Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,516,285	T→C	intergenic (+20/‑47)	hslU → / → menA	molecular chaperone and ATPase component of HslUV protease/1,4‑dihydroxy‑2‑naphthoate octaprenyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,516,285	0	T	C	100.0%	111.7 / NA	31	intergenic (+20/‑47)	hslU/menA	molecular chaperone and ATPase component of HslUV protease/1,4‑dihydroxy‑2‑naphthoate octaprenyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/9);  total (22/9)

GTTGGTGGCAGATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGTATTATG  >  W3110S.gb/3516226‑3516334                                                            |                                                  gTTGGTGGCAGATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTtcacca                                                 >  1:783295/1‑62 (MQ=255)  ttGGTGGCAGATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTtcacca                                                 <  1:1564965/61‑1 (MQ=255)  ttGGTGGCAGATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTtcacca                                                 <  1:2348539/61‑1 (MQ=255)  ttGGTGGCAGATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTtcacca                                                 <  1:2161743/61‑1 (MQ=255)  ttGGTGGCAGATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTcaccat                                                <  1:1142350/62‑1 (MQ=255)  ttGGTGGCAGATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTcaccat                                                <  1:4278214/62‑1 (MQ=255)  ttGGTGGCAGATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTcaccat                                                <  1:4405137/62‑1 (MQ=255)  ttGGTGGCAGATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTcaccat                                                <  1:1029060/62‑1 (MQ=255)  ttGGTGGCAGATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTcaccat                                                <  1:2843816/62‑1 (MQ=255)  ttGGTGGCAGATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTcaccat                                                <  1:50657/62‑1 (MQ=255)  ttGGTGGCAGATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTcaccat                                                <  1:1959967/62‑1 (MQ=255)   tggtggCAGATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTcaccat                                                <  1:1510184/61‑1 (MQ=255)    ggtggCAGATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCACCAtt                                               <  1:2056301/61‑1 (MQ=255)           gATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCACCATTGTTTGATg                                       <  1:501050/62‑1 (MQ=255)           gATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCACCATTGTTTGATg                                       <  1:4357075/62‑1 (MQ=255)            aTGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCACCATTGTTTGATg                                       <  1:3658795/61‑1 (MQ=255)             tGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCACCATTGTTTGAt                                        <  1:595052/59‑1 (MQ=255)                 gATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCACCATTGTTTGATGGGGCt                                  >  1:3906340/1‑61 (MQ=255)                  aTCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCACCATTGTTTGATGGGGCTGa                                <  1:2955090/62‑1 (MQ=255)                    cTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCACCATTGTTTGAt                                        >  1:452380/1‑52 (MQ=255)                    cTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCACCATTGTTTGATGGGGCTGaaa                              >  1:2322095/1‑62 (MQ=255)                       aGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCACCATTGTTTGATGGGGCTGAAAgg                            >  1:3653919/1‑61 (MQ=255)                       aGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCACCATTGTTTGATGGGGCTGAAAGGc                           >  1:1577904/1‑62 (MQ=255)                       aGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCACCATTGTTTGATGGGGCTGAAAGGc                           >  1:292868/1‑62 (MQ=255)                       aGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCACCATTGTTTGATGGGGCTGAAAGGc                           >  1:4545796/1‑62 (MQ=255)                       aGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCACCATTGTTTGATGGGGCTGAAAGGc                           >  1:1156586/1‑62 (MQ=255)                       aGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCACCATTGTTTGATGGGGCTGAAAGGc                           >  1:1603525/1‑62 (MQ=255)                       aGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCACCATTGTTTGATGGGGCTGAAAGGc                           >  1:3601253/1‑62 (MQ=255)                       aGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCACCATTGTTTGATGGGGCTGAAAGGc                           >  1:3725007/1‑62 (MQ=255)                       aGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCACCATTGTTTGATGGGGCTGAAAGGc                           >  1:1828008/1‑62 (MQ=255)                       aGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCACCATTGTTTGATGGGGCTGAAAGGc                           >  1:4050721/1‑62 (MQ=255)                           gTTTTATCCTATAATCGCGTTCAATCATTTTCACCATTGTTTGaa                                        >  1:2988614/1‑44 (MQ=255)                           gTTTTATCCTATAATCGCGTTCAATCATTTTCACCATTGTTTGATg                                       <  1:2519743/46‑1 (MQ=255)                           gTTTTATCCTATAATCGCGTTCAATCATTTTCACCATTGTTTGATGGGGCTGAAAGGCCCCa                       >  1:4346663/1‑62 (MQ=255)                           gTTTTATCCTATAATCGCGTTCAATCATTTTCACCATTGTTTGATGGGGCTGAAAGGCCCCa                       >  1:1368864/1‑62 (MQ=255)                            ttttATCCTATAATCGCGTTCAATCATTTTcaccat                                                <  1:3532946/36‑1 (MQ=255)                                   cTATAATCGCGTTCAATCATTTTCACCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTAt                <  1:483460/61‑1 (MQ=255)                                     ataATCGCGTTCAATCATTTTCACCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTgg             <  1:2508917/62‑1 (MQ=255)                                             gTTCAATCATTTTCACCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGtat      >  1:2034205/1‑61 (MQ=255)                                             gTTCAATCATTTTCACCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGtat      >  1:99058/1‑61 (MQ=255)                                                  aacATTTTCACCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTgg             >  1:2333629/3‑49 (MQ=39)                                                  aTCATTTTCTCCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGtattat   >  1:371291/1‑59 (MQ=255)                                                  aTCATTTTCACCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGtattat   >  1:2152025/1‑59 (MQ=255)                                                  aTCATTTTCACCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGTATTATg  >  1:3286548/1‑60 (MQ=255)                                                            |                                                  GTTGGTGGCAGATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGTATTATG  >  W3110S.gb/3516226‑3516334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑