Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,459,280	T→C	E15G (GAA→GGA)	secE ←	preprotein translocase membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,459,280	0	T	C	100.0%	244.2 / NA	65	E15G (GAA→GGA)	secE	preprotein translocase membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (46/19);  total (46/19)

GACAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAAACCAACCTGTCA  >  W3110S.gb/3459231‑3459337                                                  |                                                          gACAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCgcgc                                                <  1:999353/61‑1 (MQ=255)            ccaGGAGCAATGCCACCACAACGACCCACTTCATCGCTCCGAGGCCGCGCCCGCTTCCTTGa                                    <  1:841069/62‑1 (MQ=255)            ccaGGAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGa                                    <  1:1165553/62‑1 (MQ=255)            ccaGGAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGa                                    <  1:1660446/62‑1 (MQ=255)              aGGAGCAATGCCACCACAACGCCCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGc                                  >  1:3357248/1‑62 (MQ=255)              aGGAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGc                                  >  1:3069643/1‑62 (MQ=255)              aGGAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGc                                  >  1:4382686/1‑62 (MQ=255)              aGGAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGc                                  >  1:2208866/1‑62 (MQ=255)              aGGAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGc                                  <  1:2001333/62‑1 (MQ=255)              aGGAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGc                                  >  1:404787/1‑62 (MQ=255)              aGGAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGc                                  >  1:1403585/1‑62 (MQ=255)              aGGAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGc                                  >  1:613599/1‑62 (MQ=255)              aGGAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGc                                  >  1:2966125/1‑62 (MQ=255)               ggAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCtt                                          <  1:367451/53‑1 (MQ=255)               ggAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCtt                                          <  1:3974421/53‑1 (MQ=255)               ggAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCtt                                          <  1:2592524/53‑1 (MQ=255)               ggAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCtt                                          <  1:2046858/53‑1 (MQ=255)               ggAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCtt                                          <  1:2109504/53‑1 (MQ=255)               ggAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCtt                                          <  1:1611328/53‑1 (MQ=255)               ggAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCtt                                          <  1:1394043/53‑1 (MQ=255)               ggAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCtt                                          <  1:1009136/53‑1 (MQ=255)                gAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCtt                                          <  1:4609862/52‑1 (MQ=255)                 aGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCtt                                          <  1:4165673/51‑1 (MQ=255)                            cacaACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGa                                    <  1:1143046/46‑1 (MQ=255)                            cacaACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCa                     >  1:622897/1‑61 (MQ=255)                            cacaACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCa                     >  1:1955349/1‑61 (MQ=255)                              caACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACt                   >  1:2745349/1‑61 (MQ=255)                              caACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACt                   >  1:459326/1‑61 (MQ=255)                              caACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACt                   >  1:683151/1‑61 (MQ=255)                              caACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACt                   >  1:4595957/1‑61 (MQ=255)                              caACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACt                   >  1:3434987/1‑61 (MQ=255)                              caACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACt                   >  1:3408353/1‑61 (MQ=255)                              caACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACt                   >  1:2764738/1‑61 (MQ=255)                              caACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACt                   >  1:772296/1‑61 (MQ=255)                              caACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACt                   >  1:2629271/1‑61 (MQ=255)                              caACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACt                   >  1:2449942/1‑61 (MQ=255)                              caACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACt                   >  1:2249276/1‑61 (MQ=255)                              caACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACt                   >  1:2177808/1‑61 (MQ=255)                              caAAGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACt                   >  1:3909408/1‑61 (MQ=255)                               aaCGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTc                  >  1:2391785/1‑61 (MQ=255)                               aaCGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTc                  >  1:59529/1‑61 (MQ=255)                               aaCGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTc                  >  1:1215967/1‑61 (MQ=255)                               aaCGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTc                  >  1:1733287/1‑61 (MQ=255)                               aaCGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTc                  >  1:1761575/1‑61 (MQ=255)                               aaCGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTc                  >  1:2031860/1‑61 (MQ=255)                               aaCGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTc                  >  1:4517589/1‑61 (MQ=255)                               aaCGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTc                  >  1:4465054/1‑61 (MQ=255)                               aaCGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTc                  >  1:699493/1‑61 (MQ=255)                               aaCGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTc                  >  1:4093242/1‑61 (MQ=255)                               aaCGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTc                  >  1:4065129/1‑61 (MQ=255)                               aaCGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTc                  >  1:3966802/1‑61 (MQ=255)                                aCGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCa                 >  1:789933/1‑61 (MQ=255)                                aCGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCa                 >  1:1071127/1‑61 (MQ=255)                                aCGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCa                 >  1:71763/1‑61 (MQ=255)                                aCGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCa                 >  1:536398/1‑61 (MQ=255)                                aCGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCa                 >  1:4378425/1‑61 (MQ=255)                                aCGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCa                 >  1:2145726/1‑61 (MQ=255)                                aCGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCa                 >  1:1215477/1‑61 (MQ=255)                                       aCTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAaaccaa         >  1:464856/1‑62 (MQ=255)                                       aCTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAaaccaa         >  1:4559093/1‑62 (MQ=255)                                       aCTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAaacca          >  1:269497/1‑61 (MQ=255)                                       aCTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCATCGCTATTCGCACTCATAaaccaa         >  1:804862/1‑62 (MQ=255)                                              cGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAAACCAACCTGTCa  <  1:2848979/62‑1 (MQ=255)                                              cGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGATATTCGCACTCATAAACCAACCTGTCa  <  1:1666156/62‑1 (MQ=255)                                               gCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAAACCAACCTGTCa  <  1:3148917/61‑1 (MQ=255)                                                  |                                                          GACAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAAACCAACCTGTCA  >  W3110S.gb/3459231‑3459337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑