Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,472,611	G→A	G144G (GGC→GGT)	btuB ←	vitamin B12/cobalamin outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,472,611	0	G	A	100.0%	99.0 / NA	33	G144G (GGC→GGT)	btuB	vitamin B12/cobalamin outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/17);  total (16/17)

ATTTCCGTTCCGGGTTCATCGCGCGTCGTGATGATATTCACCACCCCGCCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGATATATTCAACAC  >  W3110S.gb/3472564‑3472666                                                |                                                        aTTTCCGTTCCGGGTTCATCGCGCGTCGTGATGATATTCACCACCCCACCTATTGCATCgg                                            <  1:811975/61‑1 (MQ=255) aTTTCCGTTCCGGGTTCATCGCGCGTCGTGATGATATTCACCACCCCACCTATTGCATCgg                                            <  1:3016030/61‑1 (MQ=255) aTTTCCGTTCCGGGTTCATCGCGCGTCGTGATGATATTCACCACCCCACCTATTGCATCgg                                            <  1:1003343/61‑1 (MQ=255) aTTTCCGTTCCGGGTTCATCGCGCGTCGTGATGATATTCACCACCCCACCTATTGCATCgg                                            <  1:1755707/61‑1 (MQ=255)        ttccgGGTTCATCGCGCGTCGTGATGATATTCACCACCCCACCTATTGCATCGGAACCATaa                                    <  1:1791121/62‑1 (MQ=255)        ttccgGGTTCATCGCGCGTCGTGATGATATTCACCACCCCACCTATTGCATCGGAACCATaa                                    <  1:3125168/62‑1 (MQ=255)        ttccgGGTTCATCGCGCGTCGTGATGATATTCACCACCCCACCTATTGCATCGGAACCATaa                                    <  1:2953143/62‑1 (MQ=255)        ttccgGGTTCATCGCGCGTCGTGATGATATTCACCACCCCACCTATTGCATCGGAACCATaa                                    <  1:2788811/62‑1 (MQ=255)        ttccgGGTTCATCGCGCGTCGTGATGATATTCACCACCCCACCTATTGCATCGGAACCATaa                                    <  1:1715990/62‑1 (MQ=255)        ttccgGGTTCATCGCGCGTCGTGATGATATTCACCACCCCACCTATTGCATCGGAACCATaa                                    <  1:3361839/62‑1 (MQ=255)        ttccgGGTTCATCGCGCGTCGTGATGATATTCACCACCCCACCTATTGCATCGGAACCATaa                                    <  1:3459955/62‑1 (MQ=255)        ttccgGGTTCATCGCGCGTCGTGATGATATTCACAACCCCACCTATTGCATCGGAACCATaa                                    <  1:3267933/62‑1 (MQ=255)                 cATCGCGCGTCGTGATGATATTCACCACCCCACCTATTGCATCGGAACCATAAACAGCGGAg                           <  1:1494184/62‑1 (MQ=255)                       gcgTCGTGATGATATTCACCACCCCACCTATTGCATCGGAACCATGAACAGCGGAGCGCgg                      >  1:2758102/1‑61 (MQ=39)                       gcgTCGTGATGATATTCACCACCCCACCTATTGCATCGGAACCATAAACAGCGGAGCGCgg                      >  1:1773197/1‑61 (MQ=255)                       gcgTCGTGATGATATTCACCACCCCACCTATTGCATCGGAACCATAAACAGCGGAGCGCgg                      >  1:2490348/1‑61 (MQ=255)                       gcgTCGTGATGATATTCACCACCCCACCTATTGCATCGGAACCATAAACAGCGGAGCGCgg                      >  1:578352/1‑61 (MQ=255)                       gcgTCGTGATGATATTCACCACCCCACCTATTGCATCGGAACCATAAACAGCGGAGCGCgg                      >  1:420226/1‑61 (MQ=255)                       gcgTCGTGATGATATTCACCACCCCACCTATTGCATCGGAACCATAAACAGCGGAGCGCgg                      >  1:148181/1‑61 (MQ=255)                       gcgTCGTGATGATATTCACCACCCCACCTATTGCATCGGAACCATAAACAG‑GGAGCGCgg                      >  1:1933557/1‑60 (MQ=38)                         gtcgtGATGATATTCACCACCCCACCTATTGCATCGGAACCATAAACAGCGGAGCGCGGccc                   <  1:3450393/62‑1 (MQ=255)                         gtcgtGATGATATTCACCACCCCACCTATTGCATCGGAACCATAAACAGCGGAGCGCGGccc                   <  1:4418966/62‑1 (MQ=255)                               atgatATTCACCACCCCACCTATTGCATCGGAACCATaa                                    <  1:1556493/39‑1 (MQ=255)                                   tatTCACCACCCCACCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGatata          >  1:904882/1‑61 (MQ=255)                                      tCACCACCCCACCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGa              >  1:2840880/1‑54 (MQ=255)                                      tCACCACCCCACCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGa              >  1:3961390/1‑54 (MQ=255)                                      tCACCACCCCACCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGa              >  1:3859680/1‑54 (MQ=255)                                      tCACCACCCCACCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGa              >  1:2989746/1‑54 (MQ=255)                                      tCACCACCCCACCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGa              >  1:1527469/1‑54 (MQ=255)                                      tCACCACCCCACCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGa              >  1:1198426/1‑54 (MQ=255)                                      tCACCACCCCACCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGa              >  1:1172224/1‑54 (MQ=255)                                       caccacCCCACCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGATATATTCaa     <  1:469863/62‑1 (MQ=255)                                            ccccACCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGATATATTCAacac  >  1:2138545/1‑60 (MQ=255)                                                |                                                        ATTTCCGTTCCGGGTTCATCGCGCGTCGTGATGATATTCACCACCCCGCCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGATATATTCAACAC  >  W3110S.gb/3472564‑3472666

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑