Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,735,429 G→A A323T (GCA→ACA)  bglH → carbohydrate‑specific outer membrane porin, cryptic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,735,4290GA100.0% 389.7 / NA 118A323T (GCA→ACA) bglHcarbohydrate‑specific outer membrane porin, cryptic
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (65/53);  total (65/53)

Only 100 of 121 total aligned reads displayed.

ATGGGATAAAGCCACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGATAC  >  W3110S.gb/3735374‑3735481
                                                       |                                                    
aTGGGATAAAGCCACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGcaca                                                    <  1:650318/58‑1 (MQ=255)
aTGGGATAAAGCCACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGcaca                                                    <  1:2001837/58‑1 (MQ=255)
aTGGGATAAAGCCACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCACATc                                                  >  1:2492428/1‑60 (MQ=255)
aTGGGATAAAGCCACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCACATCg                                                 >  1:2498146/1‑61 (MQ=255)
  gggATAAAGCCACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCACATCGGa                                               <  1:1110756/61‑1 (MQ=255)
  gggATAAAGCCACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCACATCGGa                                               <  1:1253415/61‑1 (MQ=255)
  gggATAAAGCCACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCACATCGGa                                               <  1:2178848/61‑1 (MQ=255)
       aaaGCCACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCACATCGGaaaaa                                           >  1:1773296/1‑60 (MQ=255)
       aaaGCCACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAg                                          >  1:3260943/1‑61 (MQ=255)
       aaaGCCACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAg                                          >  1:3186741/1‑61 (MQ=255)
       aaaGCCACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAg                                          >  1:4225962/1‑61 (MQ=255)
       aaaGCCACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAg                                          >  1:237986/1‑61 (MQ=255)
       aaaGCCACCTTAATGGTAAGTGGTCGATAAGTCACGGCAAACGAAAGCACATCGGAAAAAg                                          >  1:2247956/1‑61 (MQ=255)
            cACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATc                                    >  1:2249013/1‑62 (MQ=255)
            cACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATc                                    >  1:2894380/1‑62 (MQ=255)
            cACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATc                                    >  1:3119728/1‑62 (MQ=255)
            cACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATc                                    >  1:2585017/1‑62 (MQ=255)
            cACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATc                                    >  1:2542274/1‑62 (MQ=255)
            cACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATc                                    >  1:23797/1‑62 (MQ=255)
             aCCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATCa                                   <  1:3217747/62‑1 (MQ=255)
             aCCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATCa                                   <  1:3470171/62‑1 (MQ=255)
             aCCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATCa                                   <  1:622821/62‑1 (MQ=255)
              ccTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATCAg                                  <  1:1983558/62‑1 (MQ=255)
               cTTAATGGTAAGTGGTCGTTATGTCACGGCATACGAAAGCACATCGGAAAAAGATAATCAg                                  >  1:1280875/1‑61 (MQ=255)
               cTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATCAg                                  >  1:4684874/1‑61 (MQ=255)
               cTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATCAg                                  >  1:4602354/1‑61 (MQ=255)
               cTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATCAg                                  <  1:2727968/61‑1 (MQ=255)
               cTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATCAg                                  >  1:15048/1‑61 (MQ=255)
                      gTAAGTGGTCGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGAtaataa                          <  1:1961697/62‑1 (MQ=255)
                      gTAAGTGGTCGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGAtaataa                          <  1:2332164/62‑1 (MQ=255)
                      gTAAGTGGTCGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGAtaataa                          <  1:1465970/62‑1 (MQ=255)
                      gTAAGTGGTCGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGAtaataa                          <  1:3927982/62‑1 (MQ=255)
                      gTAAGTGGTCGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGAtaataa                          <  1:3287355/62‑1 (MQ=255)
                       tAAGTGGTCGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGAtaataa                          <  1:3663413/61‑1 (MQ=255)
                       tAAGTGGTCGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAAc                         >  1:2814472/1‑62 (MQ=255)
                               cGTTATGTCACGGCAAACGAAATCACATCGGAAAAAGATAATCAGGATAATAACggg                      <  1:3837418/57‑1 (MQ=255)
                               cGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATCa                                   <  1:4548612/44‑1 (MQ=255)
                               cGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATCa                                   <  1:93682/44‑1 (MQ=255)
                               cGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATCa                                   <  1:1885120/44‑1 (MQ=255)
                               cGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATCa                                   <  1:1813044/44‑1 (MQ=255)
                               cGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACggg                      <  1:3535188/57‑1 (MQ=255)
                               cGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACggg                      <  1:2036972/57‑1 (MQ=255)
                               cGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACggg                      <  1:1838546/57‑1 (MQ=255)
                               cGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACggg                      <  1:2956414/57‑1 (MQ=255)
                               cGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACggg                      <  1:3420437/57‑1 (MQ=255)
                               cGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACggg                      <  1:351385/57‑1 (MQ=255)
                               cGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACggg                      <  1:1754079/57‑1 (MQ=255)
                               cGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACggg                      <  1:1551331/57‑1 (MQ=255)
                               cGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACggg                      <  1:4686531/57‑1 (MQ=255)
                               cGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACggg                      <  1:1381734/57‑1 (MQ=255)
                               cGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGtatt                  >  1:2326443/1‑61 (MQ=255)
                               cGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGtatt                  >  1:1633911/1‑61 (MQ=255)
                               cGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGtatt                  >  1:4379867/1‑61 (MQ=255)
                               cGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGtatt                  >  1:3337027/1‑61 (MQ=255)
                               cGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGtatt                  >  1:1442600/1‑61 (MQ=255)
                               cGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGtatt                  >  1:2068987/1‑61 (MQ=255)
                               cGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGtatt                  >  1:2249028/1‑61 (MQ=255)
                               cGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGtat                   >  1:4501632/1‑60 (MQ=255)
                                      tCACGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTg           <  1:2546535/61‑1 (MQ=255)
                                      tCACGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTg           <  1:4428567/61‑1 (MQ=255)
                                      tCACGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTg           <  1:4486052/61‑1 (MQ=255)
                                      tCACGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTg           <  1:4612139/61‑1 (MQ=255)
                                      tCACGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTg           <  1:1139366/61‑1 (MQ=255)
                                      tCACGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTg           <  1:825605/61‑1 (MQ=255)
                                       cACGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTg           >  1:1813218/1‑60 (MQ=255)
                                         cGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGTATTATg               >  1:2250664/1‑54 (MQ=255)
                                         cGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGTATTATg               >  1:111373/1‑54 (MQ=255)
                                         cGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGTATTATg               >  1:2358880/1‑54 (MQ=255)
                                         cGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGTATTATg               >  1:4257580/1‑54 (MQ=255)
                                         cGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGTATTATg               >  1:1572177/1‑54 (MQ=255)
                                         cGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGTATTATg               >  1:4120356/1‑54 (MQ=255)
                                         cGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGTATTATg               >  1:1743206/1‑54 (MQ=255)
                                         cGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGTATTATg               >  1:364082/1‑54 (MQ=255)
                                         cGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGTATTATg               >  1:3057791/1‑54 (MQ=255)
                                         cGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGTATTATg               >  1:3278882/1‑54 (MQ=255)
                                         cGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGTATTATg               >  1:3587080/1‑54 (MQ=255)
                                         cGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGTATTATg               >  1:3450131/1‑54 (MQ=255)
                                          ggCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGTAAAg      <  1:2290463/62‑1 (MQ=255)
                                          ggCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGaaa       >  1:854986/1‑61 (MQ=255)
                                          ggCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGaaa       >  1:4027469/1‑61 (MQ=255)
                                          ggCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAg      <  1:2792761/62‑1 (MQ=255)
                                             aaaCGAAAGCACATCGGAAAAAGATAATCAGGataa                             >  1:2682423/1‑36 (MQ=255)
                                             aaaCGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGa     >  1:3160497/1‑60 (MQ=255)
                                             aaaCGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGATa   >  1:3715848/1‑62 (MQ=255)
                                             aaaCGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGATa   >  1:1410723/1‑62 (MQ=255)
                                             aaaCGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGATa   >  1:4410918/1‑62 (MQ=255)
                                             aaaCGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGATa   >  1:40923/1‑62 (MQ=255)
                                             aaaCGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGATa   >  1:3980683/1‑62 (MQ=255)
                                              aaCGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGa              >  1:207713/1‑50 (MQ=255)
                                              aaCGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGATAc  <  1:34384/62‑1 (MQ=255)
                                              aaCGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGATAc  >  1:2119999/1‑62 (MQ=255)
                                              aaCGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGATAc  <  1:3370564/62‑1 (MQ=255)
                                              aaCGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGATAc  <  1:3351888/62‑1 (MQ=255)
                                              aaCGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGATAc  >  1:2182873/1‑62 (MQ=255)
                                              aaCGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGATAc  <  1:411076/62‑1 (MQ=255)
                                              aaCGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGATAc  >  1:622291/1‑62 (MQ=255)
                                              aaCGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGATAc  >  1:2968000/1‑62 (MQ=255)
                                              aaCGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGATAc  <  1:83189/62‑1 (MQ=255)
                                              aaCGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGATAc  >  1:2659824/1‑62 (MQ=255)
                                              aaCGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGATAc  >  1:2665376/1‑62 (MQ=255)
                                                       |                                                    
ATGGGATAAAGCCACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGATAC  >  W3110S.gb/3735374‑3735481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑