=255)                               ttGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAAc                            >  1:4224669/1‑51 (MQ=255)                               ttGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACg                           >  1:1967630/1‑52 (MQ=255)                               ttGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACg                           >  1:129103/1‑52 (MQ=255)                               ttGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACg                           >  1:3023361/1‑52 (MQ=255)                                tGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCaa                                         >  1:3830356/1‑37 (MQ=255)                                tGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCaa                                         >  1:4022396/1‑37 (MQ=255)                                tGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCaa                                         >  1:2363713/1‑37 (MQ=255)                                tGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCaa                                         >  1:2559984/1‑37 (MQ=255)                                tGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCaa                                         >  1:3214875/1‑37 (MQ=255)                                tGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCaa                                         >  1:1563485/1‑37 (MQ=255)                                tGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCaa                                         >  1:277474/1‑37 (MQ=255)                                 gCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGc                                 <  1:398001/44‑1 (MQ=255)                                 gCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGc                                 <  1:559377/44‑1 (MQ=255)                                        aaTAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGc         >  1:2964317/1‑61 (MQ=255)                                        aaTAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGc         >  1:2977622/1‑61 (MQ=255)                                        aaTAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGc         >  1:715953/1‑61 (MQ=255)                                        aaTAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGc         >  1:710445/1‑61 (MQ=255)                                        aaTAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGc         >  1:654198/1‑61 (MQ=255)                                        aaTAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGc         >  1:451568/1‑61 (MQ=255)                                        aaTAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGc         >  1:2616534/1‑61 (MQ=255)                                        aaTAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGc         >  1:3373836/1‑61 (MQ=255)                                        aaTAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGc         >  1:2027105/1‑61 (MQ=255)                                              gTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGaa  <  1:3393117/62‑1 (MQ=255)                                                tGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTgg                     <  1:594372/41‑1 (MQ=255)                                                        |                                                    CAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAA  >  W3110S.gb/3294383‑3294489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑