Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,806,473	G→A	P198P (CCG→CCA)	yicI →	predicted alpha‑glucosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,806,473	0	G	A	100.0%	318.1 / NA	94	P198P (CCG→CCA)	yicI	predicted alpha‑glucosidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (39/55);  total (39/55)

AGACCTGGAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGTCT  >  W3110S.gb/3806418‑3806528                                                        |                                                        agaCCTGGAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTAc                                                   >  1:2965896/1‑62 (MQ=255) agaCCTGGAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTAc                                                   >  1:2741551/1‑62 (MQ=255) agaCCTGGAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTAc                                                   >  1:1573649/1‑62 (MQ=255) agaCCTGGAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTAc                                                   >  1:2050350/1‑62 (MQ=255) agaCCTGGAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTAc                                                   >  1:4618057/1‑62 (MQ=255)  gaCCTGGAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTAc                                                   <  1:2944495/61‑1 (MQ=255)  gaCCTGGAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTAc                                                   <  1:2952325/61‑1 (MQ=255)  gaCCTGGAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTAc                                                   <  1:1392007/61‑1 (MQ=255)  gaCCTGGAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTAc                                                   <  1:3359686/61‑1 (MQ=255)  gaCCTGGAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTAc                                                   <  1:2255227/61‑1 (MQ=255)  gaCCTGGAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTAc                                                   <  1:4114852/61‑1 (MQ=255)  gaCCTGGAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTAc                                                   <  1:439591/61‑1 (MQ=255)  gaCCTGGAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTAc                                                   <  1:1524592/61‑1 (MQ=255)  gaCCTGGAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTAc                                                   <  1:527935/61‑1 (MQ=255)  gaCCTGGAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTAc                                                   <  1:1789970/61‑1 (MQ=255)       ggAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTa                                                    <  1:1120886/55‑1 (MQ=255)         aaCCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTACATGACt                                             >  1:227848/1‑60 (MQ=255)         aaCCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTa                                            >  1:218261/1‑61 (MQ=255)         aaCCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTa                                            >  1:3107562/1‑61 (MQ=255)         aaCCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTa                                            >  1:1718095/1‑61 (MQ=255)          aCCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTaa                                           <  1:1754970/61‑1 (MQ=255)          aCCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTaa                                           <  1:1650525/61‑1 (MQ=255)          aCCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTaa                                           <  1:832708/61‑1 (MQ=255)             gggACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTACATTACTAACCGt                                       <  1:4604417/62‑1 (MQ=255)             gggACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTAACCGt                                       <  1:115728/62‑1 (MQ=255)             gggACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTAACCGt                                       <  1:1413334/62‑1 (MQ=255)             gggACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTAACCGt                                       <  1:4025716/62‑1 (MQ=255)             gggACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTAACCGt                                       <  1:2763068/62‑1 (MQ=255)             gggACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTAACCGt                                       <  1:1438199/62‑1 (MQ=255)             gggACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTAACCGt                                       <  1:3534532/62‑1 (MQ=255)              ggACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTACAt                                                 >  1:3577233/1‑51 (MQ=255)              ggACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTACAt                                                 >  1:2663582/1‑51 (MQ=255)              ggACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTACAt                                                 >  1:2623962/1‑51 (MQ=255)              ggACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTACAt                                                 >  1:1491420/1‑51 (MQ=255)              ggACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTACAt                                                 >  1:1501484/1‑51 (MQ=255)              ggACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTACATGACt                                             >  1:1487773/1‑55 (MQ=255)              ggACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTACATGACt                                             >  1:3914666/1‑55 (MQ=255)              ggACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTACATGACt                                             >  1:2870968/1‑55 (MQ=255)              ggACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTACATGACt                                             >  1:2934915/1‑55 (MQ=255)              ggACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTAACCGTg                                      <  1:3146717/62‑1 (MQ=255)              ggACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTAACCGTg                                      <  1:3659234/62‑1 (MQ=255)              ggACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTAACCGTg                                      <  1:495266/62‑1 (MQ=255)              ggACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTAACCGTg                                      <  1:3020837/62‑1 (MQ=255)              ggACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTAACCGTg                                      <  1:1705827/62‑1 (MQ=255)                aCGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTAACCGTGGt                                    <  1:2183491/62‑1 (MQ=255)                aCGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTAACCGTGGt                                    <  1:3889881/62‑1 (MQ=255)                aCGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTAACCGTGGt                                    <  1:3364702/62‑1 (MQ=255)                aCGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTAACCGTGGt                                    <  1:1726881/62‑1 (MQ=255)                aCGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTAACCGTGGt                                    <  1:2730976/62‑1 (MQ=255)                    cggcACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTAACCGTGGTTATg                                >  1:4068635/1‑62 (MQ=255)                    cggcACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTAACCGTGGTTATg                                >  1:3481614/1‑62 (MQ=255)                    cggcACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTAACCGTGGTTATg                                >  1:2023327/1‑62 (MQ=255)                    cggcACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTAACCGTGGTTATg                                >  1:3163485/1‑62 (MQ=255)                    cggcACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTAACCGTGGTTATg                                >  1:3132635/1‑62 (MQ=255)                    cggcACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTAACCGTGGTTATg                                >  1:1703390/1‑62 (MQ=255)                    cggcACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTAACCGTGGTTATg                                >  1:64705/1‑62 (MQ=255)                    cggcACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTAACCGTGGTTATg                                >  1:651473/1‑62 (MQ=255)                      gcACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTAACCGTGGTTATggg                              <  1:8585/62‑1 (MQ=255)                       cacaAGTACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTAACCGTGGTTATgggg                             >  1:2244303/1‑62 (MQ=255)                           aGTACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTaa                                           <  1:2700300/44‑1 (MQ=255)                           aGTACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTaa                                           <  1:6826/44‑1 (MQ=255)                           aGTACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTaa                                           <  1:4167488/44‑1 (MQ=255)                           aGTACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTaa                                           <  1:88449/44‑1 (MQ=255)                           aGTACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTaa                                           <  1:2461027/44‑1 (MQ=255)                             tACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTAAc                                          <  1:4012180/43‑1 (MQ=255)                             tACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTAAc                                          <  1:4424926/43‑1 (MQ=255)                             tACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTAAc                                          <  1:4476100/43‑1 (MQ=255)                             tACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTAAc                                          <  1:4513114/43‑1 (MQ=255)                             tACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTAAc                                          <  1:648015/43‑1 (MQ=255)                             tACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTAAc                                          <  1:592970/43‑1 (MQ=255)                             tACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTAAc                                          <  1:2888386/43‑1 (MQ=255)                             tACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTAAc                                          <  1:3007263/43‑1 (MQ=255)                             tACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTAAc                                          <  1:3161406/43‑1 (MQ=255)                             tACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTAAc                                          <  1:3223646/43‑1 (MQ=255)                             tACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTAAc                                          <  1:2562372/43‑1 (MQ=255)                             tACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTAAc                                          <  1:3385925/43‑1 (MQ=255)                              aCTGAACAGGCGTATAAAAATATCCCATTCTACATGACTAAc                                          <  1:4405339/42‑1 (MQ=255)                               cTGAACAGGCGTATAAAAATATCGCATTCTTCATGACTAAc                                          <  1:3519169/41‑1 (MQ=37)                                  aaCAGGCGTATAAAAATATCCCATTCTACATGACTAACCGTGGt                                    >  1:2944435/1‑44 (MQ=255)                                  aaCAGGCGTATAAAAATATCCCATTCTACATGACTAACCGTGGt                                    >  1:2620242/1‑44 (MQ=255)                                  aaCAGGCGTATAAAAATATCCCATTCTACATGACTAACCGTGGt                                    >  1:4456645/1‑44 (MQ=255)                                  aaCAGGCGTATAAAAATATCCCATTCTACATGACTAACCGTGGt                                    >  1:2283955/1‑44 (MQ=255)                                  aaCAGGCGTATAAAAATATCCCATTCTACATGACTAACCGTGGt                                    >  1:4140679/1‑44 (MQ=255)                                  aaCAGGCGTATAAAAATATCCCATTCTACATGACTAACCGTGGt                                    >  1:2263690/1‑44 (MQ=255)                                  aaCAGGCGTATAAAAATATCCCATTCTACATGACTAACCGTGGTTATGGGGTACTGGTCaa                   >  1:3314897/1‑61 (MQ=255)                                  aaCAGGCGTATAAAAATATCCCATTCTACATGACTAACCGTGGTTATGGGGTACTGGTCaa                   >  1:308625/1‑61 (MQ=255)                                  aaCAGGCGTATAAAAATATCCCATTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAat                  >  1:3917441/1‑62 (MQ=255)                                  aaCAGGCGTATAAAAATATCCCATTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAat                  >  1:3117431/1‑62 (MQ=255)                                  aaCAGGCGTATAAAAATATCCCATTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAat                  >  1:1028069/1‑62 (MQ=255)                                  aaCAGGCGTATAAAAATATCCCATTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAat                  >  1:2911417/1‑62 (MQ=255)                                                 atatCCCATTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGtc   <  1:622017/62‑1 (MQ=255)                                                 atatCCCATTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGtc   <  1:2795791/62‑1 (MQ=255)                                                 atatCCCATTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGtc   <  1:829983/62‑1 (MQ=255)                                                  tatCCCATTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGtct  <  1:1507138/62‑1 (MQ=255)                                                        |                                                        AGACCTGGAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGTCT  >  W3110S.gb/3806418‑3806528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑