Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	429,850	T→C	Y8H (TAT→CAT)	yajD →	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	429,850	0	T	C	100.0%	140.5 / NA	38	Y8H (TAT→CAT)	yajD	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/14);  total (24/14)

ATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTC  >  W3110S.gb/429808‑429906                                           |                                                         aTTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACCATGCGCGGTTAGAAAg                                          >  1:2394721/1‑59 (MQ=255) aTTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACCATGCGCGGTTAGAAAGCg                                        >  1:161036/1‑61 (MQ=255)   ttCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACCATGCGCGGTTAGAAAGCGGCt                                     >  1:2231719/1‑62 (MQ=255)    tCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACCATGCGCGGTTAGAAAGCGGCTa                                    <  1:4532137/62‑1 (MQ=255)    tCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACCATGCGCGGTTAGAAAGCGGCTa                                    <  1:483311/62‑1 (MQ=255)              aaCTTTTCATGGCTATCATCCCAAAAAACCATGCGCGGTTAGAAAGCGGCTATCGCGaaaaa                          <  1:925123/62‑1 (MQ=255)              aaCTTTTCATGGCTATCATCCCAAAAAACCATGCGCGGTTAGAAAGCGGCTATCGCGaaaaa                          <  1:4522913/62‑1 (MQ=255)              aaCTTTTCATGGCTATCATCCCAAAAAACCATGCGCGGTTAGAAAGCGGCTATCGCGaaaaa                          <  1:3527873/62‑1 (MQ=255)              aaCTTTTCATGGCTATCATCCCAAAAAACCATGCGCGGTTAGAAAGCGGCTATCGCGaaaaa                          <  1:1240014/62‑1 (MQ=255)              aaCTTTTCATGGCTATCATCCCAAAAAACCATGCGCGGTTAGAAAGCGGCTATCGCGaaaaa                          <  1:919649/62‑1 (MQ=255)                 ttttCATGGCTATCATCCCAAAAAACCATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGc                        <  1:2454083/61‑1 (MQ=255)                     cATGGCTATCATCCCAAAAAACCATGCGCGGTTAGAAAg                                          >  1:1561087/1‑39 (MQ=255)                     cATGGCTATCATCCCAAAAAACCATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTa                    <  1:678249/61‑1 (MQ=255)                     cATGGCTATCATCCCAAAAAACCATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTa                    >  1:4459984/1‑61 (MQ=255)                     cATGGCTATCATCCCAAAAAACCATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTa                    >  1:4628303/1‑61 (MQ=255)                     cATGGCTATCATCCCAAAAAACCATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTa                    >  1:755117/1‑61 (MQ=255)                     cATGGCTATCATCCCAAAAAACCATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTa                    <  1:1590380/61‑1 (MQ=255)                     cATGGCTATCATCCCAAAAAACCATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTa                    >  1:1954897/1‑61 (MQ=255)                           tATCATCCCAAAAAACCATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCt             <  1:2444199/62‑1 (MQ=255)                           tATCATCCCAAAAAACCATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCt             <  1:2629453/62‑1 (MQ=255)                           tATCATCCCAAAAAACCATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCt             <  1:3464545/62‑1 (MQ=255)                           tATCATCCCAAAAAACCATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCt             <  1:3702919/62‑1 (MQ=255)                                    aaaaaaCCATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTggg    >  1:56268/1‑62 (MQ=255)                                    aaaaaaCCATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTggg    >  1:574717/1‑62 (MQ=255)                                    aaaaaaCCATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTggg    >  1:1070722/1‑62 (MQ=255)                                    aaaaaaCCATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTggg    >  1:243557/1‑62 (MQ=255)                                      aaaaCCATGCGCGGTTAGAAAGCGGCTATCGCGAATAAGCATTAAAAATCTATCCGTGGGt   >  1:4604786/1‑61 (MQ=255)                                      aaaaCCATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGt   >  1:2343909/1‑61 (MQ=255)                                      aaaaCCATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTc  >  1:4470108/1‑62 (MQ=255)                                      aaaaCCATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTc  >  1:3612444/1‑62 (MQ=255)                                      aaaaCCATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTc  >  1:2924112/1‑62 (MQ=255)                                      aaaaCCATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTc  >  1:4624468/1‑62 (MQ=255)                                      aaaaCCATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTc  >  1:2921501/1‑62 (MQ=255)                                      aaaaCCATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTc  >  1:2735917/1‑62 (MQ=255)                                      aaaaCCATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTc  >  1:2208845/1‑62 (MQ=255)                                      aaaaCCATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTc  >  1:1580786/1‑62 (MQ=255)                                      aaaaCCATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTc  >  1:143059/1‑62 (MQ=255)                                      aaaaCCATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTc  >  1:1003549/1‑62 (MQ=255)                                           |                                                         ATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTC  >  W3110S.gb/429808‑429906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑