Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,609,702	C→T	E151E (GAG→GAA)	fre ←	flavin reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,609,702	0	C	T	100.0%	106.1 / NA	34	E151E (GAG→GAA)	fre	flavin reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/27);  total (7/27)

CAACCACCGGCACCACTTGCAGACCAGGATGCTTCAACGAAAGCGCCTCAAGCTCGCAGAGATCATACAGATGCTGCTCTTCACGCCCGCCCCAGTAA  >  W3110S.gb/3609650‑3609747                                                     |                                              cAACCACCGGCACCACTTGCAGACCAGGATGCTTCAACGAAAGCGCCTCAAGTTCGCAgaga                                      <  1:3314265/62‑1 (MQ=255) cAACCACCGGCACCACTTGCAGACCAGGATGCTTCAACGAAAGCGCCTCAAGTTCGCAgaga                                      <  1:916929/62‑1 (MQ=255) cAACCACCGGCACCACTTGCAGACCAGGATGCTTCAACGAAAGCGCCTCAAGTTCGCAgaga                                      <  1:3929927/62‑1 (MQ=255) cAACCACCGGCACCACTTGCAGACCAGGATGCTTCAACGAAAGCGCCTCAAGTTCGCAgaga                                      <  1:4510704/62‑1 (MQ=255) cAACCACCGGCACCACTTGCAGACCAGGATGCTTCAACGAAAGCGCCTCAAGTTCGCAgaga                                      <  1:196572/62‑1 (MQ=255) cAACCACCGGCACCACTTGCAGACCAGGATGCTTCAACGAAAGCGCCTCAAGTTCGCAgaga                                      <  1:2042857/62‑1 (MQ=255) cAACCACCGGCACCACTTGCAGACCAGGATGCTTCAACGAAAGCGCCTCAAGTTCGCAgaga                                      <  1:2802738/62‑1 (MQ=255) cAACCACCGGCACCACTTGCAGACCAGGATGCTTCAACGAAAGCGCCTCAAGTTCGCAgaga                                      <  1:4055880/62‑1 (MQ=255) cAACCACCGGCACCACTTGCAGACCAGGATGCTTCAACGAAAGCGCCTCAAGTTCGCAgaga                                      <  1:3949961/62‑1 (MQ=255)    ccaccGGCACCACTTGCAGACCAGGATGCTTCAACGAAAGCGCCTCAAGTTCGCAGAGATCa                                   <  1:972222/62‑1 (MQ=255)    ccaccGGCACCACTTGCAGACCAGGATGCTTCAACGAAAGCGCCTCAAGTTCGCAGAGATCa                                   <  1:3101312/62‑1 (MQ=255)    ccaccGGCACCACTTGCAGACCAGGATGCTTCAACGAAAGCGCCTCAAGTTCGCAGAGATCa                                   <  1:4015420/62‑1 (MQ=255)         ggCACCACTTGCAGACCAGGATGCTTCAACGAAAGCGCCTCAAGTTCGCAGAGATCATACAg                              <  1:2469973/62‑1 (MQ=255)         ggCACCACTTGCAGACCAGGATGCTTCAACGAAAGCGCCTCAAGTTCGCAGAGATCATACAg                              <  1:355660/62‑1 (MQ=255)          gCACCACTTGCAGACCAGGATGCTTCAACGAAAGCGCCTCAAGTTCGCAGAGATCATACAg                              <  1:1803642/61‑1 (MQ=255)             ccacTTGCAGACCAGGATGCTTCAACGAAAGCGCCTCAAGTTCGCAGAGATCATACAGAtgc                          <  1:2059185/62‑1 (MQ=255)                                  tCAACGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCACGCCCGCCCCa      >  1:4587889/1‑61 (MQ=255)                                     aCGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCACGCCCGCCCCAGt    <  1:137134/60‑1 (MQ=255)                                     aCGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCACGCCCGCCCCAGt    <  1:3686584/60‑1 (MQ=255)                                     aCGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCACGCCCGCCCCAGTaa  >  1:2687581/1‑62 (MQ=255)                                     aCGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCACGCCCGCCCCAGTaa  >  1:2236011/1‑62 (MQ=255)                                     aCGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCACGCCCGCCCCAGTaa  >  1:4104179/1‑62 (MQ=255)                                     aCGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCACGCCCGCCCCAGTaa  >  1:4109271/1‑62 (MQ=255)                                     aCGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCACGCCCGCCCCAGTaa  >  1:1946559/1‑62 (MQ=255)                                     aCGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCACGCCCGCCCCAGTa   >  1:4512748/1‑61 (MQ=255)                                      cGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCACGCCCGCCCCAGt    <  1:4004194/59‑1 (MQ=255)                                      cGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCACGCCCGCCCCAGTaa  <  1:3507879/61‑1 (MQ=255)                                      cGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCACGCCCGCCCCAGTaa  <  1:3029195/61‑1 (MQ=255)                                      cGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCACGCCCGCCCCAGTaa  <  1:400056/61‑1 (MQ=255)                                      cGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCACGCCCGCCCCAGTaa  <  1:2581667/61‑1 (MQ=255)                                      cGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCACGCCCGCCCCAGTaa  <  1:1896364/61‑1 (MQ=255)                                      cGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCACGCCCGCCCCAGTaa  <  1:735786/61‑1 (MQ=255)                                      cGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCACGCCCGCCCCAGTaa  <  1:1673360/61‑1 (MQ=255)                                      cGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCACGCCCGCCCCAGTaa  <  1:1028722/61‑1 (MQ=255)                                                     |                                              CAACCACCGGCACCACTTGCAGACCAGGATGCTTCAACGAAAGCGCCTCAAGCTCGCAGAGATCATACAGATGCTGCTCTTCACGCCCGCCCCAGTAA  >  W3110S.gb/3609650‑3609747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑