Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,497,165	G→A	A65T (GCG→ACG)	fsaB →	fructose‑6‑phosphate aldolase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,497,165	0	G	A	100.0%	103.7 / NA	32	A65T (GCG→ACG)	fsaB	fructose‑6‑phosphate aldolase 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/21);  total (11/21)

CTGCAAAAAGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGACGCTATTCCGGG  >  W3110S.gb/3497108‑3497214                                                          |                                                  cTGCAAAAAGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACACGCa                                               <  1:1130722/62‑1 (MQ=255) cTGCAAAAAGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACACGCa                                               <  1:2950820/62‑1 (MQ=255) cTGCAAAAAGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACACGCa                                               <  1:1565439/62‑1 (MQ=255)  tGCAAAAAGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACACGCa                                               <  1:2893999/61‑1 (MQ=255)              ttGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACACGCAGGGGATGGTGgaa                                  <  1:3935060/62‑1 (MQ=255)              ttGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACACGCAGGGGATGGTGgaa                                  <  1:4110366/62‑1 (MQ=255)              ttGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACACGCAGGGGATGGTGgaa                                  <  1:3292203/62‑1 (MQ=255)              ttGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACACGCAGGGGATGGTGgaa                                  <  1:2314395/62‑1 (MQ=255)              ttGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACACGCAGGGGATGGTGgaa                                  <  1:614272/62‑1 (MQ=255)               tGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACACGCAGGGGATGGTGgaa                                  <  1:2842385/61‑1 (MQ=255)                  tgatgaGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACACGCAGGGGATGGTGgaagaa                               <  1:3024587/61‑1 (MQ=255)                  tgatgaGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACACGCAGGGGATGGTGgaagaa                               <  1:531606/61‑1 (MQ=255)                  tgatgaGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACACGCAGGGGATGGTGgaagaa                               <  1:4482996/61‑1 (MQ=255)                  tgatgaGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACACGCAGGGGATGGTGgaagaa                               <  1:1096986/61‑1 (MQ=255)                    atgaGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACACTCAGGGGATGGTGGAAgaagcg                            <  1:1355993/62‑1 (MQ=255)                    atgaGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACACGCAGGGGATGGTGGAAgaagcg                            <  1:425463/62‑1 (MQ=255)                    atgaGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACACGCAGGGGATGGTGGAAgaagcg                            <  1:2013252/62‑1 (MQ=255)                    atgaGGGCATTCCGTTTGCTCAGACCATGAGCCGCGACACGCAGGGGATGGTGGAAgaagcg                            <  1:4279684/62‑1 (MQ=255)                                tGTTTGCTCAGACCATGAGCCGCGACACGCAGGGGATGGTGGAAGAAGCGAAGCGCCTgcgc                >  1:706311/1‑62 (MQ=255)                                tGTTTGCTCAGACCATGAGCCGCGACACGCAGGGGATGGTGGAAGAAGCGAAGCGCCTgcgc                >  1:830269/1‑62 (MQ=255)                                tGTTTGCTCAGACCATGAGCCGCGACACGCAGGGGATGGTGGAAGAAGCGAAGCGCCTgcgc                >  1:94802/1‑62 (MQ=255)                                tGTTTGCTCAGACCATGAGCCGCGACACGCAGGGGATGGTGGAAGAAGCGAAGCGCCTgcgc                >  1:3962342/1‑62 (MQ=255)                                tGTTTGCTCAGACCATGAGCCGCGACACGCAGGGGATGGTGGAAGAAGCGAAGCGCCTgcgc                >  1:2391377/1‑62 (MQ=255)                                tGTTTGCTCAGACCATGAGCCGCGACACGCAGGGGATGGTGGAAGAAGCGAAGCGCCTgcgc                >  1:2345916/1‑62 (MQ=255)                                tGTTTGCTCAGACCATGAGCCGCGACACGCAGGGGATGGTGGAAGAAGCGAAGCGCCTgcgc                >  1:1882420/1‑62 (MQ=255)                                      cTCAGACCATGAGCCGCGCCACGCAGGGGATGGTGGAAGAAGCGAAGCGCCTgcgc                >  1:751221/1‑56 (MQ=255)                                      cTCAGACCATGAGCCGCGACACGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGACGCt          >  1:1734921/1‑62 (MQ=255)                                      cTCAGACCATGAGCCGCGACACGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGACGCt          >  1:1611165/1‑62 (MQ=255)                                         aGACCATGAGCCGCGACACGCAGGGGATGGTGgaagaa                               <  1:387657/38‑1 (MQ=255)                                           aCCATGAGCCGCGACACGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGACGCTATTcc     >  1:2904977/1‑62 (MQ=255)                                                 aGCCGCGACACGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGACGCTATTCCggg  <  1:2639624/59‑1 (MQ=255)                                                  gCCGCGACACGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGACGCTATTCCggg  <  1:2452166/58‑1 (MQ=255)                                                          |                                                  CTGCAAAAAGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGACGCTATTCCGGG  >  W3110S.gb/3497108‑3497214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑