| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,332,615 | T→A | intergenic (‑25/+102) | yhbE ← / ← rpmA | conserved inner membrane protein/50S ribosomal subunit protein L27 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,332,615 | 0 | T | A | 100.0% | 76.2 / NA | 24 | intergenic (‑25/+102) | yhbE/rpmA | conserved inner membrane protein/50S ribosomal subunit protein L27 |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (12/12); total (12/12) | |||||||||||
AATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCC > W3110S.gb/3332564‑3332670 | aatgccaatgccTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTAGCGGATTAAc < 1:3856195/62‑1 (MQ=255) ctTCATACCGTATTCCCTGCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTa > 1:2374848/1‑62 (MQ=255) ttCCCTGCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGaa > 1:3947885/1‑62 (MQ=255) ttCCCTGCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGaa > 1:2058345/1‑62 (MQ=255) ttCCCTGCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGaa > 1:2169864/1‑62 (MQ=255) ttCCCTGCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGaa > 1:2294650/1‑62 (MQ=255) ttCCCTGCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGaa > 1:1370048/1‑62 (MQ=255) gCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCgg < 1:2978202/48‑1 (MQ=255) gCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCgg < 1:99987/48‑1 (MQ=255) gCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCgg < 1:453081/48‑1 (MQ=255) gCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCgg < 1:427402/48‑1 (MQ=255) gCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCgg < 1:325759/48‑1 (MQ=255) gCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCgg < 1:1948865/48‑1 (MQ=255) gCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCgg < 1:1791797/48‑1 (MQ=255) gCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCgg < 1:1791416/48‑1 (MQ=255) gCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCgg < 1:1538077/48‑1 (MQ=255) gCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCgg < 1:1468236/48‑1 (MQ=255) gCAAAGCACATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCgg < 1:4637639/48‑1 (MQ=38) cAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTaaaa > 1:3591697/1‑62 (MQ=255) cAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTaaaa > 1:550538/1‑62 (MQ=255) cAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTaaaa > 1:62211/1‑62 (MQ=255) gcATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGccc > 1:2341281/1‑61 (MQ=255) gcATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGccc > 1:2021062/1‑61 (MQ=255) gcATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGccc > 1:394937/1‑61 (MQ=255) | AATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCC > W3110S.gb/3332564‑3332670 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |