Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,306,434	G→A	L428L (CTG→TTG)	deaD ←	ATP‑dependent RNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,306,434	0	G	A	100.0%	96.2 / NA	30	L428L (CTG→TTG)	deaD	ATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/10);  total (20/10)

GCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTT  >  W3110S.gb/3306383‑3306491                                                    |                                                          gcgcATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGc                                                     >  1:2699259/1‑58 (MQ=255) gcgcATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCa                                                    >  1:3690660/1‑59 (MQ=255) gcgcATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCa                                                    >  1:3156513/1‑59 (MQ=255) gcgcATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCa                                                    >  1:2147325/1‑59 (MQ=255) gcgcATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCa                                                    >  1:1903275/1‑59 (MQ=255) gcgcATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCa                                                    >  1:3850897/1‑59 (MQ=255)       cTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGtt                                           >  1:3406339/1‑62 (MQ=255)       cTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGtt                                           >  1:2818845/1‑62 (MQ=255)       cTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGtt                                           >  1:1288133/1‑62 (MQ=255)       cTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGtt                                           >  1:4455262/1‑62 (MQ=255)            ggTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCga                                       <  1:1578121/61‑1 (MQ=255)            ggTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCga                                       <  1:1331982/61‑1 (MQ=255)            ggTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCga                                       <  1:3777174/61‑1 (MQ=255)            ggTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCga                                       <  1:3016454/61‑1 (MQ=255)            ggTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCga                                       <  1:3276033/61‑1 (MQ=255)             gTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCgaga                                     <  1:1721597/62‑1 (MQ=255)             gTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCgaga                                     <  1:1369465/62‑1 (MQ=255)             gTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCgaga                                     <  1:4156108/62‑1 (MQ=255)             gTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCgaga                                     <  1:3139593/62‑1 (MQ=255)                  aTCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCGAGATCCAg                                <  1:387525/62‑1 (MQ=255)                      gagTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCGAGATCCAGctc                             >  1:748494/1‑61 (MQ=255)                      gagTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCGAGATCCAGctc                             >  1:4328560/1‑61 (MQ=255)                      gagTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCGAGATCCAGctc                             >  1:996327/1‑61 (MQ=255)                      gagTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCGAGATCCAGctc                             >  1:1045097/1‑61 (MQ=255)                      gagTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCGAGATCCAGctc                             >  1:2344594/1‑61 (MQ=255)                      gagTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCGAGATCCAGctc                             >  1:1213259/1‑61 (MQ=255)                      gagTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCGAGATCCAGctc                             >  1:1102971/1‑61 (MQ=255)                         tACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTc                          >  1:1808428/1‑61 (MQ=255)                         tACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCa                         >  1:52463/1‑62 (MQ=255)                         tACGTTCACCCTGTGCCATTTTAAGCAATGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTc                          >  1:3850547/1‑61 (MQ=255)                                                agcaaTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAAttt   <  1:3333416/61‑1 (MQ=255)                                                agcaaTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAAttt   <  1:3795144/61‑1 (MQ=255)                                                agcaaTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAAttt   <  1:1902790/61‑1 (MQ=255)                                                agcaaTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAAttt   <  1:1346573/61‑1 (MQ=255)                                                agcaaTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAAttt   <  1:4015525/61‑1 (MQ=255)                                                agcaaTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAAttt   <  1:4667266/61‑1 (MQ=255)                                                agcaaTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAAttt   <  1:541389/61‑1 (MQ=255)                                                 gcaaTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAAtttt  <  1:3206314/61‑1 (MQ=255)                                                 gcaaTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAAtttt  <  1:3903526/61‑1 (MQ=255)                                                 gcaaTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAAtttt  <  1:1049738/61‑1 (MQ=255)                                                    |                                                          GCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTT  >  W3110S.gb/3306383‑3306491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑