BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,739,327	C→T	V195I (GTT→ATT)	yieH ←	predicted hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,739,327	0	C	T	100.0%	449.0 / NA	134	V195I (GTT→ATT)	yieH	predicted hydrolase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (60/74); total (60/74)

Only 100 of 134 total aligned reads displayed.

CTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCG  >  W3110S.gb/3739270‑3739379

cTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATc                                                  <  1:214050/62‑1 (MQ=255)

cTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATc                                                  <  1:3582479/62‑1 (MQ=255)

cTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATc                                                  <  1:3515620/62‑1 (MQ=255)

cTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATc                                                  <  1:1106261/62‑1 (MQ=255)

cTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATc                                                  <  1:808760/62‑1 (MQ=255)

cTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATc                                                  <  1:4153485/62‑1 (MQ=255)

cTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATc                                                  <  1:1835064/62‑1 (MQ=255)

cTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATc                                                  <  1:1808/62‑1 (MQ=255)

cTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATc                                                  <  1:667472/62‑1 (MQ=255)

cTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATc                                                  <  1:2395002/62‑1 (MQ=255)

cTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATc                                                  <  1:1575251/62‑1 (MQ=255)

cTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATc                                                  <  1:1495044/62‑1 (MQ=255)

cTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATc                                                  <  1:248799/62‑1 (MQ=255)

 tttCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATc                                                  <  1:3067575/61‑1 (MQ=255)

 tttCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATc                                                  <  1:79627/61‑1 (MQ=255)

 tttCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCg                                                 >  1:3438129/1‑62 (MQ=255)

 tttCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGGGGTGACTTTCGGGTGAATGATc                                                  <  1:680987/61‑1 (MQ=255)

  ttCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCgg                                                <  1:3069057/62‑1 (MQ=255)

  ttCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCgg                                                <  1:1307616/62‑1 (MQ=255)

                   cTGCGAAAGATGGGTAAAGGTGGTGACTTGCGGGTGAATGATCGGCTTATTGTGCGGGTCgg                               >  1:210127/1‑62 (MQ=255)

                        aaaGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTAt                                           >  1:485031/1‑45 (MQ=255)

                        aaaGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGgcgc                            >  1:4440142/1‑60 (MQ=255)

                        aaaGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGgcgc                            >  1:848003/1‑60 (MQ=255)

                        aaaGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGgcgc                            >  1:3958167/1‑60 (MQ=255)

                        aaaGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGgcgc                            >  1:897481/1‑60 (MQ=255)

                         aaGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGa                         >  1:2215558/1‑62 (MQ=255)

                           gATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGa                         >  1:3566267/1‑60 (MQ=255)

                           gATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGa                         >  1:1032803/1‑60 (MQ=255)

                           gATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGa                         >  1:1978080/1‑60 (MQ=255)

                           gATGGGTAAAGGTGATGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGa                         >  1:3232205/1‑60 (MQ=255)

                               ggTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGa                   <  1:4400057/62‑1 (MQ=255)

                               ggTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGa                   <  1:2434117/62‑1 (MQ=255)

                               ggTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGa                   <  1:3024172/62‑1 (MQ=255)

                               ggTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGa                   <  1:2976506/62‑1 (MQ=255)

                                gTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGt                      <  1:1705645/58‑1 (MQ=255)

                                gTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGt                      <  1:2498525/58‑1 (MQ=255)

                                gTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGt                      <  1:1483242/58‑1 (MQ=255)

                                gTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGt                      <  1:2722087/58‑1 (MQ=255)

                                  aaaGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAaca                <  1:3648402/62‑1 (MQ=255)

                                  aaaGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAaca                <  1:4109575/62‑1 (MQ=255)

                                  aaaGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAaca                <  1:426315/62‑1 (MQ=255)

                                  aaaGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAaca                <  1:3104013/62‑1 (MQ=255)

                                  aaaGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAaca                <  1:4445698/62‑1 (MQ=255)

                                  aaaGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAaca                <  1:965493/62‑1 (MQ=255)

                                  aaaGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAaca                <  1:2119469/62‑1 (MQ=255)

                                  aaaGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAaca                <  1:2577889/62‑1 (MQ=255)

                                  aaaGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAaca                <  1:1774110/62‑1 (MQ=255)

                                  aaaGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAaca                <  1:1482469/62‑1 (MQ=255)

                                  aaaGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAaca                <  1:1985580/62‑1 (MQ=255)

                                    aGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACt              >  1:3247866/1‑62 (MQ=255)

                                    aGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACt              >  1:3479898/1‑62 (MQ=255)

                                     ggtggtGACTTTCGGGTGAATGATCGGCTTATtgtg                                       >  1:3712250/1‑36 (MQ=255)

                                     ggtggtGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACt              >  1:426408/1‑61 (MQ=255)

                                     ggtggtGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACt              >  1:774167/1‑61 (MQ=255)

                                     ggtggtGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACt              >  1:3173004/1‑61 (MQ=255)

                                     ggtggtGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACt              >  1:3511857/1‑61 (MQ=255)

                                       tggtgACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAacac               >  1:3374194/1‑58 (MQ=255)

                                       tggtgACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAacac               >  1:689723/1‑58 (MQ=255)

                                       tggtgACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAacac               >  1:375932/1‑58 (MQ=255)

                                       tggtgACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAacac               >  1:622382/1‑58 (MQ=255)

                                       tggtgACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAacac               >  1:2260877/1‑58 (MQ=255)

                                          tgACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTc            >  1:1154107/1‑58 (MQ=255)

                                          tgACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTc            >  1:2734052/1‑58 (MQ=255)

                                          tgACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTc            >  1:2088849/1‑58 (MQ=255)

                                          tgACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTc            >  1:4162358/1‑58 (MQ=255)

                                          tgACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTc            >  1:1680093/1‑58 (MQ=255)

                                          tgACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTc            >  1:4516161/1‑58 (MQ=255)

                                          tgACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTc            >  1:1500924/1‑58 (MQ=255)

                                          tgACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTc            >  1:218394/1‑58 (MQ=255)

                                          tgACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTc            >  1:2212638/1‑58 (MQ=255)

                                          tgACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTc            >  1:112656/1‑58 (MQ=255)

                                          tgACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTc            >  1:2408080/1‑58 (MQ=255)

                                          tgACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTc            >  1:3099338/1‑58 (MQ=255)

                                          tgACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTc            >  1:3093250/1‑58 (MQ=255)

                                          tgACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTc            >  1:104295/1‑58 (MQ=255)

                                           gACTTTCGGGTGAATGATCGGCTTTTTGTGCGGGTCGGCGCAGAAGTAGAacac               <  1:2177909/54‑1 (MQ=255)

                                           gACTTTCGGGTGAATGATCGGCTTATTGTGTGGGTCGGCGCAGAAGTAGAacac               <  1:838174/54‑1 (MQ=255)

                                           gACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAacac               <  1:1254524/54‑1 (MQ=255)

                                           gACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAacac               <  1:1464711/54‑1 (MQ=255)

                                           gACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAacac               <  1:4003167/54‑1 (MQ=255)

                                           gACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAacac               <  1:524869/54‑1 (MQ=255)

                                           gACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAacac               <  1:963341/54‑1 (MQ=255)

                                           gACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAacac               <  1:1010249/54‑1 (MQ=255)

                                           gACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAacac               <  1:2614717/54‑1 (MQ=255)

                                           gACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAacac               <  1:4209116/54‑1 (MQ=255)

                                           gACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAacac               <  1:1942403/54‑1 (MQ=255)

                                           gACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAacac               <  1:2564764/54‑1 (MQ=255)

                                           gACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAacac               <  1:3593582/54‑1 (MQ=255)

                                           gACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATAc       <  1:1502552/62‑1 (MQ=255)

                                           gACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATAc       <  1:2840029/62‑1 (MQ=255)

                                           gACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATAc       <  1:3422255/62‑1 (MQ=255)

                                           gACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATAc       <  1:4502961/62‑1 (MQ=255)

                                              tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAacac               <  1:2687016/51‑1 (MQ=255)

                                              tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt     <  1:4517833/61‑1 (MQ=255)

                                                tCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGc   >  1:1079501/1‑61 (MQ=255)

                                                tCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCg  >  1:2141692/1‑62 (MQ=255)

                                                tCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCg  >  1:371782/1‑62 (MQ=255)

                                                tCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCg  >  1:4033634/1‑62 (MQ=255)

                                                  gggTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCg  <  1:3626164/60‑1 (MQ=255)

                                                  gggTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCg  <  1:3631534/60‑1 (MQ=255)

CTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCG  >  W3110S.gb/3739270‑3739379

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`