Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,338,549	G→A	F185F (TTC→TTT)	yrbE ←	predicted toluene transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,338,549	0	G	A	100.0%	94.1 / NA	29	F185F (TTC→TTT)	yrbE	predicted toluene transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/18);  total (11/18)

TCAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAGATCC  >  W3110S.gb/3338494‑3338607                                                        |                                                           tCAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAaaagaa                                                       <  1:4453563/61‑1 (MQ=255) tCAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAaaagaa                                                       <  1:4342908/61‑1 (MQ=255) tCAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAaaagaa                                                       <  1:424567/61‑1 (MQ=255) tCAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAaaagaa                                                       <  1:2061142/61‑1 (MQ=255) tCAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAaaagaa                                                       <  1:2919246/61‑1 (MQ=255)  cAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAaaagaa                                                       <  1:622806/60‑1 (MQ=255)  cAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAaaagaa                                                       <  1:594991/60‑1 (MQ=255)  cAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAaaagaa                                                       <  1:2956066/60‑1 (MQ=255)  cAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAAAAGAAcc                                                     <  1:869837/62‑1 (MQ=255)  cAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAAAAGAAcc                                                     <  1:4083907/62‑1 (MQ=255)  cAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAAAAGAAcc                                                     <  1:3563311/62‑1 (MQ=255)  cAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAAAAGAAcc                                                     <  1:1417805/62‑1 (MQ=255)  cAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAAAAGAAcc                                                     <  1:2075617/62‑1 (MQ=255)  cAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAAAAGAAcc                                                     <  1:1931754/62‑1 (MQ=255)              cAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATg                                          <  1:216669/61‑1 (MQ=255)              cAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATg                                          <  1:2374078/61‑1 (MQ=255)              cAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATg                                          <  1:1681677/61‑1 (MQ=255)              cAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATg                                          <  1:2108541/61‑1 (MQ=255)              cAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATg                                          <  1:2709797/61‑1 (MQ=255)                 aTCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCtt                                      <  1:3604066/62‑1 (MQ=255)                   ccATACGCCAGTCGACGGCATTTTGCATTGCCGACCAAAAGAAcc                                                     <  1:587473/45‑1 (MQ=255)                   ccATACGCCAGTCGACGGCATTTTGCATTGCCGACCAAAAGAAcc                                                     <  1:243499/45‑1 (MQ=255)                          ccAGTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATc                                              >  1:1216932/1‑45 (MQ=255)                          ccAGTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATc                                              >  1:1223638/1‑45 (MQ=255)                          ccAGTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATc                                              >  1:1851807/1‑45 (MQ=255)                          ccAGTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATc                                              >  1:1249173/1‑45 (MQ=255)                          ccAGTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATc                                              >  1:3815121/1‑45 (MQ=255)                          ccAGTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATc                                              >  1:3941619/1‑45 (MQ=255)                           cAGTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATg                                          <  1:1133678/48‑1 (MQ=255)                                    gCATTTTGCATTGCCGACCAAAAGCACCCGCTATCAATGCCTTTCCAACTGCCGCCGAc                      >  1:3731011/1‑59 (MQ=255)                                    gCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGAc                      >  1:2642192/1‑59 (MQ=255)                                    gCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGAc                      >  1:3414740/1‑59 (MQ=255)                                    gCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGAc                      >  1:3356169/1‑59 (MQ=255)                                         ttGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGa                       <  1:1582220/53‑1 (MQ=255)                                         ttGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGa                       <  1:301383/53‑1 (MQ=255)                                                gCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGc           >  1:39004/1‑58 (MQ=255)                                                     ccAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAGATcc  <  1:2439438/62‑1 (MQ=255)                                                        |                                                           TCAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAGATCC  >  W3110S.gb/3338494‑3338607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑