Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,727,747	T→C	V168V (GTT→GTC)	pstA →	phosphate transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,727,747	0	T	C	100.0%	125.2 / NA	34	V168V (GTT→GTC)	pstA	phosphate transporter subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/19);  total (15/19)

CGGCTGGGCGGGCGTGATTGCCCTGGCGTTGTTGCAGGTGCCGATTGTTATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCTGCGTGA  >  W3110S.gb/3727699‑3727797                                                 |                                                   cGGCTGGGCGGGCGTGATTGCCCTGGCGTTGTTGCAGGTGCCGATTGTCATCCGcacca                                          >  1:1879028/1‑59 (MQ=255)   gCTGGGCGGGCGTGATTGCCCTGGCGTTGTTGCAGGTGCCGATTGTCATCCGCACCACCg                                       <  1:1476252/60‑1 (MQ=255)   gCTGGGCGGGCGTGATTGCCCTGGCGTTGTTGCAGGTGCCGATTGTCATCCGCACCACCg                                       <  1:2429049/60‑1 (MQ=255)   gCTGGGCGGGCGTGATTGCCCTGGCGTTGTTGCAGGTGCCGATTGTCATCCGCACCACCg                                       <  1:4574027/60‑1 (MQ=255)   gCTGGGCGGGCGTGATTGCCCTGGCGTTGTTGCAGGTGCCGATTGTCATCCGCACCACCg                                       <  1:4584624/60‑1 (MQ=255)    cTGGGCGGGCGTGATTGCCCTGGCGTTGTTGCCGGTGCCGATTGTCATCCGCACCACCgag                                     >  1:3386283/1‑61 (MQ=255)    cTGGGCGGGCGTGATTGCCCTGGCGTTGTTGCAGGTGCCGATTGTCATCCGCACCACCgag                                     >  1:4548990/1‑61 (MQ=255)    cTGGGCGGGCGTGATTGCCCTGGCGTTGTTGCAGGTGCCGATTGTCATCCGCACCACCgag                                     >  1:2598807/1‑61 (MQ=255)    cTGGGCGGGCGTGATTGCCCTGGCGTTGTTGCACGTGCCGATTGTCATCCGCACCACCgag                                     >  1:2058433/1‑61 (MQ=255)     tGGGCGGGCGTGATTGCCCTGGCGTTGTTGCAGGTGCCGATTGTCATCCGCACCACCGAGaa                                   >  1:909759/1‑62 (MQ=255)     tGGGCGGGCGTGATTGCCCTGGCGTTGTTGCAGGTGCCGATTGTCATCCGCACCACCGAGaa                                   >  1:4302591/1‑62 (MQ=255)     tGGGCGGGCGTGATTGCCCTGGCGTTGTTGCAGGTGCCGATTGTCATCCGCACCACCGAGaa                                   >  1:899385/1‑62 (MQ=255)     tGGGCGGGCGTGATTGCCCTGGCGTTGTTGCAGGTGCCGAATGTCATCCGCACCACCGAGaa                                   >  1:4174051/1‑62 (MQ=255)      gggcgggcGTGATTGCCCTGGCGTTGTTGCAGGTGCCGATTGTCATCCGCACCACCGAGAAc                                  <  1:1107643/62‑1 (MQ=255)          gggcgTGATTGCCCTGGCGTTGTTGCAGGTGCCGATTGTCATCCGCACCACCGAGAACATGc                              <  1:2155237/62‑1 (MQ=255)          gggcgTGATTGCCCTGGCGTTGTTGCAGGTGCCGATTGTCATCCGCACCACCGAGAACATGc                              <  1:1439251/62‑1 (MQ=255)          gggcgTGATTGCCCTGGCGTTGTTGCAGGTGCCGATTGTCATCCGCACCACCGAGAACATGc                              <  1:3078425/62‑1 (MQ=255)                    gCCCTGGCGTTGTTGCAGGTGCCGATTGTCATCCGCACCACCGAGAACATCCTGAAACTgg                     >  1:2889105/1‑61 (MQ=255)                           cGTTGTTGCAGGTGCCGATTGTCATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTAc             >  1:4144051/1‑62 (MQ=255)                                ttgCAGGTGCCGATTGTCATCCGCACCACCGAGAACATTCTGAAACTGGTGCCGTACAGCCt        >  1:1991054/1‑62 (MQ=255)                                ttgCAGGTGCCGATTGTCATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCt        >  1:2826783/1‑62 (MQ=255)                                      gggCCGATTGTCATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCTGCGTGa  <  1:2556522/60‑1 (MQ=255)                                      gTGCCGATTGTCATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCTGCGTGa  <  1:4527318/62‑1 (MQ=255)                                      gTGCCGATTGTCATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCTGCGTGa  <  1:2953586/62‑1 (MQ=255)                                      gTGCCGATTGTCATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCTGCGTGa  <  1:881326/62‑1 (MQ=255)                                      gTGCCGATTGTCATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCTGCGTGa  <  1:4521971/62‑1 (MQ=255)                                      gTGCCGATTGTCATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCTGCGTGa  <  1:3962055/62‑1 (MQ=255)                                      gTGCCGATTGTCATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCTGCGTGa  >  1:2423197/1‑62 (MQ=255)                                      gTGCCGATTGTCATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCTGCGTGa  <  1:2176901/62‑1 (MQ=255)                                      gTGCCGATTGTCATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCTGCGTGa  <  1:2171320/62‑1 (MQ=255)                                      gTGCCGATTGTCATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCTGCGTGa  >  1:1394015/1‑62 (MQ=255)                                      gTGCCGATTGTCATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCTGCGTGa  <  1:1232553/62‑1 (MQ=255)                                       tGCCGATTGTCATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCTGCGTGa  <  1:431855/61‑1 (MQ=255)                                              tGTCATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCTGCGTGa  <  1:358001/54‑1 (MQ=255)                                                 |                                                   CGGCTGGGCGGGCGTGATTGCCCTGGCGTTGTTGCAGGTGCCGATTGTTATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCTGCGTGA  >  W3110S.gb/3727699‑3727797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑