Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,707,036	G→A	A483T (GCC→ACC)	yieN →	fused predicted transcriptional regulators

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,707,036	0	G	A	100.0%	72.6 / NA	23	A483T (GCC→ACC)	yieN	fused predicted transcriptional regulators
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/19);  total (4/19)

TCAGCGAACAGCAAAAATGTCTGTTTATCCCTGGCGACTGGTTAGGTCGCATTGAAGCCAGCCTACAGGATGTCGGTGCACAGATTCGCCAGGCACAACAATGCTAA  >  W3110S.gb/3706980‑3707086                                                         |                                                   tCAGCGAACAGCAAAAATGTCTGTTTATCCCTGGCGACTGGTTAGGTCGCATTGAaaccag                                                <  1:1005291/61‑1 (MQ=255) tCAGCGAACAGCAAAAATGTCTGTTTATCCCTGGCGACTGGTTAGGTCGCATTGAaaccag                                                <  1:456825/61‑1 (MQ=255) tCAGCGAACAGCAAAAATGTCTGTTTATCCCTGGCGACTGGTTAGGTCGCATTGAaaccag                                                <  1:829909/61‑1 (MQ=255) tCAGCGAACAGCAAAAATGTCTGTTTATCCCTGGCGACTGGTTAGGTCGCATTGAaaccag                                                <  1:744857/61‑1 (MQ=255) tCAGAGAACAGCAAAAATGTCTGTTTATCCCTGGCGACTGGTTAGGTCGCATTGAaaccag                                                <  1:1799158/61‑1 (MQ=255)  cAGCGAACAGCAAAAATGTCTGTTTATCCCTGGCGACTGGTTAGGTCGCATTGAaaccag                                                <  1:3521704/60‑1 (MQ=255)  cAGCGAACAGCAAAAATGTCTGTTTATCCCTGGCGACTGGTTAGGTCGCATTGAaaccag                                                <  1:3571080/60‑1 (MQ=255)  cAGCGAACAGCAAAAATGTCTGTTTATCCCTGGCGACTGGTTAGGTCGCATTGAaaccag                                                <  1:1785391/60‑1 (MQ=255)    gCGAACAGCAAAAATGTCTGTTTATCCCTGGCGACTGGTTAGGTCGCATTGAaaccag                                                <  1:4336404/58‑1 (MQ=255)          aGCAAAAATGTCTGTTTATCCCTGGCGACTGGTTAGGTCGCATTGAAACCAGCCTACAGGAt                                      <  1:2079013/62‑1 (MQ=255)          aGCAAAAATGTCTGTTTATCCCTGGCGACTGGTTAGGTCGCATTGAAACCAGCCTACAGGAt                                      <  1:2627653/62‑1 (MQ=255)          aGCAAAAATGTCTGTTTATCCCTGGCGACTGGTTAGGTCGCATTGAAACCAGCCTACAGGAt                                      <  1:3004079/62‑1 (MQ=255)                aaTGTCTGTTTATCCCTGGCGACTGGTTAGGTCGCATTGAAACCAGCCTACAGGATGTCgg                                 >  1:224132/1‑61 (MQ=255)                       gTTTATCCCTGGCGACTGGTTAGGTCGCATTGAAACCAGCCTACAg                                         >  1:3581089/1‑46 (MQ=255)                       gTTTATCCCTGGCGACTGGTTAGGTCGCATTGAAACCAGCCTACAg                                         >  1:1435205/1‑46 (MQ=255)                       gTTTATCCCTGGCGACTGGTTAGGTCGCATTGAAACCAGCCTACAg                                         >  1:4542580/1‑46 (MQ=255)                          tATCCCTGGCGACTGGTTAGGTCGCATTGAAACCAGCCTACAGGATGTCGGTGCACAGa                         <  1:427015/59‑1 (MQ=255)                          tATCCCTGGCGACTGGTTAGGTCGCATTGAAACCAGCCTACAGGATGTCGGTGCACAGa                         <  1:4080006/59‑1 (MQ=255)                          tATCCCTGGCGACTGGTTAGGTCGCATTGAAACCAGCCTACAGGATGTCGGTGCACAGa                         <  1:2890113/59‑1 (MQ=255)                          tATCCCTGGCGACTGGTTAGGTCGCATTGAAACCAGCCTACAGGATGTCGGTGCACAGa                         <  1:246362/59‑1 (MQ=255)                          tATCCCTGGCGACTGGTTAGGTCGCATTGAAACCAGCCTACAGGATGTCGGTGCACAGa                         <  1:1625863/59‑1 (MQ=255)                          tATCCCTGGCGACTGGTTAGGTCGCATTGAAACCAGCCTACAGGATGTCGGTGCACAGa                         <  1:1292342/59‑1 (MQ=255)                             cccTGGCGACTGGTTAGGTCGCATTGAAACCAGCCTACAGGATGTCGGTGCACAGATTCGc                    <  1:91015/61‑1 (MQ=255)                             cccTGGCGACTGGTTAGGTCGCATTGAAACCAGCCTACAGGATGTCGGTGCACAGATTCGc                    <  1:324621/61‑1 (MQ=255)                                     aCTGGTTAGGTCGCATTGAAACCAGCCTACAGGATGTCGGTGCACAGATTCGCCAGGCacaa           <  1:388753/62‑1 (MQ=255)                                     aCTGGTTAGGTCGCATTGAAACCAGCCTACAGGATGTCGGTGCACAGATTCGCCAGGCacaa           <  1:4402245/62‑1 (MQ=255)                                     aCTGGTTAGGTCGCATTGAAACCAGCCTACAGGATGTCGGTGCACAGATTCGCCAGGCacaa           <  1:2331196/62‑1 (MQ=255)                                     aCTGGTTAGGTCGCATTGAAACCAGCCTACAGGATGTCGGTGCACAGATTCGCCAGGCacaa           <  1:1119065/62‑1 (MQ=255)                                               tCGCATTGAAACCAGCCTACAGGATGTCGGTGCACAGATTCGCCAGGCACAACAATGCTaa  <  1:1689260/61‑1 (MQ=255)                                                      gAAACCAGCCTACAGGATGTCGGTGCACAGATTCGCCAGGCacaa           <  1:2760333/45‑1 (MQ=255)                                                      gAAACCAGCCTACAGGATGTCGGTGCACAGATTCGCCAGGCacaa           <  1:2666181/45‑1 (MQ=255)                                                      gAAACCAGCCTACAGGATGTCGGTGCACAGATTCGCCAGGCacaa           <  1:824858/45‑1 (MQ=255)                                                         |                                                   TCAGCGAACAGCAAAAATGTCTGTTTATCCCTGGCGACTGGTTAGGTCGCATTGAAGCCAGCCTACAGGATGTCGGTGCACAGATTCGCCAGGCACAACAATGCTAA  >  W3110S.gb/3706980‑3707086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑