Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,375,433	A→G	D237G (GAT→GGT)	dcuD →	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,375,433	0	A	G	100.0%	125.2 / NA	34	D237G (GAT→GGT)	dcuD	predicted transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (3/31);  total (3/31)

AAAAAAGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAAT  >  W3110S.gb/3375384‑3375481                                                  |                                                 aaaaaaGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGGTAATGTCccgcc                                      <  1:773497/62‑1 (MQ=255) aaaaaaGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGGTAATGTCccgcc                                      <  1:4016693/62‑1 (MQ=255) aaaaaaGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGGTAATGTCccgcc                                      <  1:3901711/62‑1 (MQ=255) aaaaaaGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGGTAATGTCccgcc                                      <  1:3471775/62‑1 (MQ=255) aaaaaaGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGGTAATGTCccgcc                                      <  1:1118064/62‑1 (MQ=255)  aaaaaGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGGTAATGTCccgcc                                      <  1:194873/61‑1 (MQ=255)           aaaaTATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGGTAATGTCCCGCCGCTCTATTAc                            <  1:1308349/62‑1 (MQ=255)           aaaaTATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGGTAATGTCCCGCCGCTCTATTAc                            <  1:41309/62‑1 (MQ=255)           aaaaTATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGGTAATGTCCCGCCGCTCTATTAc                            <  1:3949789/62‑1 (MQ=255)           aaaaTATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGGTAATGTCCCGCCGCTCTATTAc                            <  1:2869538/62‑1 (MQ=255)           aaaaTATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGGTAATGTCCCGCCGCTCTATTAc                            <  1:254181/62‑1 (MQ=255)             aaTATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGGTAATGTCCCGCCGCTCTATTACGc                          <  1:3292641/62‑1 (MQ=255)             aaTATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGGTAATGTCCCGCCGCTCTATTACGc                          <  1:972298/62‑1 (MQ=255)             aaTATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGGTAATGTCCCGCCGCTCTATTACGc                          <  1:965895/62‑1 (MQ=255)             aaTATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGGTAATGTCCCGCCGCTCTATTACGc                          <  1:430997/62‑1 (MQ=255)             aaTATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGGTAATGTCCCGCCGCTCTATTACGc                          <  1:2844107/62‑1 (MQ=255)                   aatcaCGAACAGGCAGAGCAAAAAGCTCTCGGTAATGTCCCGCCGCTCTATTACGCCAttt                     <  1:1877525/61‑1 (MQ=255)                   aatcaCGAACAGGCAGAGCAAAAAGCTCTCGGTAATGTCCCGCCGCTCTATTACGCCAttt                     <  1:1158233/61‑1 (MQ=255)                   aatcaCGAACAGGCAGAGCAAAAAGCTCTCGGTAATGTCCCGCCGCTCTATTACGCCAttt                     <  1:1220212/61‑1 (MQ=255)                   aatcaCGAACAGGCAGAGCAAAAAGCTCTCGGTAATGTCCCGCCGCTCTATTACGCCAttt                     <  1:4592481/61‑1 (MQ=255)                   aatcaCGAACAGGCAGAGCAAAAAGCTCTCGGTAATGTCCCGCCGCTCTATTACGCCAttt                     <  1:4255607/61‑1 (MQ=255)                   aatcaCGAACAGGCAGAGCAAAAAGCTCTCGGTAATGTCCCGCCGCTCTATTACGCCAttt                     <  1:4185252/61‑1 (MQ=255)                   aatcaCGAACAGGCAGAGCAAAAAGCTCTCGGTAATGTCCCGCCGCTCTATTACGCCAttt                     <  1:3581503/61‑1 (MQ=255)                                 agagCAAAAAGCTCTCGGTAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGt      <  1:1882712/62‑1 (MQ=255)                                 agagCAAAAAGCTCTCGGTAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGt      <  1:34754/62‑1 (MQ=255)                                 agagCAAAAAGCTCTCGGTAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGt      <  1:2299139/62‑1 (MQ=255)                                 agagCAAAAAGCTCTCGGTAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGt      <  1:1211962/62‑1 (MQ=255)                                     cAAAAAGCTCTCGGTAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGt      <  1:1979992/58‑1 (MQ=255)                                     cAAAAAGCTCTCGGTAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAAt  >  1:1715151/1‑62 (MQ=255)                                     cAAAAAGCTCTCGGTAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAAt  >  1:224222/1‑62 (MQ=255)                                     cAAAAAGCTCTCGGTAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGAAGCCGTTAAt  >  1:171518/1‑62 (MQ=255)                                          aGCTCTCGGTAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGt      <  1:396327/53‑1 (MQ=255)                                          aGCTCTCGGTAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGt      <  1:4385747/53‑1 (MQ=255)                                          aGCTCTCGGTAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGt      <  1:2422198/53‑1 (MQ=255)                                                  |                                                 AAAAAAGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAAT  >  W3110S.gb/3375384‑3375481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑