Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,584,576	G→A	P21S (CCA→TCA)	hemN ←	coproporphyrinogen III oxidase, SAM and NAD(P)H dependent, oxygen‑independent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,584,576	0	G	A	100.0%	106.5 / NA	33	P21S (CCA→TCA)	hemN	coproporphyrinogen III oxidase, SAM and NAD(P)H dependent, oxygen‑independent
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/17);  total (16/17)

TGTTCGCCGAAGTCTTCTGAAAACTCCAGCGCGGTCGGGTACGAGGTGTATCGTGGCCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAGTCGATTTGCTGT  >  W3110S.gb/3584521‑3584628                                                        |                                                     tGTTCGCCGAAGTCTTCTGAAAACTCCAGCGCGGTCGGGTACGAGGTGTATCGTGACCCGGa                                                <  1:2278035/62‑1 (MQ=255)            gTCTTCTGAAAACTCCAGCGCGGTCGGGTACGAGGTGTATCGTGACCCGGAATAGTTATAtt                                     >  1:3107619/1‑62 (MQ=255)                  tGAAAACTCCAGCGCGGTCGGGTACGAGGTGTATCGTGACCCGGAATAGTTATATTTCTg                                 >  1:1641531/1‑60 (MQ=255)                  tGAAAACTCCAGCGCGGTCGGGTACGAGGTGTATCGTGACCCGGAATAGTTATATTTCTg                                 >  1:360499/1‑60 (MQ=255)                  tGAAAACTCCAGCGCGGTCGGGTACGAGGTGTATCGTGACCCGGAATAGTTATATTTCTg                                 >  1:1836930/1‑60 (MQ=255)                  tGAAAACTCCAGCGCGGTCGGGTACGAGGTGTATCGTGACCCGGAATAGTTATATTTCTg                                 >  1:2200701/1‑60 (MQ=255)                  tGAAAACTCCAGCGCGGTCGGGTACGAGGTGTATCGTGACCCGGAATAGTTATATTTCTg                                 >  1:278356/1‑60 (MQ=255)                      aaCTCCAGCGCGGTCGGGTACGAGGTGTATCGTGACCCGGAATAGTTATATTTCTGGATCAg                           >  1:3716655/1‑62 (MQ=255)                      aaCTCCAGCGCGGTCGGGTACGAGGTGTATCGTGACCCGGAATAGTTATATTTCTGGATCAg                           >  1:2368275/1‑62 (MQ=255)                      aaCTCCAGCGCGGTCGGGTACGAGGTGTATCGTGACCCGGAATAGTTATATTTCTGGATCAg                           >  1:2486649/1‑62 (MQ=255)                      aaCTCCAGCGCGGTCGGGTACGAGGTGTATCGTGACCCGGAATAGTTATATTTCTGGATCAg                           >  1:41545/1‑62 (MQ=255)                      aaCTCCAGCGCGGTCGGGTACGAGGTGTATCGTGACCCGGAATAGTTATATTTCTGGATCAg                           >  1:2714374/1‑62 (MQ=255)                      aaCTCCAGCGCGGTCGGGTACGAGGTGTATCGTGACCCGGAATAGTTATATTTCTGGATCAg                           >  1:4320477/1‑62 (MQ=255)                               gcgGTCGGGTACGAGGTGTATCGTGACCCGGAATAGTTATATTTCTGGATCAGGGCCAGAt                   >  1:3591577/1‑61 (MQ=255)                               gcgGTCGGGTACGAGGTGTATCGTGACCCGGAATAGTTATATTTCTGGATCAGGGCCAGAt                   >  1:3726853/1‑61 (MQ=255)                               gcgGTCGGGTACGAGGTGTATCGTGACCCGGAATAGTTATATTTCTGGATCAGGGCCAGAt                   >  1:2499279/1‑61 (MQ=255)                               gcgGTCAGGTACGAGGTGTATCGTGACCCGGAATAGTTATATTTCTGGATCAGGGCCAGAt                   >  1:1247571/1‑61 (MQ=255)                                            aGGTGTATCGTGACCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAGTCGATTTg      <  1:3793787/61‑1 (MQ=255)                                             ggTGTATCGTGACCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAGTCGATTTGCt    <  1:4578367/62‑1 (MQ=255)                                             ggTGTATCGTGACCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAGTCGATTTGCt    <  1:1796427/62‑1 (MQ=255)                                               tgtATCGTGACCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAGTCGATTTGCTGt  <  1:4172475/62‑1 (MQ=255)                                               tgtATCGTGACCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAGTCGATTTGCTGt  <  1:4557929/62‑1 (MQ=255)                                               tgtATCGTGACCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAGTCGATTTGCTGt  <  1:4645050/62‑1 (MQ=255)                                               tgtATCGTGACCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAGTCGATTTGCTGt  <  1:6654/62‑1 (MQ=255)                                               tgtATCGTGACCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAGTCGATTTGCTGt  <  1:3592436/62‑1 (MQ=255)                                               tgtATCGTGACCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAGTCGATTTGCTGt  <  1:3062208/62‑1 (MQ=255)                                                     gTGACCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAg              <  1:3930520/44‑1 (MQ=255)                                                     gTGACCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAg              <  1:3481196/44‑1 (MQ=255)                                                     gTGACCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAg              <  1:3288289/44‑1 (MQ=255)                                                     gTGACCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAg              <  1:2620379/44‑1 (MQ=255)                                                     gTGACCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAg              <  1:2092801/44‑1 (MQ=255)                                                     gTGACCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAg              <  1:1484413/44‑1 (MQ=255)                                                     gTGACCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAg              <  1:1410374/44‑1 (MQ=255)                                                        |                                                     TGTTCGCCGAAGTCTTCTGAAAACTCCAGCGCGGTCGGGTACGAGGTGTATCGTGGCCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAGTCGATTTGCTGT  >  W3110S.gb/3584521‑3584628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑